Usefulness of proliferative activity, DNA ploidy pattern and p53 products as diagnostic adjuncts in colorectal adenomas and intramucosal carcinomas

Sugai, Tamotsu; Nakamura, Shinichi; Habano, Wataru; Uesugi, Noriyuki; Sato, Hajime; Yoshida, Toru; Orii, Seishi

doi:10.1046/j.1440-1827.1999.00914.x

Cited by 11 publications

(16 citation statements)

References 22 publications

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“…In previous studies, p53 overexpression, which reflects mutations in p53, has been shown to be associated with aggressive clinical course in several types of cancer, including OPSCC, gastric cancer, and colorectal cancer (20,40,41). In the present study, p53 was frequently found to have a high PR (almost equivalent to overexpression) in HPV-negative OPSCC compared with that in HPV-positive OPSCC.…”

Section: Discussionsupporting

confidence: 58%

Expression of cell cycle-related proteins in oropharyngeal squamous cell carcinoma based on human papilloma virus status

et al. 2017

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The molecular association between human papilloma virus (HPV) and cell cycle-related (CCR) proteins is not fully understood in oropharyngeal squamous cell carcinoma (OPSCC). Herein, we examined the expression levels of CCRs in OPSCCs based on HPV status. In situ hybridization (ISH)- and polymerase chain reaction (PCR)-based assays were used to detect HPV status in 98 OPSCCs, and CCRs were detected by immunostaining. Of 98 tumors, 47 had HPV-positive tumors by either ISH- or PCR-based assays. Concordance analysis between ISH- and PCR-based tests showed a good agreement in OPSCC. Expression of p16 was moderate, consistent with HPV positivity as determined by ISH-based analysis. High p53-, phospho (p)-Rb-, SKP2-, cyclin D1-, and p-c-myc-positive rates were correlated with HPV-negative tumors, whereas high p16- and p27-positive rates were associated with HPV-positive tumors. The positive rates for p21 and cyclin A did not differ between HPV-positive and HPV-negative tumors. Finally, Ki-67 positivity was commonly observed for both types of OPSCCs. Although expression of p16 is thought to be a marker of HPV infection, ISH- or PCR-based tests should be used for HPV detection. In addition, our results regarding CCRs may be helpful for understanding the carcinogenesis of OPSCC.

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Section: Discussionsupporting

confidence: 58%

Expression of cell cycle-related proteins in oropharyngeal squamous cell carcinoma based on human papilloma virus status

et al. 2017

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“…Due to such heterogeneity, the cellular ploidy pattern seems to be dependent on the place where tumour material is extracted. Some authors claim that DNA analysis from one segment only is not sufficient (20,23,24). Rognum (15) observed that the more segments of the tumour that were examined, the more frequently aneuploid tumours were recognized.…”

Section: Discussionmentioning

confidence: 99%

“…Sugai (24) showed that among in 146 of 164 (89%) cases of colorectal carcinoma, at least one of six segments was aneuploid. He concluded that if a single segment is examined, aneuploid clones may be masked by assuming a CV that is too high, which results in false interpretation and qualifying such a clone to be diploid.…”

Section: Discussionmentioning

confidence: 99%

DNA Ploidy in the Treatment of Large Bowel Adenocarcinoma: A Follow up Study

Gawrychowski¹,

Tyczyński²,

Danik³

et al. 2008

Polish Journal of Surgery

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The aim of the study was to look for unfavourable prognostic features in colorectal cancer patients after their surgical treatment as well as to evaluate the prognostic value of cellular ploidy and proliferative activity. Material and methods. A group of 71 colorectal cancer patients discharged from the hospital after surgical treatment in the years 1995-2000 was studied. The examined material was acquired from paraffin blocks of tumour segments. After histopathological verification, the tumour segments recovered from paraffin blocks were used for cytofluorometric analysis of cellular ploidy according to the Hedley method. Results. Diploid tumours were found in 45 of 71 (63.4%) colorectal cancer patients. No significant relationship between DNA ploidy and sex, age, complications, inherited susceptibility to a disease, tumour magnitude, grade of histological malignancy, or grade of clinical progression was observed. After colorectal cancer surgery, the probability of five-and ten-year survival was 44.4% and 37.1%, respectively, for the patients with diploid tumours (DI=1.0), and 38.5% and 9%, respectively, for the patients with aneuploid tumours (DI¹1.0). These differences were not statistically significant (p=0.120). Conclusions. 1. Classical clinicopathologic factors are still the best prognostic criteria for the evaluation of the future results of colorectal cancer patients' surgical treatment. 2. Determination of cellular ploidy and proliferative activity of colonic adenocarcinoma cannot increase the ability to predict prognosis based on surgical treatment . Key words: large bowel adenocarcinoma, DNA ploidy, proliferative activity of large bowel adenocarcinoma Surgical treatment, with possible adjuvant therapy depending on the progression of disease, gives large bowel cancer patients the greatest possibility for recovery (1). However, as the evaluation of clinicopathologic criteria is often subjective and limited, data on prognosis are not objective, and if surgical treatment of large bowel cancer patients is unsuccessful, it is necessary to search for other unknown factors to identify the patients with a risk of a recurrence.The purpose of this study is the following: -to look for unfavourable prognostic features in large bowel cancer patients after their surgical treatment, -to evaluate the prognostic value of cellular ploidy and proliferative activity of large bowel adenocarcinoma.

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“…However, the possibility that polypoid cancer without any adenomatous region might derive from de novo cancer cannot be ruled out. In contrast, DNA aneuploidy is also closely associated with development of colorectal tumors 8–10 . In fact, DNA aneuploidy is thought to be an important risk factor for the increased malignant potential of colorectal adenomas 8–10 .…”

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confidence: 99%

“…In contrast, DNA aneuploidy is also closely associated with development of colorectal tumors 8–10 . In fact, DNA aneuploidy is thought to be an important risk factor for the increased malignant potential of colorectal adenomas 8–10 . Therefore, the association of DNA ploidy pattern with polypoid tumors is not yet fully understood.…”

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confidence: 99%

Evolution of DNA ploidy state and DNA index in colorectal adenomas and carcinomas using the crypt isolation technique: New hypothesis in colorectal tumorigenesis

Sugai

Uesugi

Nakamura

et al. 2003

Pathology International

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The evolution of DNA diploid, aneuploid and multiploid (diploid and aneuploid) states that represent DNA types that are independent of genetic alterations in colorectal tumors were examined. Changes in the DNA index (DI) accompanying tumor development from adenoma to carcinoma were assessed. In colorectal adenomas and early cancers, the DNA was diploid or multiploid. A pure aneuploid state was observed in advanced carcinomas only, whereas the aneuploid DI values of adenomas were characterized by two distinct peaks. The DI values for the carcinomas were randomly distributed. However, in advanced carcinomas, aneuploid carcinomas tended to have lower DI whereas aneuploid populations within multiploid carcinomas tended to have higher DI. Early cancers were subdivided into two groups: a cancer region associated with an adenomatous region (group A tumors) and a cancer region that exhibited an absence of or a very limited adenomatous region (group B tumors). Group A tumor DI were lower than group B. It is suggested that low DI adenomas might transform into group A tumors, which consequently progress to advanced aneuploid carcinomas. In addition, group B tumors might derive predominantly from high DI adenomas or from group A tumors by high DI evolution, and might progress into advanced multiploid carcinomas. Therefore, the evolution of the DNA index might play an important role in the development of colorectal tumors.

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Usefulness of proliferative activity, DNA ploidy pattern and p53 products as diagnostic adjuncts in colorectal adenomas and intramucosal carcinomas

Cited by 11 publications

References 22 publications

Expression of cell cycle-related proteins in oropharyngeal squamous cell carcinoma based on human papilloma virus status

Expression of cell cycle-related proteins in oropharyngeal squamous cell carcinoma based on human papilloma virus status

DNA Ploidy in the Treatment of Large Bowel Adenocarcinoma: A Follow up Study

Evolution of DNA ploidy state and DNA index in colorectal adenomas and carcinomas using the crypt isolation technique: New hypothesis in colorectal tumorigenesis

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