Use of Triplex PCR for Rapid Detection of PVL and Differentiation of MRSA from Methicillin Resistant Coagulase Negative Staphylococci

Abimanyu, Nagarajan

doi:10.7860/jcdr/2013/4523.2731

Cited by 14 publications

(11 citation statements)

References 21 publications

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“…According to the PCR results, 28 out of 126 (22.22%) S. aureus isolates were pvl carriers. In 2013, Abimanyu et al in India reported that 10 out of 25 (40%) MRSA isolates were positive for pvl genes [28] which was higher than the results of our study. In another study in Iran, Ohadian Moghadam et al showed that 14.3% of the 56 isolated S. aureus collected from Isfahan were PVL-positive [29].…”

Section: Discussioncontrasting

confidence: 91%

Genotyping and characterization of prophage patterns in clinical isolates of Staphylococcus aureus

et al. 2019

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ObjectiveStaphylococcus aureus is considered an important pathogen with a variety of virulence factors in communities and hospitals all around the world. Prophage typing is a practical technique for categorizing this bacterium. In this study, we focused on the detection of prophage patterns in methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) strains based on their virulence factors, antimicrobial resistance patterns, and molecular typing by rep-PCR.ResultsOut of 126 S. aureus isolates, 45 (35.7%) were identified as MRSA. In total, 17 different prophage types were detected and 112 strains out of 126 strains contained at least one prophage. There was a statistically significant relationship between hld, hlg, eta and SGA, SGA, and SGFb, respectively. The results of the rep-PCR analysis revealed 14 different patterns among the MRSA and MSSA isolates. In conclusion, the presence of different prophage-encoded virulence factors and antibiotic-resistant genes among MRSA strains enables them to produce a broad range of diseases. Thus, diverse MRSA strains which have these prophages can be considered as a potential threat to the patient’s health in either the hospital or the community.

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Section: Discussioncontrasting

confidence: 91%

Genotyping and characterization of prophage patterns in clinical isolates of Staphylococcus aureus

et al. 2019

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“…in India in 2013 using Triplex PCR for the detection of PVL MRSA in 100 clinical isolates of Staphylococcus spp. [ 17 ] reported that 10 out of 25 (40%) MRSA isolates were positive for PVL gene, which was higher than the results of this study. S. aureus gained resistance to antibiotics and spread within the hospital environment, so the prevalence rate of resistant S. aureus is relatively high.…”

Section: Discussioncontrasting

confidence: 83%

Molecular Epidemiology Survey of Staphylococcus aureus Panton–Valentine Leukocidin-positive Isolated from Sanandaj, Iran

et al. 2017

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Background:Staphylococcus aureus strains that are Panton–Valentine leukocidin (PVL) positive cause severe skin and soft tissue infections as well as necrotizing pneumonia. The presence of PVL gene is a marker for methicillin-resistant S. aureus; therefore, survey on prevalence and phylogenetic distribution of PVL is of great importance for public health. The aim of this research was molecular epidemiology survey of S. aureus PVL positive, isolated from two tertiary hospitals of Sanandaj.Materials and Methods:A total of 264 staphylococci isolates were collected from clinical specimens, hospital personnel and hospital environment of two tertiary hospitals of Sanandaj, in 2012 (Toohid and Besat). Bacterial cultures and biochemical tests were performed for S. aureus detection. Then, polymerase chain reaction (PCR) and repetitive sequence-based PCR (rep-PCR) were used for the determination of prevalence and molecular epidemiology of S. aureus PVL, respectively. Data were analyzed using the Fisher's exact test (P < 0.05).Results:From 264 staphylococci isolates, 88 (33.33%) were detected as S. aureus. Furthermore, 20 out of 88 (22.72%) strains of S. aureus were PVL positive according to PCR results. Rep-PCR showed six main clusters of S. aureus samples. PVL had similar clonality between different samples. No significant relationship was observed between PVL positive S. aureus and rep-PCR patterns (P = 0.98).Conclusion:These results showed that a clone of S. aureus PVL positive has spread between the community and hospital settings. Therefore, appropriate measures are required to prevent the spread of staphylococci and other bacteria in hospitals.

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“…DNA extraction was done by the modified method of Abimanyu et al, [14]. Few bacterial colonies were suspended in 300µl of DNase free water (Qiagen) and kept in dry bath (Labnet) for 10 min, kept in freezer (-20ºC) overnight.…”

Section: (D) Dna Extraction -Boiling Lysis Methodsmentioning

confidence: 99%

“…Methicillin resistance was screened by using cefoxitin (30µg) disc diffusion method and Multiplex PCR (M-PCR) was performed for the simultaneous detection and differentiation of MRSA from MRCoNS [14]. S. aureus ATCC 43300 and S. epidermidis RP62A were used as positive control.…”

Section: (E) Screening Of Methicillin Resistance By Phenotypic and Gementioning

confidence: 99%

Analysis of Antibiotic Resistance Genes and its Associated SCC mec Types among Nasal Carriage of Methicillin Resistant Coagulase Negative Staphylococci from Community Settings, Chennai, Southern India

Saravanan

Perumal

Mahalingam

et al. 2015

JCDR

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Use of Triplex PCR for Rapid Detection of PVL and Differentiation of MRSA from Methicillin Resistant Coagulase Negative Staphylococci

Cited by 14 publications

References 21 publications

Genotyping and characterization of prophage patterns in clinical isolates of Staphylococcus aureus

Genotyping and characterization of prophage patterns in clinical isolates of Staphylococcus aureus

Molecular Epidemiology Survey of Staphylococcus aureus Panton–Valentine Leukocidin-positive Isolated from Sanandaj, Iran

Analysis of Antibiotic Resistance Genes and its Associated SCC mec Types among Nasal Carriage of Methicillin Resistant Coagulase Negative Staphylococci from Community Settings, Chennai, Southern India

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