Use of Whole Crop Sorghums as a Raw Material in Consolidated Bioprocessing Bioethanol Production Using<i>Flammulina velutipes</i>

Mizuno, Ryoji; Ichinose, Hitomi; Honda, Mariko; Takabatake, Koji; Sotome, Itaru; Takai, Tomoyuki; Maehara, Tomoko; Okadome, Hiroshi; Isobe, Seiichiro; Gau, Mitsuru; Kaneko, Satoshi

doi:10.1271/bbb.90099

Cited by 42 publications

(27 citation statements)

References 5 publications

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“…As shown in above, we found the edible mushroom F. velutipes Fv-1 strain to be an efficient ethanol producer, and, we demonstrated its preferable properties of ethanol fermentation from various sugars (Mizuno et al, 2009b), whole crop sorghums and rice straw (Mizuno et al, 2009a). However, the strain can only slightly convert pentoses, which account for approximately 20-30% of plant cell walls, into ethanol (Mizuno et al, 2009a).…”

Section: Development Of a Gene Transfer System For The Mycelia Of F supporting

confidence: 57%

“…Thus, the bmr mutation appears to be useful for CBP because it gives a high yield of glucose from biomass without acid or alkali pretreatment. However, the results indicate that the production of cellulases by F. velutipes is not sufficient for CBP, or that the saccharification enzymes are suppressed by carbon White, no added enzymes; black, 15 μl Celluclast 1.5 L and 10 μl Multifect xylanase added.6 Reproduced from Mizuno et al, 2009a) In this work, we demonstrated CBP ethanol fermentation of sorghum strains by F. velutipes Fv-1. The procedure is quite simple and cost effective, and can reduce energy consumption, because the raw material is simply ground and then mixed with mycelia.…”

Section: Properties Of Ethanol Production From Biomass By F Velutipesmentioning

confidence: 92%

“…However, the strain can only slightly convert pentoses, which account for approximately 20-30% of plant cell walls, into ethanol (Mizuno et al, 2009a). Therefore, genetic engineering of the pentose metabolism is necessary to make possible the ethanol fermentation from pentose.…”

Section: Development Of a Gene Transfer System For The Mycelia Of F mentioning

confidence: 99%

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Consolidated Bioprocessing Ethanol Production by Using a Mushroom

Kaneko¹,

Mizuno²,

Maehara³

et al. 2012

Bioethanol

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Section: Development Of a Gene Transfer System For The Mycelia Of F supporting

confidence: 57%

Section: Properties Of Ethanol Production From Biomass By F Velutipesmentioning

confidence: 92%

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Consolidated Bioprocessing Ethanol Production by Using a Mushroom

Kaneko¹,

Mizuno²,

Maehara³

et al. 2012

Bioethanol

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“…The final concentrations of ethanol produced from the two varieties of sorghum (mutated and wild type) were at about 200 and 180 g/l, respectively. They also managed to significantly increase ethanol yield by addition of cellulase and xylanase to the CBP process (Mizuno et al, 2009b).…”

Section: Flammulina Velutipesmentioning

confidence: 99%

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

2015

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HIGHLIGHTS Various CBP strategies have been discussed. Recently, lignocellulosic biomass as the most abundant renewable resource has been widely considered for bioalcohols production. However, the complex structure of lignocelluloses requires a multi-step process which is costly and time consuming. Although, several bioprocessing approaches have been developed for pretreatment, saccharification and fermentation, bioalcohols production from lignocelluloses is still limited because of the economic infeasibility of these technologies. This cost constraint could be overcome by designing and constructing robust cellulolytic and bioalcohols producing microbes and by using them in a consolidated bioprocessing (CBP) system. This paper comprehensively reviews potentials, recent advances and challenges faced in CBP systems for efficient bioalcohols (ethanol and butanol) production from lignocellulosic and starchy biomass. The CBP strategies include using native single strains with cellulytic and alcohol production activities, microbial co-cultures containing both cellulytic and ethanologenic microorganisms, and genetic engineering of cellulytic microorganisms to be alcohol-producing or alcohol producing microorganisms to be cellulytic. Moreover, high-throughput techniques, such as metagenomics, metatranscriptomics, next generation sequencing and synthetic biology developed to explore novel microorganisms and powerful enzymes with high activity, thermostability and pH stability are also discussed. Currently, the CBP technology is in its infant stage, and ideal microorganisms and/or conditions at industrial scale are yet to be introduced. So, it is essential to bring into attention all barriers faced and take advantage of all the experiences gained to achieve a high-yield and low-cost CBP process.

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“…F. velutipes could produce ethanol from sorghum. However, the author indicated that the production of cellulases from F. velutipes was not sufficient (Mizuno 2009a). Recently, the same group reported ethanol production from cultures containing high concentrations of sugarcane bagasse cellulose (prepared by treatment with 1 N NaOH) by F. velutipes.…”

Section: Cbp Fermentation Of High-concentration Uhkpmentioning

confidence: 99%

Direct Fungal Production of Ethanol from High-Solids Pulps by the Ethanol-fermenting White-rot Fungus Phlebia sp. MG-60

Kamei¹,

Hirota²,

Meguro³

2014

BioResources

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A white-rot fungus, Phlebia sp. MG-60, was applied to the fermentation of high-solid loadings of unbleached hardwood kraft pulp (UHKP) without the addition of commercial cellulase. From 4.7% UHKP, 19.6 g L -1 ethanol was produced, equivalent to 61.7% of the theoretical maximum. The highest ethanol concentration (25.9 g L -1 , or 46.7% of the theoretical maximum) was observed in the culture containing 9.1% UHKP. The highest filter paper activity (FPase) was observed in the culture containing 4.7% UHKP, while the production of FPase in the 16.5% UHKP culture was very low. Temporarily removing the silicone plug from Erlenmeyer flasks, which relieved the pressure and allowed a small amount of aeration, improved the yield of ethanol produced from the 9.1% UHKP, which reached as high as 37.3 g L -1 . These results indicated that production of cellulase and ensuing saccharification and fermentation by Phlebia sp. MG-60 is affected by water content and benefits from a small amount of aeration.

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Use of Whole Crop Sorghums as a Raw Material in Consolidated Bioprocessing Bioethanol Production UsingFlammulina velutipes

Cited by 42 publications

References 5 publications

Consolidated Bioprocessing Ethanol Production by Using a Mushroom

Consolidated Bioprocessing Ethanol Production by Using a Mushroom

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

Direct Fungal Production of Ethanol from High-Solids Pulps by the Ethanol-fermenting White-rot Fungus Phlebia sp. MG-60

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