Use of Tris(hydroxymethyl)aminomethane as a Test Substance in Reaction Calorimetry.

Determination of optimal glucose concentration for microcalorimetric metabolic evaluation of equine spermatozoa

“…Standard calibration plots were described as follows: A=1.028xq+0.0266, in which A is the integrated area under the output envelope, and q is the amount of heat released. These results were also reported by Mares-Guia et al, 1990 andIrving andWadsö , 1964, in which calibration was also performed by neutralization of Tris by HCl, based on heat of (-) 47234 J/mole of neutralized H + .…”

“…To determine the reproducibility of the calorimetric experiment, four calibrations using different concentrations of HCl (0.5 mM; 1.0 mM; 2.0 mM; 4.0 mM) were performed (Irving and Wadsö, 1964). The heat released, between 2 and 25 mJ, corresponded to the neutralization energy between 0.2 µmol and 2.5 µ mol of H + , respectively.…”

Design of a Precision Calorimetric System for Measuring Heats of Solution

“…The heats of reaction were found to be independent of concentration, and the average of six values was found to be -7116 ± 15 cal/mole, which is slightly higher than the value of -7104 cal/mole reported by Irving and Wadso [ 11].…”

Application of Solution Calorimetry in Pharmaceutical and Biopharmaceutical Research

“…A number of chemical test reactions for solutions calorimeters have been proposed and discussed, including the dissolution of Tris in 0.1 M HCl [5,11], the dissolution of KCl or NaCl in water [12][13][14] and the dissolution of propan-1-ol in water [15]. The dissolution of sucrose in water can also be used [16][17], although this is not currently recognised as a test reaction.…”