trans-Resveratrol and trans-ε-Viniferin in Grape Canes and Stocks Originating from Savoie Mont Blanc Vineyard Region: Pre-extraction Parameters for Improved Recovery

Abstract

Viticultural by-products are known to contain high-value compounds such as stilbenes. Wine sector in Savoie Mont Blanc region (France) generates high amount of waste including grape canes and grapevine stocks that remain unvalued. Herein, the potential of the most widespread grapevine varieties in Savoie Mont Blanc territory, Jacquère and Mondeuse, as sources of two stilbenes, trans-resveratrol (Rsv) and trans-ε-viniferin (Vf) was investigated for the first time. Using the reported extraction technique, the present work focused on several preextraction parameters influencing the extraction efficiency, including grapevine variety, age of 2 vines, time of material storage and particles size of the woody material. This work revealed important Rsv and Vf contents in Jacquère residues of 1681-6124 mg.kg-1 dw and 1620-6667 mg.kg-1 dw respectively, whereas for Mondeuse variety, the target molecules yields were 1724-4636 mg.kg-1 dw and 1713-5322 mg.kg-1 dw respectively. These results are promising when compared with high well-known stilbenes levels in Pinot Noir and Gewurztraminer varieties but also highlight a considerable variability depending on the applied pre-extraction parameters.

Bioactive compounds and total antioxidant capacity of cane residues from different grape varieties

“…Other studies have reported lower values with respect to our data for ϵ ‐viniferin, ranging from 2600 to 3300 μg g −1 DW in Sauvignon Blanc canes, 2200 μg g −1 DW in Cabernet Sauvignon, 1900 μg g −1 DW in Merlot, 1300 μg g −1 DW in Chardonnay, 2600 μg g −1 DW in Malbec and 3700 μg g −1 DW in Pinot Noir . Zwingelstein et al . reported values of ϵ ‐viniferin ranging from 1620 to 6667 μg g −1 DW in French grape varieties.…”

“…It was found at levels of between 6 (CH) and 250 μg g −1 (CS2), in accordance with the published data . However, the results obtained in the present study for trans ‐resveratrol were lower than in other reports, with values ranging from 122 to 7306 μg g −1 . Higher levels of trans ‐resveratrol than ϵ ‐viniferin may be a result of the influence of storage conditions (time, temperature, sample treatment) of canes .…”

“…The level of stilbenes and the composition of this by‐product depends on plant characteristics (genus, variety, age, etc. ), as well as growth environmental conditions and processing conditions after pruning . In turn, different stilbene profiles (qualitative and quantitative) can result as a result of chemical changes before (biosynthesis), during (mechanical wounding) or after (storage time and conditions) pruning of the grape plants .…”

Influence of pruning time and viral infection on stilbenoid levels in Pinot noir grape canes

“…It has already been demonstrated that, after cutting, grape canes if stored properly over some months, accumulate large quantities of stilbenoid . This increase depends on storage time, temperature, relative moisture during storage, grape variety, size of cane pieces during storage, and whether the grapes were cultivated using a conventional or organic mode . However, the combined effect of pruning and storage time on the stilbene accumulation rate has not been studied before.…”

“…In comparison, the antimicrobial properties of stilbenes have been investigated less. Due to its significance in both plant protection and human health, the content and distribution of resveratrol in grape and wine, and in the by‐products from grape and wine production, has attracted increasing attention in recent years …”