Use of the synbiotic VSL#3 and yacon-based concentrate attenuates intestinal damage and reduces the abundance of Candidatus Saccharimonas in a colitis-associated carcinogenesis model

Chen

et al. 2021

DDDT

Total flavone of Abelmoschus manihot (TFA), the effective constituents extracted from Flos Abelmoschus Manihot, has been reported to inhibit inflammation. However, the effect of TFA on ulcerative colitis (UC) progression in patients with depression is unknown. The purpose of our research was to explore the anti-UC effects of TFA in the context of depression in mice with UC by regulating the gut microbiota to drive the intestinal barrier. Methods: In this study, chronic stress (CS) and dextran sodium sulfate (DSS) were used to induce depression and UC, respectively, in C57BL/6J mice. Fecal microbiota transplantation (FMT) was used to evaluate how treating mice modeling UC and depression with TFA effected their gut microbiota. Results: Our results showed that TFA effectively improved UC aggravated by CS. In addition, TFA treatment improved the depression-like phenotype, the disturbed gut microbiota, and the intestinal barrier function in CS mice. It is worth noting that FMT from the CS mice to the receptor group further aggravated the damage of the intestinal barrier and the disturbance of the gut microbiota in the recipient DSS mice, thus further aggravating UC, however, treatment of the intervention of TFA in the CS fecal microbiota transplant with TFA also played its therapeutic outcome. Conclusion:Taken together, our results show that CS disrupts the gut microbiota, triggers intestinal barrier injury and aggravates DSS colitis, while TFA is a promising drug for the treatment of UC in patients with depression.

Section: Discussionmentioning

confidence: 99%

Total Flavone of Abelmoschus manihot Ameliorates Stress-Induced Microbial Alterations Drive Intestinal Barrier Injury in DSS Colitis

Chen

et al. 2021

DDDT

Polish Journal of Microbiology

“…The consumption of probiotics has been reported to modulate the composition and structure of the gut microbiome and treat multiple diseases, but some functional activities are strain-specific (Biagioli et al 2019;Hsu et al 2019). There are also various methods for detecting gut microbiota, among which 16S rRNA amplicon sequencing is a faster and cheaper way to study the gut microbiome (Goldfeder et al 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Wang et al (2018) found that L. plan tarum ZDY2013 remits ulcerative colitis by modifying of intestinal microbiota to regulate both oxidative stress and inflammatory mediators. Some functional activities are strain-specific (Biagioli et al 2019). Qiu et al (2018) injected mice with potential probiotic strains, including L. plantarum ZDY04 (PLA04) and L. plantarum ZDY01 (PLA01).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Lactobacillus plantarum BW2013 on The Gut Microbiota in Mice Analyzed by 16S rRNA Amplicon Sequencing

Tong

Niu

et al. 2021

Lactobacillus plantarum BW2013 was isolated from the fermented Chinese cabbage. This study aimed to test the effect of this strain on the gut microbiota in BALB/c mice by 16S rRNA amplicon sequencing. The mice were randomly allocated to the control group and three treatment groups of L. plantarum BW2013 (a low-dose group of 108 CFU/ml, a medium-dose group of 109 CFU/ml, and a high-dose group of 1010 CFU/ml). The weight of mice was recorded once a week, and the fecal samples were collected for 16S rRNA amplicon sequencing after 28 days of continuous treatment. Compared with the control group, the body weight gain in the treatment groups was not significant. The 16S rRNA amplicon sequencing analysis showed that both the Chao1 and ACE indexes increased slightly in the medium-dose group compared to the control group, but the difference was not significant. Based on PCoA results, there was no significant difference in β diversity between the treatment groups. Compared to the control group, the abundance of Bacteroidetes increased in the low-dose group. The abundance of Firmicutes increased in the medium-dose group. At the genus level, the abundance of Alloprevotella increased in the low-dose group compared to the control group. The increased abundance of Ruminococcaceae and decreased abundance of Candidatus_Saccharimonas was observed in the medium-dose group. Additionally, the abundance of Bacteroides increased, and Alistipes and Candidatus_Saccharimonas decreased in the high-dose group. These results indicated that L. plantarum BW2013 could ameliorate gut microbiota composition, but its effects vary with the dose.

“…For example, the effects of co-administration of the probiotic VSL#3 and the prebiotic yacon ( Smallanthus sonchifolius , a tuberous root rich in phenolic compounds and with a high soluble fiber content) were tested in a model of colitis-associated carcinogenesis. The synbiotic demonstrated numerous potential benefits: it supported the integrity of the intestinal barrier, increased the concentrations of SCFAs, as well as enzymes involved in the endogenous antioxidant defense system, and led to alterations in the general composition of the intestinal microbiota [ 111 ]. Furthermore, in a model of carcinogenesis induced by 1,2-dimethylhydrazine (DMH), treatment with VSL#3 and yacon reduced the size and number of pre-neoplastic lesions.…”

Section: Synbioticsmentioning

confidence: 99%

“…Furthermore, in a model of carcinogenesis induced by 1,2-dimethylhydrazine (DMH), treatment with VSL#3 and yacon reduced the size and number of pre-neoplastic lesions. In particular, the synbiotic was found to increase the secretion of IL-2 and IL-4; the former has effects on the regulation of immune cells and has been inversely correlated with tumor size, while the latter occurs concomitantly with the expression of TLR4, resulting in the improvement of the innate immune response and antitumor defense [ 111 ]. In a similar study, Lin et al [ 97 ] demonstrated the protective effect of a combination of germinated brown rice (GBR) and L. acidophilus / Bifidobacterium animalis subsp.…”

Section: Synbioticsmentioning

confidence: 99%

Role of Gut Microbiota and Probiotics in Colorectal Cancer: Onset and Progression

et al. 2021

The gut microbiota plays an important role in maintaining homeostasis in the human body, and the disruption of these communities can lead to compromised host health and the onset of disease. Current research on probiotics is quite promising and, in particular, these microorganisms have demonstrated their potential for use as adjuvants for the treatment of colorectal cancer. This review addresses the possible applications of probiotics, postbiotics, synbiotics, and next-generation probiotics in colorectal cancer research.