Use of Transverse Microradiography to Quantify Mineral Loss by Erosion in Bovine Enamel

Amaechi, Bennett T.; Higham, Susan; Edgar, W.M.

doi:10.1159/000016471

Cited by 56 publications

(50 citation statements)

References 21 publications

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“…In a previous study by De Josselin et al 5) , TMRW software (Inspektor Research Systems BV, Amsterdam, the Netherlands) 17) was used to measure tooth mineralization. In that study 5) , the Vol of sound enamel (Vs) was 90.2 0.9, which was in good agreement with the values of 90.7 1.3 by CCD camera and 90.6 1.0 by FS procedure in this study.…”

Section: Discussionmentioning

confidence: 99%

Development of New Software as a Convenient Analysis Method for Dental Microradiography

et al. 2007

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To the end of developing a convenient research tool to calculate the mineralization status of teeth in detail, a new program was developed using Visual Basic for Applications combined with Microsoft Excel 2004. To demonstrate the usefulness of this program, it was used to analyze tooth enamel mineralization after acid exposure. Transverse microradiography images (TMR) of specimens were digitalized with a charge-coupled device camera with a microscope (CCD camera) and a digital film scanner (FS). Subsequently, the mineral content profile of each specimen after de-and remineralization studies were calculated using the Angmar's formula. The newly developed program was applied to calculating the mineral loss (ΔZ), lesion depth (Ld), surface zone depth (SZd), and lesion body depth (LBd) of tooth specimens. In addition, the outer surface zone (OSZ), inner lesion body (ILB), and sandwich area (SA) between OSZ and ILB -which together constituted ΔZ -were calculated by the newly developed program. Data obtained with the newly developed program were in good agreement for both CCD camera and FS, indicating that the program was reliable for tooth enamel mineralization research studies.

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Section: Discussionmentioning

confidence: 99%

Development of New Software as a Convenient Analysis Method for Dental Microradiography

et al. 2007

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“…Note that in contrast to TMR, as is usually applied in caries research, the erosion is performed on the cut sur-face of an already prepared tooth slice of 100-200 tJm thickness rather than on a specimen's surface that is cut perpendicularly for TMR after an experimental procedure. Hall et al [86] [65] found a strong correlation between mineral loss determined by either TMR or profilometry even for discrimination of early ero-sive lesions caused by erosion times of less than 1 h. Another approach to use TMR for erosive mineral loss determination also depends on the use of reference areas not subjected to an erosive challenge [87] [88] [66]. The erosive challenge is executed on a specimens' surface.…”

Section: Chemical Analysis Of Minerals Dissolved In the Erosive Agentmentioning

confidence: 99%

“…In comparison, watertreated samples showed indentation depth of 150-350 nm [115] [87]. The nanoindenter could be coupled to the vertical transducer used in combination with AFM, where the cantilever and the laser-optical system is replaced by the transducer-tip system allowing for determination of tip displacements with 0.2 nm resolution [107] [116] [82].…”

Section: Atomic Force Microscopymentioning

confidence: 99%

Methods for Assessment of Dental Erosion

Attin

Wegehaupt²

2014

Monographs in Oral Science

125

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Various assessment techniques have been applied to evaluate the loss of dental hard tissue and the surface-softened zone in enamel induced by erosive challenges. In this chapter, the most frequently adopted techniques for analyzing the erosively altered dental hard tissues are reviewed, such as profilometry, measuring microscope techniques, microradiography, scanning electron microscopy, atom force microscopy, nano-and microhardness tests and iodide permeability test. Moreover, methods for chemical analysis of minerals dissolved from dental hard tissue are discussed. It becomes evident that the complex nature of erosive mineral loss and dissolution might not be comprehended by a single technique, but needs application of different approaches for full understanding. Chapter 10Lussi A (ed): Dental Erosion. Monogr Oral Sci. Basel, Karger, 2006, vol 20, pp 152-172 Methods for Assessment of Dental Erosion Thomas Attin, Florian Just WegehauptCenter for Dental Medicine, Clinic for Preventive Dentistry, Periodontology and Cariology, University of Zurich, Zürich, Switzerland AbstractVarious assessment techniques have been applied to evaluate the loss of dental hard tissue and the surface-softened zone in enamel induced by erosive challenges. In this chapter, the most frequently adopted techniques for analyzing the erosively altered dental hard tissues are reviewed, such as profilometry, microradiography, scanning electron microscopy, atom force microscopy, nano-and microhardness tests and iodide permeability test. Moreover, methods for chemical analysis of minerals dissolved from dental hard tissue are discussed. It becomes evident that the complex nature of erosive mineral loss and dissolution might not be comprehended by a single technique, but needs application of different approaches for full understanding. Copyright © 2006 S. Karger AG, Basel Acid attack leads to an irreversible loss of the outermost enamel and dentin layers and to partial demineralization (softening) of the tooth surface. In enamel, the thickness of the softened layer is estimated to be 2-5 tJm [1,2] [1, 2]. The softened eroded tooth surface is highly susceptible to abrasive wear, and mechanical impacts such as toothbrushing can easily remove the superficially demineralized dental hard tissue [3][4][5] [3][4][5]. For simulating intra-oral erosion as closely as possible, it is desirable to assess the erosive effects on native tooth surfaces. Most of the methods described below need polished surfaces for precise assessment of the erosively induced defects or for creating reference surfaces, which means that the natural, often fluoridated surface of the tooth has to be removed. However, it should be considered that in the case of intra-oral erosion the outermost surface layers are also continuously removed by the acid attack, so that a 'polished' surface is Attin 2 cre-ated. When monitoring of erosive surface alterations within a period of time is performed, it could become necessary to fix a specimen in the measuring device i...

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“…Current techniques of characterising the surface of an eroded lesion include surface micro hardness (SMH), 40 profilometry, scanning electron microscopy (SEM) 41 micro radiography, 42 digital image analysis, 43 chemical analysis, iodide permeability and atomic force microscopy (AFM). Quantitative measurement for mineral loss is preferable to qualitative results gained from surface profilometry and scanning electron microscopy.…”

Section: Measurement Of the Eroded Surfacementioning

confidence: 99%

Modern methods for assessing the cariogenic and erosive potential of foods

Curzon¹,

Hefferren²

2001

Br Dent J

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RESEARCH nutrition and plaque pH, but also determined that enamel demineralisation should be further developed as a model. Dental erosion was not perceived as a problem, at that time, and was not mentioned in the deliberations. The animal model has continued to be used. Researchers, and other authorities, wishing to determine the cariogenic potential of foods, drinks and some oral medicines, have used the plaque pH models. The enamel demineralisation model, often called the intra-oral cariogenicity test (ICT), has been further developed since the San Antonio conference, so that it is now widely used for cariogenicity testing. Variants of the ICT, also known as the in situ model, are now being developed to test foods and drinks for erosion, but need evaluation.Accordingly on 8 and 9 November 1999, a group of dental scientists and clinicians met in London (UK) to review the San Antonio recommendations, reassess them and draw up revised guidelines. The aim was to determine which methods are currently suitable as research tools but also for regulatory assessments.The conference was directed by Professor John Hefferren, previously Chairman of the San Antonio meeting, and who lead the discussions of all delegates. In each of the four workshop sessions, under the guidance of a chairman, a presenter gave a prepared report to update the information on a particular method or group of methodologies. Each presentation was then commented on by a reactor and then by open discussion of all delegates. On the second day individual workshops were held involving small groups of delegates and working documents prepared presenting draft new guidelines.In a final plenary session all of the working documents were reconsidered and refined. Subsequently, the chairman of each working group was asked to prepare a draft document that was then circulated and commented upon by all delegates. After final editing the consensus opinions are presented in this document. Organisation of the textThe following text is organised to consider the four main groups of models for cariogenicity and erosion evaluation. The first is the animal model, usually based on the rat using an automatic feeding machine. The second group concerns the various approaches to assess plaque pH responses to the use of foods. The third model is the enamel demineralisation method and the fourth is that concerning assessment of the erosive potential of foods. Readers should consider this document in association with the San Antonio publication 1 which gives the original guidelines for methodologies in great detail. Herein only suggested changes or comments on the original have been made. Animal models:Workshop group: M. E. J. Curzon, J. J. Hefferren, P. M. Marsh and P. Mtolo The group agreed that animal testing (usually with the laboratory rat) remains an important model to assess cariostatic and cario-

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Use of Transverse Microradiography to Quantify Mineral Loss by Erosion in Bovine Enamel

Cited by 56 publications

References 21 publications

Development of New Software as a Convenient Analysis Method for Dental Microradiography

Development of New Software as a Convenient Analysis Method for Dental Microradiography

Methods for Assessment of Dental Erosion

Modern methods for assessing the cariogenic and erosive potential of foods

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