Usefulness of the Hemagglutination Test Using Treponema Pallidum Antigen (Tpha) for the Serodiagnosis of Syphilis

Treponema pallidum haemagglutination test for syphilis. Evaluation of a modified micro-method.

“…Its application to the serological diagnosis of syphilis was first reported by Rathlev (1965Rathlev ( , 1967, using a haemagglutination technique for the detection of specific antitreponemal antibody in which formolized tanned sheep cells sensitized with ultrasonicated material from pathogenic Treponema pallidum (Nichols strain) were used as antigen. This was modified by Tomizawa and Kasamatsu (1966) and Tomizawa, Kasamatsu, and Yamaya (1969), who introduced a different absorbing reagent to remove cross-reacting antibodies. The T. pallidum Haemagglutination Assay (TPHIA) was originally described as a macro-method, reagents for which are available commercially.…”

Treponema Pallidum Immune Adherence Haemagglutination (Tpiaha) Test by a Micromethod

“…Four of them (Nos. 1, 8, 9 and 11) were negative and the others positive in the TPHA test (Tomizawa et al, 1969), while four sera (Nos. 1,6,8 and 11) were nonreactive in the RPR card test (Portnoy, 1963).…”

“…Antigen : Cells of Treponema pallidum Nichols strain were prepared in the same manner as described for TPHA (Tomizawa et al, 1969). The organisms were washed once with GVB++ and suspended in the same buffer to a concentration of 2-3x 107 cells per ml.…”

Risk factors of cervical intraepithelial lesion in Douala-Cameroon: Implications of Herpes Simplex Virus Type 2, Chlamydia Trachomatis and Treponema Pallidum

“…Each sample was analyzed by indirect blocking ELISA protocol (17). The detection of antibodies to T. Pallidum was performed by the method of heamagglutination (18). Commercial Kit TPHA (Treponema pallidum heamagglutination assay) of Biomagrehb laboratory was used for this purpose.…”