Usefulness of Elevated Urine Neopterin Levels in Assessing Cardiac Dysfunction and Exercise Ventilation Inefficiency in Patients With Chronic Systolic Heart Failure

Saturation diving allows divers to reduce the risk of decompression sickness while working at depth for prolonged periods but may increase reactive oxygen species (ROS) production. Such modifications can affect endothelial function by exacerbating oxidative stress. This study investigated the effects of saturation diving on oxidative stress damage. Redox status was evaluated through: ROS production; total antioxidant capacity (TAC); nitric oxide metabolites (NOx); nitrotyrosine (3-NT); and lipid peroxidation (8-iso-PGF2α) assessment. Creatinine and neopterin were analyzed as markers of renal function and damage. Measurements were performed on saliva and urine samples obtained at four time points: pre; deep; post; and 24 h post. Four divers were included in the study. After the saturation dive (post), significant (p < 0.05) increases in ROS (0.12 ± 0.03 vs. 0.36 ± 0.06 µmol.min−1), TAC (1.88 ± 0.03 vs. 2.01 ± 0.08 mM), NOx (207.0 ± 103.3 vs. 441.8 ± 97.3 µM), 3-NT (43.32 ± 18.03 vs. 18.64 ± 7.45 nM·L−1), and 8-iso-PGF2α (249.7 ± 45.1 vs. 371.9 ± 54.9 pg·mg−1 creatinine) were detected. Markers of renal damage were increased as well after the end of the saturation dive (creatinine 0.54 ± 0.22 vs. 2.72 ± 1.12 g-L−1; neopterin 73.3 ± 27.9 vs. 174.3 ± 20.53 μmol·mol−1 creatinine). These results could ameliorate commercial or military diving protocols or improve the understanding of symptoms caused by oxygen level elevation.

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“…The levels of neopterin and creatinine can increase during systemic oxidative stress [ 13 , 14 , 40 , 41 ].…”

Section: Discussionmentioning

confidence: 99%

Change in Oxidative Stress Biomarkers During 30 Days in Saturation Dive: A Pilot Study

Mrakic‐Sposta

Vezzoli

D'Alessandro

et al. 2020

IJERPH

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“…An increase in systemic inflammation, besides the markers of oxidative stress, can also be associated to altered biochemical parameters measured in urine and in particular to Neopterin concentration level, that has previously been shown to indicate an enhancement of immune response [ 49 ]. Recently, high Neopterin production has been associated with immune cellular activation and increased production of OxS [ 50 ]. Therefore, throughout Neopterin assessment, not only the extent of cellular immune activation, but also the extent of OxS can be estimated [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Mountain Ultra-Marathon Running on ROS Production and Oxidative Damage by Micro-Invasive Analytic Techniques

et al. 2015

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PurposeAiming to gain a detailed insight into the physiological mechanisms involved under extreme conditions, a group of experienced ultra-marathon runners, performing the mountain Tor des Géants® ultra-marathon: 330 km trail-run in Valle d’Aosta, 24000 m of positive and negative elevation changes, was monitored. ROS production rate, antioxidant capacity, oxidative damage and inflammation markers were assessed, adopting micro-invasive analytic techniques.MethodsForty-six male athletes (45.04±8.75 yr, 72.6±8.4 kg, 1.76±0.05 m) were tested. Capillary blood and urine were collected before (Pre-), in the middle (Middle-) and immediately after (Post-) Race. Samples were analyzed for: Reactive Oxygen Species (ROS) production by Electron Paramagnetic Resonance; Antioxidant Capacity by Electrochemistry; oxidative damage (8-hydroxy-2-deoxy Guanosine: 8-OH-dG; 8-isoprostane: 8-isoPGF2α) and nitric oxide metabolites by enzymatic assays; inflammatory biomarkers (plasma and urine interleukin-6: IL-6-P and IL-6-U) by enzyme-linked immunosorbent assays (ELISA); Creatinine and Neopterin by HPLC, hematologic (lactate, glucose and hematocrit) and urine parameters by standard analyses.ResultsTwenty-five athletes finished the race, while twenty-one dropped out of it. A significant increase (Post-Race vs Pre) of the ROS production rate (2.20±0.27 vs 1.65±0.22 μmol.min-1), oxidative damage biomarkers (8-OH-dG: 6.32±2.38 vs 4.16±1.25 ng.mg-1 Creatinine and 8-isoPGF2α: 1404.0±518.30 vs 822.51±448.91 pg.mg-1Creatinine), inflammatory state (IL-6-P: 66.42±36.92 vs 1.29±0.54 pg.mL-1 and IL-6-U: 1.33±0.56 vs 0.71±0.17 pg.mL1) and lactate production (+190%), associated with a decrease of both antioxidant capacity (-7%) and renal function (i.e. Creatinine level +76%) was found.ConclusionsThe used micro-invasive analytic methods allowed us to perform most of them before, during and immediately after the race directly in the field, by passing the need of storing and transporting samples for further analysis. Considered altogether the investigated variables showed up that exhaustive and prolonged exercise not only promotes the generation of ROS but also induces oxidative stress, transient renal impairment and inflammation.

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“…Изучению уровня неоптерина у больных с ХСН посвящены единичные исследования. По данным [11,12] уровень неоптерина в моче и плазме крови у больных с ХСН коррелирует с прогрессированием нарушения функции и структуры ЛЖ. Установлено, что у больных с СНсФВ уровень неоптерина имел корреляционные связи с утяжелением функционального класса ХСН [13].…”

Section: Discussionunclassified

Analysis of inflammatory marker levels in patients with chronic heart failure

Попов

Полунина

Воронина

et al. 2018

Cardiovasc Ther Prev

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Aim.To analyze the levels of fractalkine, neopterin and C-reactive protein (CRP) in patients with chronic heart failure (CHF) depending on the left ventricular ejection fraction (LVEF) and stage of the disease.Material and methods.We examined 340 people (280 patients with CHF) divided into groups depending on LVEF and stage of the disease (stage I-III). The control group consisted of 60 somatically healthy individuals. Levels of neopterin, fractalkine and CRP were indicated with the use of enzymoimmunoassay.Results.Compared with the control group, patients with CHF, regardless of LVEF and the stage of the disease, had hyperproduction of neopterin, fractalkine and CRP. We indicated correlations between the level of neopterin, fractalkine, CRP and Rating Scale of Clinical State (RSCS) scores, as well as with the results of 6-minute walk distance. Compared with patients with CHF IIA, patients with CHF IIB-III and preserved LVEF, had a decrease in levels of fractalkine, neopterin and CRP.Conclusion.Changes in levels of neopterin, fractalkine and CRP were detected in patients with CHF and preserved and reduced LVEF. More significant tendency to hyperproduction was noted in patients with reduced LVEF in the group of patients with CHF IIB-III.

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Usefulness of Elevated Urine Neopterin Levels in Assessing Cardiac Dysfunction and Exercise Ventilation Inefficiency in Patients With Chronic Systolic Heart Failure

Cited by 11 publications

References 27 publications

Change in Oxidative Stress Biomarkers During 30 Days in Saturation Dive: A Pilot Study

Change in Oxidative Stress Biomarkers During 30 Days in Saturation Dive: A Pilot Study

Effects of Mountain Ultra-Marathon Running on ROS Production and Oxidative Damage by Micro-Invasive Analytic Techniques

Analysis of inflammatory marker levels in patients with chronic heart failure

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