Usefulness of IS6110-based restriction fragment length polymorphism analysis in fingerprinting of Mycobacterium tuberculosis isolates in North India

Mathuria, Jitendra Prasad; Anupurba, Shampa

doi:10.1016/j.ijmyco.2016.10.041

Cited by 3 publications

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“…MTB molecular markers promoted the development of reproducible genotyping methods [4], including insertion sequence 6110 (IS6110), restriction fragment length polymorphism (RFLP) typing [5], spacer oligonucleotide typing (spoligotyping) [6], single nucleotide polymorphism analysis [7],mycobacterial interspersed repetitive unit variable number tandem repeats (MIRU-VNTRs) assessment [8], large sequence polymorphism (LSP) typing [9], and genome [10] sequence analysis. IS6110-RFLP has been the gold standard for genotyping MTB since 1993, but this procedure is time consuming, technically demanding and labour intensive.…”

Section: Introductionmentioning

confidence: 99%

Role of MIRU-VNTR and spoligotyping in assessing the genetic diversity of Mycobacterium tuberculosis in Henan Province, China

et al. 2018

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BackgroundTuberculosis remains a serious threat to human health as an infectious disease in China. Henan, a most populated province in China, has a high incidence of tuberculosis (TB). Though the genetic diversity of Mycobacterium tuberculosis (MTB) has been investigated in many regions, there have been only a few studies on the molecular characteristics and drug resistance phenotypes in Henan. This is the first study on the genetic profile of MTB from Henan.MethodsA total of 668 MTB isolates from various areas were genotyped with spoligotyping and 26-locus MIRU-VNTR (classical 24-locus MIRU-VNTR and 2 other loci). The association between TB spoligotype signatures and drug-resistant profiles was analysed.ResultsOur data revealed that MTB isolates circulating in Henan had a high degree of genetic variation. The Beijing family was the most predominant genotype (83.53%,n = 558), and the typical Beijing type(ST1) was the major sublineage (81.73%,n = 546). In total,668 isolates were divided into 567 different types, forming 38 clusters (2–15 isolates per cluster), and 529 unique types by 26-locus MIRU-VNTR analysis. There was no correlation between the Beijing family and gender, age at diagnosis or treatment history, whereas the Beijing family was significantly associated with all four first-line drug resistance and multidrug-resistant phenotypes. For these samples, 15 of 26 MIRU-VNTR loci had high or moderate discriminatory power according to the Hunter-Gaston discriminatory index. A combination of the 10 most polymorphic loci had similar discriminatory power as the 26-locus set.ConclusionThe Beijing genotype is the most prevalent family. Ten-locus MIRU-VNTR in combination with spoligotyping can efficiently classify the molecular type of MTB in Henan Province.Electronic supplementary materialThe online version of this article (10.1186/s12879-018-3351-y) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Role of MIRU-VNTR and spoligotyping in assessing the genetic diversity of Mycobacterium tuberculosis in Henan Province, China

et al. 2018

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“…Though many have raised a question of using this IS6110 marker in India due to its presence of either in low copy or its complete absence from the genome of MTB the study from North India have proved the utility of IS6110-RFLP a promising genotyping method with good discriminatory power to differentiate strains in MTB isolates in India. 16 This insertion element IS6110 is present in multiple copies and can range from 0-25. 12 Among the 100 isolates that were tested by RFLP in this study, with a male gender being higher than the female in the ratio of 11:9 where globally more men are seen to be diagnosed with TB than women with a ratio of approximately 60:40 between men and women due to various reasons.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the molecular diversity of Mycobacterium tuberculosis isolates from patients attending central referral hospital, East Sikkim based on the IS6110 element

Tewari

Adhikari²,

Khandelwal³

et al. 2022

Int J Res Med Sci

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Background: Using the marker IS6110, which is rated the gold standard for molecular research of Mycobacterium tuberculosis complex (MTB),this study seeks to determine the type of circulating strain of M tuberculosis complex (MTB). MTB genotype was determined from clinical samples of patients exhibiting pulmonary and extrapulmonary tuberculosis at the Central Referral Hospital, SMIMS, Sikkim.Methods: The PCR using IS6110 and 38kDa markers were used for identifying the isolates of M tuberculosis complex in LJ slants and restriction fragment length polymorphism methodology for identifying number of copies of IS6110 had been used to classify 100 M tuberculosis isolates (RFLP-PCR).Results: IS6110 comprised a number of bands ranging between 0 to 2, with the majority of isolates having a single copy, followed by two copies, and a few isolates bearing no copy.Conclusions: This study showed a low copy of IS6110 the most predominant circulating strain in the community of Sikkim with high frequency of 85% of single copy of IS6110.

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Advances in the Diagnosis of Mycobacterium tuberculosis Infection

Silva

Pina

Rêgo

et al. 2018

Advanced Techniques in Diagnostic Microbiology

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Usefulness of IS6110-based restriction fragment length polymorphism analysis in fingerprinting of Mycobacterium tuberculosis isolates in North India

Cited by 3 publications

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Role of MIRU-VNTR and spoligotyping in assessing the genetic diversity of Mycobacterium tuberculosis in Henan Province, China

Role of MIRU-VNTR and spoligotyping in assessing the genetic diversity of Mycobacterium tuberculosis in Henan Province, China

Analysis of the molecular diversity of Mycobacterium tuberculosis isolates from patients attending central referral hospital, East Sikkim based on the IS6110 element

Advances in the Diagnosis of Mycobacterium tuberculosis Infection

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