Useful immunochromatographic assay of calprotectin in gingival crevicular fluid for diagnosis of diseased sites in patients with periodontal diseases

Kido, Jun‐ichi; Murakami, Shinya; Kitamura, Masahiro; Yanagita, Manabu; Tabeta, Koichi; Yamazaki, Kazuhisa; Yoshie, Hiromasa; Watanabe, Hisashi; Izumi, Yuichi; Suda, Reiko; Yamamoto, Masahide; Shiba, Hideki; Fujita, Tsuyoshi; Kurihara, Hidemi; Mizuno, Mitsuharu; Mishima, Akihiro; Kawahara, Nobumasa; Hara, Kazuhiro; Naruishi, Koji; Nagata, Toshihiko

doi:10.1902/jop.2017.170206

Cited by 10 publications

(12 citation statements)

References 39 publications

(51 reference statements)

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“…Recently, a PoC/chair-side device, comparable to PCR-detection, for Porphyromonas gingivalis related to chronic periodontitis detection has been developed [ 28 ]. A chair-side assay for GCF calprotectin has been described [ 29 ]. Calprotectin does not degrade anything [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

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The Ability of Quantitative, Specific, and Sensitive Point-of-Care/Chair-Side Oral Fluid Immunotests for aMMP-8 to Detect Periodontal and Peri-Implant Diseases

et al. 2018

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The analysis of the disease-specific oral and systemic biomarkers in saliva and oral fluids (i.e., mouth rinse, gingival crevicular fluid (GCF), and peri-implantitis fluid (PISF)) is demanding. Several hosts and microbial factors may influence their expression, release, and levels. The type of saliva/oral fluids utilized for the diagnostics affects the analysis. High sensitivity and specificities together with sophisticated methods and techniques are essential for valuable outcome. We describe here recently developed practical, convenient, inexpensive, noninvasive, and quantitative mouth rinse and PISF/GCF/chair-side/point-of-care (PoC) lateral-flow aMMP-8 immunoassays (PerioSafe and ImplantSafe/ORALyser) to detect, predict, and monitor successfully the course, treatment, and prevention of periodontitis and peri-implantitis, respectively. The tests have been independently and successfully validated to differentiate periodontal and peri-implant health and disease in Finland, Germany, Netherland, Sweden, Turkey, Nigeria, Malawi, and USA. The clinical use of salivary/oral fluid biomarkers to identify oral and systemic conditions requires additional studies utilizing these noninvasive screening, diagnostic, and preventive aMMP-8 PoC/chair-side technologies.

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Section: Discussionmentioning

confidence: 99%

“…A chair-side assay for GCF calprotectin has been described [ 29 ]. Calprotectin does not degrade anything [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

The Ability of Quantitative, Specific, and Sensitive Point-of-Care/Chair-Side Oral Fluid Immunotests for aMMP-8 to Detect Periodontal and Peri-Implant Diseases

et al. 2018

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“…S100A9 involved in the regulation of inflammatory processes and immune response [ 29 ]. Calprotectin, S100A8/A9, is the marker for gingivitis and periodontitis [ 30 , 31 ]. Since the main extracellular form of S100A8 and S100A9 is as a heterodimer (S100A8/A9 or calprotectin), the role of calprotectin should have been considered.…”

Section: Discussionmentioning

confidence: 99%

S100A8 and S100A9 in saliva, blood and gingival crevicular fluid for screening established periodontitis: a cross-sectional study

et al. 2021

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Background Periodontitis is one of major oral diseases, which has no consensus on early screening tool. This study aimed to compare the association and screening ability of S100A8 and S100A9 in saliva, blood and gingival crevicular fluid (GCF) for periodontitis status. Methods We recruited 149 community Korean adults, 50 no or initial periodontitis (NIPERIO) and 99 established periodontitis (PERIO). Using clinical attachment loss and a panoramic radiograph, stage II–IV of new classification of periodontitis proposed at 2018 was considered cases as PERIO. Enzyme linked immunosorbent assay kit was used to quantify S100A8 and S100A9. T-test, analysis of covariance, Mann–Whitney test and correlation analysis were applied to compare the relationship of S100A8 and S100A9 in saliva, blood, and GCF for periodontitis. Receiver operating characteristic curve was applied for screening ability. Results Among S100A8 and S100A9 in saliva, blood and GCF, S100A8 in saliva was significantly higher in PERIO than in NIPERIO (p < 0.05). However, S100A8 and S100A9 in GCF were higher in NIPERIO (p < 0.05). The screening ability of salivary S100A8 was 75% for PERIO, while that of GCF S100A8 was 74% for NIPERIO. Salivary S100A8 was positively correlated to blood S100A8 (p < 0.05). Conclusion Salivary S100A8 could be a potential diagnostic marker for established periodontitis and be useful for screening established periodontitis.

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“…Calprotectin is a cytosolic protein released from neutrophils in the early stages of inflammation 15 . It has been found that GCF calprotectin levels were higher in chronic periodontitis patients compared with healthy ones 17‒19 and were significantly reduced after treatment 20‒22 due to decreased inflammatory cell infiltration 15,44 . On the other hand, calprotectin has antimicrobial activity by competing for zinc, which is needed for the growth of bacteria 14 .…”

Section: Discussionmentioning

confidence: 99%

“…In chronic inflammatory diseases such as rheumatoid arthritis, Crohn disease, and cystic fibrosis, calprotectin levels in plasma and synovial fluid are elevated 16 . It has been shown that patients with chronic periodontitis had elevated GCF calprotectin levels 17‒19 and those levels were significantly decreased after treatment 20‒22 . Osteocalcin is a calcium‐binding protein of bone that is synthesized by osteoblasts.…”

Section: Introductionmentioning

confidence: 99%

Full‐mouth disinfection effects on gingival fluid calprotectin, osteocalcin, and N‐telopeptide of Type I collagen in severe periodontitis

Afacan

Çınarcık

Gürkan

et al. 2020

Journal of Periodontology

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Background:To compare the effects of full-mouth disinfection (FMD) and fullmouth ultrasonic debridement (FMUD) on clinical, microbiological and biochemical parameters with conventional quadrant-wise scaling and root planning (Q-SRP) in severe chronic periodontitis. Methods:In the present prospective randomized controlled clinical trial with three parallel arms (#NCT04038801), 60 chronic periodontitis patients were randomly assigned to three study groups by a consecutive number in ascending order: FMD (n = 20), FMUD (n = 20), and Q-SRP (n = 20). All measurements and treatments were performed by the same investigator. At baseline, gingival crevicular fluid (GCF) and subgingival plaque were collected and clinical periodontal parameters were recorded. Ultrasonic debridement was completed within 24 hours in FMD and FMUD groups.Chlorhexidine gluconate was used for FMD. Q-SRP was performed by hand instruments per quadrant at 1-week-intervals. Clinical measurements and sampling were repeated at 1, 3, and 6 months after treatment. Real-time PCR was used for quantitative analysis of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Fusobacterium nucleatum, and total bacteria count. GCF Calprotectin, osteocalcin, and N-telopeptide of type I collagen (NTx) levels were analyzed by ELISA. The changes of GCF biomarker levels after treatment between groups were the primary outcomes. Results:No harm was observed. All treatment strategies resulted in significant improvements in all clinical parameters (P < 0.05), with no significant differences between study groups at all time-points (P ˃ 0.05). Aggregatibacter actinomycetemcomitans was significantly decreased in FMD compared to FMUD and Q-SRP at 6 months (P < 0.05). Although GCF NTx total amounts increased in all groups during 638© 2020 American Academy of Periodontology J Periodontol. 2020;91:638-650. wileyonlinelibrary.com/journal/jper AFACAN ET AL. 639the study period, this increase was less prominent in full-mouth groups at three time points after treatment (P < 0.05). Conclusions:Present results represent the short-term effects. Full-mouth treatment approaches offered limited beneficial effects on microbiological and biochemical parameters over quadrant-wise approach. All three treatment strategies can be recommended in the management of severe chronic periodontitis.

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Useful immunochromatographic assay of calprotectin in gingival crevicular fluid for diagnosis of diseased sites in patients with periodontal diseases

Abstract: Determination of GCF calprotectin using a novel IC chip system is useful for diagnosis of periodontal diseases.

Cited by 10 publications

References 39 publications

The Ability of Quantitative, Specific, and Sensitive Point-of-Care/Chair-Side Oral Fluid Immunotests for aMMP-8 to Detect Periodontal and Peri-Implant Diseases

The Ability of Quantitative, Specific, and Sensitive Point-of-Care/Chair-Side Oral Fluid Immunotests for aMMP-8 to Detect Periodontal and Peri-Implant Diseases

S100A8 and S100A9 in saliva, blood and gingival crevicular fluid for screening established periodontitis: a cross-sectional study

Full‐mouth disinfection effects on gingival fluid calprotectin, osteocalcin, and N‐telopeptide of Type I collagen in severe periodontitis

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