Useful Bioactive Substances from Wastes: Recovery of Trans-Resveratrol from Grapevine Stems

Abstract

The methods for producing natural resveratrol are of big interest because of the many health benefits of this substance and its increasing use in functional foods, food supplements and para-pharmaceutical preparations. Generally, resveratrol is extracted from different natural sources, most of them usually produced for consumption purposes (grapes, nuts). This paper presents a method for recovery of resveratrol from a widely available raw material - grapevine stems, a by-product of vine pruning. An efficient extraction-fractionation scheme is developed, based on shifting the phase equilibrium, by which more concentrated extracts of resveratrol are obtained. After a simple extraction, the initial extract is further separated into two fractions, containing either water or ethanol-soluble compounds. Using this approach, the resveratrol’s low water solubility helps isolate it from other water-soluble substances. The resulting product is almost ten times more concentrated in trans-resveratrol than the initial total extract. Additionally, a fraction containing water-soluble polyphenols is obtained, which could be used for water-based pharmaceutical and cosmetic preparations.

Optimization of High-Pressure Extraction Process of Antioxidant Compounds from Feteasca regala Leaves Using Response Surface Methodology

“…They concluded that a higher ration of solvent to solid ratio, 5:1 is better for the extraction. The difference between the ratio obtained in this research, 3:1, and the one Angelov et al [29], 5:1, can be explained based on the water content of the biomass used for the extortion. Leaves used in the current study have a much higher water content than grapevine stems.…”

“…Angelov et al [ 29 ] have studied the extraction of resveratrol from grapevine stems using a mixture of ethanol and water as the solvent [ 29 ]. They concluded that a higher ration of solvent to solid ratio, 5:1 is better for the extraction.…”

Viticultural wood waste as a source of polyphenols of interest: Opportunities and perspectives through conventional and emerging extraction methods

“…Pinelo et al (2005) demonstrated that an increase of this ratio (expressed in milliters of solvent per gram of solid, mL.g -1 ) from 1 to 5 mL.g -1 could dramatically improve the extraction of phenolic compounds from grape pomace. Angelov et al (2016) also approved this low limit of 5 mL.g -1 as it is accurate for good wetting of grapevine stems and to extract total phenolic content (TPC expressed in milligrams of gallic acid equivalents per gram of grape canes, mg(GAE).g -1 ). The authors also studied TPC extraction from globe artichoke and rosehip while increasing solventto-solid ratio (Angelov et al, 2014(Angelov et al, , 2015.…”

trans-Resveratrol and trans-ε-Viniferin in Grape Canes and Stocks Originating from Savoie Mont Blanc Vineyard Region: Pre-extraction Parameters for Improved Recovery

“…However, grapevine by-products are known to contain high-value molecules. 4,5 Among them, stilbenes are phenolic compounds having considerable importance for the grapevine since they defend the plant against aggressions from pathogenic microorganisms by acting as phytoalexins. 6 They are derived from the shikimicpolymalonic acid pathway and are biosynthesized by the combination of three malonyl CoA and one p-coumaroyl-CoA by stilbene synthase (StSy) to form trans-resveratrol, the first brick of stilbenoid compounds biosynthesis.…”