Advances in the Diagnosis of Mycobacterium tuberculosis Infection

Silva, Duanne Alves da; Pina, Lucindo Cardoso de; Rêgo, Amanda M.; Ferreira, Nicole Victor; Redner, Paulo; Antunes, L. Caetano M.

doi:10.1007/978-3-319-95111-9_4

Cited by 1 publication

(3 citation statements)

References 93 publications

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“…A method employed by Cepheid in Xpert MTB/RIF tests not only allows for early tuberculosis diagnosis (2 h), but also indicates resistance of the strain to rifampicin (first line antituberculosis drug). The WHO, however, recommends caution and points to the need to confirm such a result with a microbial culture and a Ziehl-Neelsen stained sputum microscopic slide [21].…”

Section: Nucleic Acid Amplification Tests (Naats)mentioning

confidence: 99%

“…16S rRNA is used for very accurate species identification and is suitable for taxonomic purposes by comparing the analysed sequences with those from the database (such as SILVA, RDP, GreenGenes or NCBI) [23]. The greatest benefit of sequencing is the ability to detect even the most atypical microorganisms from a clinical specimen, which is helpful in the case of sepsis or meningitis, and can alsobe applied in the diagnosis of tuberculosis [21]. 16S rRNA sequencing enabled the analysis of the pulmonary system microbiome complexity [22].…”

Section: Next Generation Sequencing (Ngs)mentioning

confidence: 99%

“…MALDI is also a valuable method in the case of Mycobacterium tuberculosis infection. An active disease can be diagnosed when CFP-10 and ESAT-6 antigenic peptides secreted by virulent M. tuberculosis strains are detected in a blood sample [21].…”

Section: Matrix-assisted Laser Desorption/ionisation -Time Of Flight Mass Spectrometry (Maldi-tof Ms)mentioning

confidence: 99%

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Advanced methods of bacteriological identification in a clinical microbiology laboratory

Żukowska¹

2021

J Pre Clin Clin Res.

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Introduction and objective. Conventional, culture-based methods of bacterial identification and drug-susceptibility testing are considered the gold standard in medical microbiology. In recent years, classical microbiological methods have been supplemented with modern analytical and molecular methods. The aim of the review was to discusses the methods which have been permanently adapted to bacteriological microbiological diagnostics. Abbreviated description of the state of knowledge. Currently, PCR, as well as other nucleic acid amplification tests and sequencing techniques, are part of the standard repertoire of microbiological diagnostics. With regard to the quality and speed of pathogen identification, the introduction of mass spectrometry techniques into routine microbiological diagnostics work-up has been revolutionary. Within a short time in many laboratories, Matrix-Assisted Laser Desorption/Ionisation -Time of Flight Mass Spectrometry (MALDI TOF MS) systems have almost completely replaced conventional biochemical pathogen identification. Conclusions. Microbiological diagnostics is an indispensable element of a targeted therapy. The techniques used in the laboratory depend primarily on the laboratory's apparatus, the costs of the analysis, as well as the sensitivity and specificity of a method. However, regardless of the culture-based methods universality, advanced techniques have permanently established themselves in diagnostics. Confident information about the detected organism and treatment possibilities in a combination with the clinical context are conducive to successful therapy. Although modern methods still require validation and close collaboration between clinicians, microbiologists and bioinformaticians, these methods, once deemed to be the future, have already arrived.

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Section: Nucleic Acid Amplification Tests (Naats)mentioning

confidence: 99%