Full‐mouth disinfection effects on gingival fluid calprotectin, osteocalcin, and N‐telopeptide of Type I collagen in severe periodontitis

Abstract

Background:To compare the effects of full-mouth disinfection (FMD) and fullmouth ultrasonic debridement (FMUD) on clinical, microbiological and biochemical parameters with conventional quadrant-wise scaling and root planning (Q-SRP) in severe chronic periodontitis. Methods:In the present prospective randomized controlled clinical trial with three parallel arms (#NCT04038801), 60 chronic periodontitis patients were randomly assigned to three study groups by a consecutive number in ascending order: FMD (n = 20), FMUD (n = 20), and Q-SRP (n = 20). All measurements and treatments were performed by the same investigator. At baseline, gingival crevicular fluid (GCF) and subgingival plaque were collected and clinical periodontal parameters were recorded. Ultrasonic debridement was completed within 24 hours in FMD and FMUD groups.Chlorhexidine gluconate was used for FMD. Q-SRP was performed by hand instruments per quadrant at 1-week-intervals. Clinical measurements and sampling were repeated at 1, 3, and 6 months after treatment. Real-time PCR was used for quantitative analysis of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Fusobacterium nucleatum, and total bacteria count. GCF Calprotectin, osteocalcin, and N-telopeptide of type I collagen (NTx) levels were analyzed by ELISA. The changes of GCF biomarker levels after treatment between groups were the primary outcomes. Results:No harm was observed. All treatment strategies resulted in significant improvements in all clinical parameters (P < 0.05), with no significant differences between study groups at all time-points (P ˃ 0.05). Aggregatibacter actinomycetemcomitans was significantly decreased in FMD compared to FMUD and Q-SRP at 6 months (P < 0.05). Although GCF NTx total amounts increased in all groups during 638© 2020 American Academy of Periodontology J Periodontol. 2020;91:638-650. wileyonlinelibrary.com/journal/jper AFACAN ET AL. 639the study period, this increase was less prominent in full-mouth groups at three time points after treatment (P < 0.05). Conclusions:Present results represent the short-term effects. Full-mouth treatment approaches offered limited beneficial effects on microbiological and biochemical parameters over quadrant-wise approach. All three treatment strategies can be recommended in the management of severe chronic periodontitis.

Is the Microbiome the cause of Irritable Bowel Syndrome and Inflammatory Bowel Disease? Lessons to consider from odontology

“…disease at higher levels compared to healthy controls. 82 Furthermore, a randomised control trial measured Calprotectin levels in the mouth in active periodontitis patients, the levels significantly decreased after periodontal therapy, 83 similar to what is seen in IBD with medications. Lactoferrin is another marker which in both diseases is elevated, also correlating with disease activity, suggesting a similar microbial aetiology.…”

Impact exerted by scaffolds and biomaterials in periodontal bone and tissue regeneration engineering: new challenges and perspectives for disease treatment

“…In this context, acellular dermal matrix (ADM) is a soft tissue transplant derived from decellularized human skin. Devoid of epithelium and cellular components, the ECM functions as a scaffold to enable cellular migration and revascularization from the surrounding host tissues [52][53][54][55]. A potential explanation may be that the ADM is incapable of promoting keratinization of the overlaying epithelia, which appears to be a favorable predictor of gingival margin stability.…”

“…A potential explanation may be that the ADM is incapable of promoting keratinization of the overlaying epithelia, which appears to be a favorable predictor of gingival margin stability. In the existence of a specific amount of keratinized tissue width at baseline, it is possible to achieve comparable root coverage outcomes with ADM as with connective tissues, according to studies [52][53][54][55][56].…”

“…Bacteria (such as Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola) are the bacteria linked with periodontitis, and related host immune responses [10,[53][54][55][56][57]. Even though the current periodontal treatment involves the removal of dental plaque and calculus, the sources and niche of bacteria causing pathological symptoms, the recurrence rate of periodontitis after the initial periodontal treatment is strongly associated with maintenance to prevent secondary infection.…”

The role of the saliva biomarkers in the diagnostics of the oral mucosal cancer

“…В свою очередь, возрастные изменения напрямую влияют на слюнные железы, и угнетение их функций выражается снижением экспрессии биомаркеров в слюне [11]. Аналогичные данные приводят и другие исследователи после определения уровня IL-1β в ротовой жидкости пациентов при пародонтите [12,13].…”