Treponema pallidum haemagglutination test for syphilis. Evaluation of a modified micro-method.

Johnston, Nancy

doi:10.1136/sti.48.6.474

Cited by 30 publications

(23 citation statements)

References 11 publications

(10 reference statements)

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“…9 In the present study, 100 patient's blood samples in different stages of syphilis were subjected to VDRL, RPR and Micro-TPHA tests. Comparison of results of 3 tests showed that out of 100 sera, 85 sera were VDRL reactive and RPR test was in 84 sera.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Micro-Tpha, VDRL and RPR Tests in the Serodiagnosis of Syphilis

T¹,

S²,

Gurram³

et al. 2016

jemds

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Syphilis is one of the most fascinating diseases of humans. Widespread use of antibiotics now has reduced the incidence of early syphilis and recognized new cases of late syphilis have decreased. Serological tests are the basis for the diagnosis of latent syphilis. They vary in their sensitivity and specificity. While non-treponemal tests (VDRL and RPR) are most frequently used as screening procedures, treponemal tests (FTA-ABS and TPHA) are considered to be highly sensitive and specific. MATERIALS AND METHODSIn this study 100 serum samples collected from the patients attending STD Department, King George Hospital, Visakhapatnam. Samples were subjected to VDRL, RPR and Micro-TPHA tests to evaluate the sensitivity and specificity of Micro-TPHA test in patients with various stages of syphilis and to evaluate the efficacy of Micro-TPHA to confirm the diagnosis of syphilis and to rule out biological false positive reactions or false negative reactions of VDRL tests. RESULTSThree tests (VDRL, RPR and Micro-TPHA) were applied to all 100 patients. Among them 85 were VDRL reactive, 84 were RPR positive and 89 were TPHA positive. In them males were more in number, in 3 tests with respect to age, (21-30) age group patients showed predominance. Out of 100 patients, 81 sera were positive for all three tests and one serum sample was VDRL reactive, but RPR and TPHA were non-reactive. This may be a false positive reaction. The following tables showed the sensitivity and specificity of each test and also the sensitivity of each test in various stages of syphilis. CONCLUSIONIt is concluded as by performing TPHA test, false positive reactivity of VDRL test could be detected. Even in the cases of treated primary and secondary syphilis, TPHA was reactive. The lipoidal antigen tests primarily reflect disease activity and serial quantitative performance of these tests used to measure the treatment response.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Micro-Tpha, VDRL and RPR Tests in the Serodiagnosis of Syphilis

T¹,

S²,

Gurram³

et al. 2016

jemds

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show abstract

“…The FTA-ABS test was performed as described by Hunter et al (1964) and the TPHA test as described by Johnston (1972).…”

Section: Serologymentioning

confidence: 99%

False-positive test results for syphilis in relatives of a patient with systemic lupus erythematosus.

Jessop¹,

Botha²

1979

Sexually Transmitted Infections

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“…In applied as a confirmatory test instead of, or in addition to, the FTA-ABS because of the former's sensitivity and specificity. This method has been recommended for verification purposes by several authors, including Johnston (1972), Kiraly andPrerau (1974), Vaisman et al (1974), Rudolph (1976), andLesinski et al (1977). Both the FTA-ABS and TPHA tests are known to possess considerable but not absolute specificity and may give non-specific positive results in comparatively few instances.…”

Section: Introductionmentioning

confidence: 99%

Specificity of the FTA-ABS and TPHA tests during pregnancy.

Manikowska-Lesińska¹,

Linda²,

Zájac³

1978

Sexually Transmitted Infections

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SUMMARY In order to determine whether pregnancy influences the specificity of the fluorescent treponemal antibody absorption (FTA-ABS) and Treponema pallidum haemagglutination assay (TPHA) tests, these tests, together with the quantitative fluorescent treponemal antibody (FTA) and Venereal Disease Research Laboratory (VDRL) tests, were carried out simultaneously on 2000 pregnant women who attended for compulsory prenatal screening. In only one patient (0 05 % of the total investigated) was a positive result to the TPHA test obtained, the specificity of which it was impossible to confirm. The FTA-ABS test gave a weakly reactive result of the borderline type but this was found to be non-specific in only four (0-2 %) patients. The results of our investigations showed that the specificity of the FTA-ABS and the TPHA tests performed on pregnant women did not differ from the specificity of these tests when carried out on other population groups.

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Treponema pallidum haemagglutination test for syphilis. Evaluation of a modified micro-method.

Cited by 30 publications

References 11 publications

Evaluation of Micro-Tpha, VDRL and RPR Tests in the Serodiagnosis of Syphilis

Evaluation of Micro-Tpha, VDRL and RPR Tests in the Serodiagnosis of Syphilis

False-positive test results for syphilis in relatives of a patient with systemic lupus erythematosus.

Specificity of the FTA-ABS and TPHA tests during pregnancy.

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