Usefulness of GATA-3 as a marker of seminal epithelium in prostate biopsies

Ortiz-Rey, J.A.; Fuente, D. Chantada-de la; Peteiro-Cancelo, M.A.; María, Carolina; Miguel-Fraile, María Pilar San

doi:10.1016/j.acuroe.2017.08.009

Cited by 3 publications

(3 citation statements)

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“…The IHC profile of lining cells shows positivity for AE1/AE3, CK7, p63, CA‐125 and PAX 8, and negativity for PSA, CK20, CDX‐2 and AMACR (Bostwick, ). GATA3 expression has been described in one of the recent reports (Ortiz‐Rey, Chantada‐de la Fuente, Peteiro‐Cancelo, Gómez‐de María, & San Miguel‐Fraile, ) in the normal seminal vesicle epithelium (although no reports in seminal vesicle adenocarcinoma, probably due to its rarity). This normal histology and IHC profile should be kept in mind while analysing a case of seminal vesicle lesion.…”

Section: Discussionmentioning

confidence: 99%

Primary seminal vesicle adenocarcinoma: A case report of rare entity and discussion of its differential diagnosis using immunohistochemical approach for the core biopsy specimen

et al. 2020

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Primary seminal vesicle adenocarcinoma is a rare malignancy of the male genito‐urinary system with only a few confirmed reported cases. Initial tissue diagnostic modality is often a core biopsy specimen. Here, we report this rare entity in a 50‐year‐old male, highlighting the histomorphological and immunohistochemical approach to the core biopsy specimen of the seminal vesicle mass. The patient presented with a history of haematospermia and gross haematuria for one year, and radiological workup was found to have a right seminal vesicle mass. A trans‐rectal ultrasound guided core biopsy revealed a tumourous lesion with a predominant papillary architecture and cytological features of neoplasia. Based on positivity for CK7, PAX‐8 and CA‐125, and Ki‐67 index of 30%–40% and negativity for PSA, AMACR, CK20, CDX‐2, p63, GATA3, WT1 and calretinin, a diagnosis of primary seminal vesicle adenocarcinoma was offered. The diagnosis was also confirmed on the surgically resected specimen. This case depicts the approach of a pathologist to diagnose this rare entity on the core biopsy specimen and the possible differential diagnoses one must consider.

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Section: Discussionmentioning

confidence: 99%

Primary seminal vesicle adenocarcinoma: A case report of rare entity and discussion of its differential diagnosis using immunohistochemical approach for the core biopsy specimen

et al. 2020

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“…GATA3 has been consolidated as biomarker for diagnosis of several neoplasms, including breast cancer, urothelial tumors, germ cell neoplasms and mesotheliomas [9,10,11]. Moreover, GATA3 gene expression has been associated in the prognosis of PTCL/NOS.…”

Section: Introductionmentioning

confidence: 99%

<strong><em>GATA 3</em> Gene Overexpression Is A Biomarker of Adverse Prognosis for Peripheral T-Cell Lymphoma, Not Otherwise Specified and for ALK-Negative Anaplastic Large Cell Lymphoma: An Exploratory Analysis of 80 Latin American Cases of nodal PTCLs</strong>

Lage¹,

Brito²,

Levy³

et al. 2020

Preprint

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Background: Nodal peripheral T-cell lymphomas (nPTCLs) encompass a heterogeneous group of mature and aggressive lymphoid malignancies, including peripheral T-cell lymphoma, not otherwise specified (PTCL/NOS), angioimmunoblastic T-cell lymphoma (AITL) and anaplastic large cell lymphoma (ALCL) ALK-positive and ALK-negative. Their differential diagnosis and prognosis are an issue in the clinical practice. Accurate biomarkers to refine the different subtypes of nPTCLs and to stratify their prognosis are essential to improve their treatment approach. The aim of this study was to test the prognostic impact of GATA3 gene expression, and its capability to discriminate the different subtypes of nPTCLs. Patients and Methods: From 2000 to 2017, 80 patients with nPTCLs were eligible for GATA3 gene expression analysis that was assessed retrospectively by quantitative real time PCR (qRT-PCR) of neoplastic biopsies in Formalin-Fixed Paraffin-Embedded samples (FFPE). Results: Median age was 49 years old (IqR 34-59), 43/80 (53.7%) were male. Median follow-up was 1.72 years. Of them, 36.3% were classified as PTCL/NOS, 31.2% ALK-negative ALCL, 21.2% ALK-positive ALCL and 11.3% AITL. The majority of cases had advanced stage (III/IV). Two-year estimated overall survival (OS) and progression-free survival (PFS) were 52.2% and 39.5%, respectively. The median GATA3 gene expression level was 0.49% (range 0 – 7.07) in all cohort, it was 0.11% for ALK-positive ALCL, 0.46% for ALK-negative ALCL, 0.86% for PTCL/NOS and 0.67% for AITL. The difference of GATA3 gene expression among distinct variants of nPTCLs was statistically significant (p < 0.001). GATA3 gene expression levels ≥ 0.71% discriminate PTCL/NOS from ALK-negative ALCL and AITL with sensitivity of 62% and specificity of 80.3%. GATA3 gene expression levels ≥ median was associated with poor 2-year OS for PTCL/NOS (46.7% x 21.4%, p=0.04) and for ALK-negative ALCL (85.7% x 54.5%, p=0.04). Conclusion: Despite the relative small and heterogeneous group of patients with nPTCLs, GATA3 gene overexpression may be an important biomarker associated with poor prognosis in PTCL/NOS and ALK-negative ALCL. Moreover it may also discriminate different subtypes of nPTCLs. Further studies with larger series of patients should confirm our findings.

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“…14 In addition, certain immunomarkers such as MUC-6, PAX8, and GATA-3 have shown their usefulness in identifying between seminal and prostatic epithelium in prostate needle biopsies for the differential diagnosis with prostatic adenocarcinoma. [15][16][17] Regarding the origin of VSC, histologically, the surface of the wall of the seminal vesicles possesses a pseudostratified columnar epithelium composed of columnar and basal epithelial cells. In some zones, the columnar epithelium typically contains large amounts of lipofuscin pigment.…”

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confidence: 99%

Voided urine cytology in a case of hematospermia

Martínez‐Girón

Martínez-Torre

2019

Diagnostic Cytopathology

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Hematospermia or hemospermia is a clinical condition, which describes the presence of blood in the ejaculate. Although this rare disorder can be worrisome for patients, it is usually benign and selflimited. 1 That said, some patients may require further evaluation by a specialist when considered alongside other factors such as the patient's age (>40 years), recurrent episodes or persistent bleeding, prostate cancer risk or presence of constitutional symptoms. 2 Precise incidences of hematospermia are difficult to identify, as examinations of the semen are not usually made by the affected individuals, and there are few follow-up medical consultations. 3 The causes of hematospermia include the following: infections (bacterial, viral, and parasitic, eg, schistosomiasis); malignancy (prostate, bladder, testicular, seminal vesicles); iatrogenic (certain medications, prostatic massage, needle prostatic biopsy, and urological surgery); trauma (coital, perianal); ductal obstruction (cysts and/or calculi); systemic disorders (hypertension, amyloidosis, haemopathies, cirrhosis, hyperuricemia, among others); behavioral (excessive intercourse or masturbation, prolonged sexual abstinence); vascular (arteriovenous malformations, hemangioma); and idiopathic. 4 An appropriate treatment should be directed at the diagnosed etiology (eg, antibiotics, adequate control of systemic disorders, for example, hypertension, urological surgery, etc.). It is also important to rule out so-called pseudohematospermia in cases of haematuria or a partner's sexual bleeding (condom use can help rule out this possibility in such cases). 5 Hematospermia should also be distinguished from melanospermia, a very uncommon condition that occurs because of metastatic melanoma lesions in certain organs such as the prostate and seminal vesicles. 6 Nevertheless, although it is also a very rare clinical entity, the metastasis in seminal vesicles from a melanoma can also be the cause of hematospermia. 7 Herein, we report the case of a healthy 42-year-old male who presented two episodes of hematospermia in the last month. Among the clinical records, we can highlight hypertension, hyperuricemia, and vasectomy performed 3 years ago. A digital rectal examination was normal. Blood analysis, voided urine cytology, urine sediment, fluid seminal analysis, urine and seminal cultures were carried out. A blood analysis was unremarkable (including prostate-specific antigen [PSA] levels) except for the existence of hyperuricemia (7.9 mg/dL). The rest of the exams of the urine sediment, fluid seminal analysis, cultures for bacteria (included mycobacteria), chlamydia, trichomonas, and fungi were normal. Only in the urine cytology, the presence of two cellular groups of discohesive cells showing enlargement of nuclear size, anisokaryosis, hyperchromasia, and poorly defined scant cytoplasms with a high N/C ratio (Figure 1) were F I G U R E 1 Voided urine cytology showing seminal vesicle cells. Marked atypia, intracytoplasmic lipofuscin pigment, and some spermatozoa are visible (...

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Usefulness of GATA-3 as a marker of seminal epithelium in prostate biopsies

Cited by 3 publications

References 20 publications

Primary seminal vesicle adenocarcinoma: A case report of rare entity and discussion of its differential diagnosis using immunohistochemical approach for the core biopsy specimen

Primary seminal vesicle adenocarcinoma: A case report of rare entity and discussion of its differential diagnosis using immunohistochemical approach for the core biopsy specimen

<strong><em>GATA 3</em> Gene Overexpression Is A Biomarker of Adverse Prognosis for Peripheral T-Cell Lymphoma, Not Otherwise Specified and for ALK-Negative Anaplastic Large Cell Lymphoma: An Exploratory Analysis of 80 Latin American Cases of nodal PTCLs</strong>

Voided urine cytology in a case of hematospermia

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