Usefulness of Breath Test for Evaluating Pancreatic Exocrine Function Using N-Benzoyl-L-tyrosyl-1-13C-L-alanine Sodium in Non-invasive and Conscious Rats

Uchida, Masayuki; Mogami, Orie

doi:10.1248/bpb.31.785

Cited by 6 publications

(4 citation statements)

References 16 publications

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“…The controversy of anesthetic choice is only partially circumvented in studies of gastrointestinal motility that are performed in the conscious, behaving animal17–21. Commonly, these studies are based upon a single parameter of the entire gastric emptying process such as gastric contractility17,18 or are outcome based measurements such as post-mortem assays for gastric emptying of a meal challenge22–24.…”

Section: Introductionmentioning

confidence: 99%

“…The controversy of anaesthetic choice is only partially circumvented in studies of gastrointestinal motility that are performed in the conscious, behaving animal. [17][18][19][20][21] Commonly, these studies are based upon a single parameter of the entire gastric emptying process such as gastric contractility 17,18 or are outcome based measurements such as postmortem assays for gastric emptying of a meal challenge. [22][23][24] Non-invasive breath tests for gastric emptying utilizing [ 13 C]-isotopeenriched substrates have been validated in a number of species including humans and rodents [25][26][27][28][29] with the added benefit that this technique permits safe, repeatable, measurement of gastric emptying in the same animal.…”

Section: Introductionmentioning

confidence: 99%

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Gastric emptying of enterally administered liquid meal in conscious rats and during sustained anaesthesia

Qualls‐Creekmore

Tong

Holmes

2010

Neurogastroenterology &Amp; Motility

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Gastric motility studies are frequently conducted with anesthetized animal models. Some studies on the same animal species have reported differences in vagal control of the stomach that could not be explained solely by slightly different experimental conditions. A possible limitation in the comparison between similar studies relates to the use of different anesthetic agents. Furthermore, anesthetic effects may also limit generalizations between mechanistic studies of gastric motility and the gastric motility of conscious animals. In the present study, we used the [13C]-breath test following a liquid mixed-nutrient test meal (Ensure®, 1 ml) with the aim to investigate the rate of gastric emptying in animals that were either conscious or anesthetized with either Inactin® or urethane. One week after determining the maximum 13CO2 concentration, time to peak [13C] recovery and gastric half emptying time in control, conscious rats, we repeated the experiment in the same rats anesthetized with Inactin® or urethane. Our data show that Inactin® anesthesia prolonged the time to peak [13C] recovery but did not significantly reduce the maximum 13CO2 concentration nor delay gastric half emptying time. Conversely, urethane anesthesia resulted in a significant slowing of all parameters of gastric emptying as measured by the maximum 13CO2 concentration, time to peak [13C] recovery and half emptying time. Our data indicate that Inactin® anesthesia does not significantly affect gastric emptying while urethane anesthesia profoundly impairs gastric emptying. We suggest that Inactin®, not urethane, is the more suitable anesthetic for gastrointestinal research.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gastric emptying of enterally administered liquid meal in conscious rats and during sustained anaesthesia

Qualls‐Creekmore

Tong

Holmes

2010

Neurogastroenterology &Amp; Motility

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“…On the other hand, in basic research we developed a simple and non-invasive breath test system for evaluating gastric emptying and pancreatic exocrine secretion in conscious rats [6][7][8]. Recently, we also reported a non-invasive and feasible method for the evaluation of the rat gastrocecal transit time by using lactose-[ 13 C]ureide [9].…”

Section: Introductionmentioning

confidence: 99%

Effect of atropine sulfate on gastric emptying and gastrocecal transit time evaluated by using the [1- ¹³ C]acetic acid and lactose-[ ¹³ C]ureide breath test in conscious rats

Uchida¹,

Yoshida²,

Shimizu³

2009

J. Breath Res.

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Breath tests have been used to investigate many physiological functions. Recently, we have developed a breath test system by using a non-invasive technique in conscious rats. In this study, we investigated the effect of atropine sulfate on the gastric emptying and gastrocecal transit time using [1-(13)C]acetic acid and lactose-[(13)C]ureide, respectively. Gastric emptying was significantly delayed by atropine sulfate in a dose-dependent manner (0.03-0.3 mg kg(-1)), but the effects of 0.1 and 0.3 mg kg(-1) were almost equal. C(max) and T(max) values were also significantly and dose-dependently decreased and delayed, respectively. However, AUC(120 min) values were not significantly different for the three doses of atropine sulfate. This suggests that although atropine sulfate slows the emptying of the gastric content, it is excreted eventually to the same content as the control. The gastrocecal transit time was significantly delayed by atropine sulfate at a dose of 1 mg kg(-1), but not at a dose of 0.1 mg kg(-1). These findings demonstrate that the breath tests can evaluate the effect of atropine sulfate on the gastric emptying and gastrocecal transit time. The effective dose of atropine sulfate may be different for gastric emptying and gastrocecal transit time.

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“…In basic research, we have already reported a simple and non-invasive breath test system for evaluating gastric emptying and pancreatic exocrine secretion in conscious rats (Uchida et al, 2005;Uchida et al, 2007;Uchida et al, 2008;Matsumoto et al, 2008;Uchida et al, 2011). Moreover, we have also established a non-invasive and feasible method for the evaluation of rat gastrocecal transit time by using lactose-[ 13 C] ureide (Uchida et al, 2009a) and the effect of atropine sulfate which significantly delayed the gatsrocecal transit time (Uchida et al, 2009b).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous measurement of gastric emptying and gastrocecal transit times in conscious rats using a breath test after ingestion of [1-<sup>13</sup>C] acetic acid and lactose-[<sup>13</sup>C] ureide

Uchida

Yoshida-Iwasawa

2012

J. Smooth Muscle Res.

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This study reports a method for the evaluation of both gastric emptying and gastrocecal transit times in rats simultaneously by using the same breath testing system measuring equipment. Male rats were used after fasting. Gastric emptying and gastrocecal transit time were evaluated by using [1-13 C] acetic acid (8 mg/kg) and lactose-[ 13 C] ureide (60 mg/kg), respectively. A mixture of both 13 C-labelled compounds dissolved in Racol (liquid nutrient formula) was administered orally. The level of 13 CO 2 in the expired air was measured using an infrared spectrometer at appropriate intervals for a period of 420 min. The level of 13 CO 2 in the expired air from [1-13 C] acetic acid increased with time and peaked at about 30 min before decreasing, while that from lactose-[ 13 C] ureide increased after about 180 min. The time taken to reach the maximum value of gastric emptying (Tmax) was 27.5 ± 0.9 min. Gastrocecal transit time was 180 ± 11.5 min, which was calculated as the time before the 13 CO 2 value increased again. These results accorded with the results of gastric emptying and gastrocecal transit time evaluated by using each 13 C-labelled compound separately. These results demonstrate that this method is useful for the simultaneous evaluation of gastric emptying and gastrocecal transit times in rats.

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Usefulness of Breath Test for Evaluating Pancreatic Exocrine Function Using N-Benzoyl-L-tyrosyl-1-13C-L-alanine Sodium in Non-invasive and Conscious Rats

Cited by 6 publications

References 16 publications

Gastric emptying of enterally administered liquid meal in conscious rats and during sustained anaesthesia

Gastric emptying of enterally administered liquid meal in conscious rats and during sustained anaesthesia

Effect of atropine sulfate on gastric emptying and gastrocecal transit time evaluated by using the [1- ¹³ C]acetic acid and lactose-[ ¹³ C]ureide breath test in conscious rats

Simultaneous measurement of gastric emptying and gastrocecal transit times in conscious rats using a breath test after ingestion of [1-<sup>13</sup>C] acetic acid and lactose-[<sup>13</sup>C] ureide

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Usefulness of Breath Test for Evaluating Pancreatic Exocrine Function Using N-Benzoyl-L-tyrosyl-1-13C-L-alanine Sodium in Non-invasive and Conscious Rats

Cited by 6 publications

References 16 publications

Gastric emptying of enterally administered liquid meal in conscious rats and during sustained anaesthesia

Gastric emptying of enterally administered liquid meal in conscious rats and during sustained anaesthesia

Effect of atropine sulfate on gastric emptying and gastrocecal transit time evaluated by using the [1- 13 C]acetic acid and lactose-[ 13 C]ureide breath test in conscious rats

Simultaneous measurement of gastric emptying and gastrocecal transit times in conscious rats using a breath test after ingestion of [1-<sup>13</sup>C] acetic acid and lactose-[<sup>13</sup>C] ureide

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Effect of atropine sulfate on gastric emptying and gastrocecal transit time evaluated by using the [1- ¹³ C]acetic acid and lactose-[ ¹³ C]ureide breath test in conscious rats