Under the conditions of an increasing anthropogenic load on marine ecosystems, bottom and pelagic communities can undergo significant changes, and even cardinal rearrangements. To assess coastal waters state and their further changes, information is needed both about individual components and ecosystems as a whole. Some biological characteristics of Far Eastern sea cucumber, gray sea urchin and scallop from different water areas have been investigated. At present, an urgent task is the artificial reproduction of aquatic organisms in order to preserve their number in natural populations by reducing the fishing pressure on them. An effective way of aquatic organisms replenishing natural accumulations and obtaining marketable products is technologies development and implementation for juveniles cultivation in factory conditions using feed preparations. The quality of probiotic supplements was assessed for compliance with regulatory documents requirements, as well as those declared by the manufacturer. This work results will serve as the basis for eco-friendly mariculture enterprises creation for the cultivation of aquatic organisms, taking into account the need for natural resources rational use and environmental protection.