Use of traditional plants in management of Halitosis in a Moroccan poulation

Akkaoui, Sanae; Ennibi, Oum Keltoum

doi:10.5455/jice.20170522103525

Cited by 17 publications

(11 citation statements)

References 46 publications

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“…However, there are few reports on the effects of Moroccan EOs on periodontal pathogens. The EO of O. vulgare was selected for this study on the basis of its traditional use for the treatment of oral diseases [13].…”

Section: Discussionmentioning

confidence: 99%

“…Morocco has, by its geographical diversity, great natural resources for cultivation of medicinal and aromatic plants. Surveys performed in Morocco among the population in different regions have shown a frequent therapeutic use of this natural heritage in traditional medicine [12,13].…”

Section: Introductionmentioning

confidence: 99%

“…However, the use of medicinal plants and essential oils is limited in dentistry, and their antimicrobial activities on oral bacteria are not widely studied. We have previously reported that, in Moroccan population, O.vulgare is used as a mouthwash in traditional medicine [13].…”

Section: Introductionmentioning

confidence: 99%

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Chemical Composition, Antimicrobial activity, In Vitro Cytotoxicity and Leukotoxin Neutralization of Essential Oil from Origanum vulgare against Aggregatibacter actinomycetemcomitans

et al. 2020

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In this study, the essential oil of Origanum vulgare was evaluated for putative antibacterial activity against six clinical strains and five reference strains of Aggregatibacter actinomycetemcomitans, in comparison with some antimicrobials. The chemical composition of the essential oil was analyzed, using chromatography (CG) and gas chromatography–mass spectrometry coupled (CG–MS). The major compounds in the oil were Carvacrol (32.36%), α-terpineol (16.70%), p-cymene (16.24%), and Thymol (12.05%). The antimicrobial activity was determined by an agar well diffusion test. A broth microdilution method was used to study the minimal inhibitory concentration (MIC). The minimal bactericidal concentration (MBC) was also determined. The cytotoxicity of the essential oil (IC50) was <125 µg/mL for THP-1 cells, which was high in comparison with different MIC values for the A. actinomycetemcomitans strains. O. vulgare essential oil did not interfere with the neutralizing capacity of Psidium guajava against the A. actinomycetemcomitans leukotoxin. In addition, it was shown that the O. vulgare EO had an antibacterial effect against A. actinomycetemcomitans on a similar level as some tested antimicrobials. In view of these findings, we suggest that O.vulgare EO may be used as an adjuvant for prevention and treatment of periodontal diseases associated to A. actinomycetemcomitans. In addition, it can be used together with the previously tested leukotoxin neutralizing Psidium guajava.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chemical Composition, Antimicrobial activity, In Vitro Cytotoxicity and Leukotoxin Neutralization of Essential Oil from Origanum vulgare against Aggregatibacter actinomycetemcomitans

et al. 2020

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“…Plants and other natural products have been extensively used in the management of oral infections and improvement of oral health in many populations (Akkaoui & Ennibi, ; Chinsembu, ). Among several tested herbal plants, we found that extract of leaves or twigs from Psidium guajava efficiently neutralizes the activity of A. actinomycetemcomitans leukotoxin (Kwamin, Gref, Haubek, & Johansson, ).…”

Section: Introductionmentioning

confidence: 99%

High salivary levels of JP2 genotype of Aggregatibacter actinomycetemcomitans is associated with clinical attachment loss in Moroccan adolescents

Ennibi

Claesson

Akkaoui

et al. 2019

Clinical & Exp Dental Res

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It has previously been shown that the presence of Aggregatibacter actinomycetemcomitans in subgingival plaque is significantly associated with increased risk for clinical attachment loss. The highly leukotoxic JP2 genotype of this bacterium is frequently detected in adolescents with aggressive forms of periodontitis. The aims of the study were to quantify the levels of JP2 and non‐JP2 genotypes of A. actinomycetemcomitans in saliva of Moroccan adolescents with the JP2 genotype earlier detected in the subgingival plaque. The salivary concentrations of inflammatory proteins were quantified and linked to the clinical parameters and microbial findings. Finally, a mouth rinse with leukotoxin‐neutralizing effect was administrated and its effect on the levels the biomarkers and A. actinomycetemcomitans examined. The study population consisted of 22 adolescents that previously were found to be positive for the JP2 genotype in subgingival plaque. Periodontal registration and sampling of stimulated saliva was performed at baseline. A mouth rinse (active/placebo) was administrated, and saliva sampling repeated after 2 and 4 weeks rinse. The salivary levels of JP2 and non‐JP2 were analyzed by quantitative PCR and inflammatory proteins by ELISA. Both the JP2 and the non‐JP2 genotype were detected in all individuals with significantly higher levels of the non‐JP2. Enhanced levels of the JP2 genotype of A. actinomycetemcomitans was significantly correlated to the presence of attachment loss (≥3 mm). Salivary concentrations of inflammatory biomarkers did not correlate to periodontal condition or levels of A. actinomycetemcomitans . The use of active or placebo leukotoxin‐neutralizing mouth rinse did not significantly interfered with the levels of these biomarkers. Saliva is an excellent source for detection of A. actinomycetemcomitans on individual basis, and high levels of the JP2 genotype were significantly associated with the presence of clinical attachment loss.

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“…Several recent studies related to the use of S. aromaticum extract and its essential oil content for the treatment of periodontal diseases including halitosis have been reported. A cross-sectional survey by Akkaoui and Ennibi (2017) reported that there were 23 medicinal plants including clove commonly used by Moroccan herbalists for the treatment of halitosis. A study by Kaur and Chandrul (2017) showed that the main component of clove oil was eugenol, with β-caryophyllene and eugenyl acetate; but the contents of essential oils in cloves were found to be various depending on their anatomic and geographical origins.…”

Section: Introductionmentioning

confidence: 99%

Syzygium aromaticum essential oil prevents halitosis caused by oral bacteria Streptococcus sanguinis

Yanti¹,

Juniardi²,

Lay³

2019

Food Res.

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Halitosis is caused by oral bacteria including Streptococcus sanguinis in mouth producing volatile sulphur compounds (VSCs), such as hydrogen sulfide, ethyl mercaptan, and methyl mercaptan which have pungent odor. Bacteria producing sulphur compounds produce oral biofilms as the accumulation of caries promotion. Caries is caused by the acid produced by oral bacteria that lead to tooth demineralization in low pH condition. Clove bud (Syzygium aromaticum), known as endogenous spice in Indonesia, has been traditionally used for centuries for treatment of periondal diseases. In this study, we extracted essential oil from Syzygium aromaticum (SAEO), identifed for its major essential oils by pyrolysis gas chromatograpy mass spectrometry (py-GC/MS), determined its antihalitosis efficacy on preventing and eradicating S. sanguinis oral biofilms, inhibiting VSCs and acid production in vitro. Chromatogram profile showed that SAEO contained major eugenol (22.10%) and aceteugenol (13.31%). For antibiofilm effect toward oral bacteria S. sanguinis, SAEO at 40 and 60 μg/mL effectively prevented biofilm formation up to 60% and removed the existed biofilms up to 50%. SAEO at 60 μg/mL also demonstrated a significant inhibition on VSCs production (up to 58%) and acid produced by S. sanguinis by increasing the terminal pH from 5.66 to 6.30. These data suggest that SAEO could be applied for a promising candidate for developing oral care functional products for management of halitosis.

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Use of traditional plants in management of Halitosis in a Moroccan poulation

Cited by 17 publications

References 46 publications

Chemical Composition, Antimicrobial activity, In Vitro Cytotoxicity and Leukotoxin Neutralization of Essential Oil from Origanum vulgare against Aggregatibacter actinomycetemcomitans

Chemical Composition, Antimicrobial activity, In Vitro Cytotoxicity and Leukotoxin Neutralization of Essential Oil from Origanum vulgare against Aggregatibacter actinomycetemcomitans

High salivary levels of JP2 genotype of Aggregatibacter actinomycetemcomitans is associated with clinical attachment loss in Moroccan adolescents

Syzygium aromaticum essential oil prevents halitosis caused by oral bacteria Streptococcus sanguinis

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