Use of the Secreted Proteome of Trametes versicolor for Controlling the Cereal Pathogen Fusarium langsethiae

Abstract

Fusarium langsethiae is amongst the most recently discovered pathogens of small grains cereals. F. langsethiae is the main producer, in Europe, of T2 and HT-toxins in small grain cereals, albeit often asymptomatic; this makes its control challenging. The European Union (EU) is pushing hard on the use of biocontrol agents to minimize the use of fungicides and pesticides, which are detrimental to the environment and responsible for serious pollution of the soil and superficial water. In line with EU directives (e.g., 128/2009), here we report the use of protein fractions, purified from the culture filtrate of the basidiomycete Trametes versicolor, for controlling F. langsethiae. T. versicolor, a so-called medicinal mushroom which is applied as a co-adjuvant in oncology and other pathologies as a producer of biological response modifiers. In this study, the exo-proteome of T. versicolor proved highly efficient in inhibiting the growth of F. langsethiae and the biosynthesis of the T2 toxin. Results are promising for its future use as a sustainable product to control F. langsethiae infection in cereals under field conditions.

Oligosaccharides Derived from Tramesan: Their Structure and Activity on Mycotoxin Inhibition in Aspergillus flavus and Aspergillus carbonarius

“…In addition, nowadays the EC policy is driving research to investigate more environmentally friendly “green” approaches. This has increased the research on the development of biocontrol strategies as well as the use of natural plant extracts to control and mitigate the presence of mycotoxins in food and feed [ 20 , 21 , 22 , 23 , 24 ]. One of the promising tools for mycotoxin (particularly aflatoxins) control, with lower environmental impact, are the metabolites of higher mushrooms.…”

The Effect of Mushroom Culture Filtrates on the Inhibition of Mycotoxins Produced by Aspergillus flavus and Aspergillus carbonarius

“…In particular, the discovery of the very active part of the polysaccharide would be especially relevant, from an economic point of view, as its synthesis could be more cost effective. Moreover, fungal and plant extracts could represent a promising tool in mycotoxin control, compared to chemicals [ 39 , 40 , 41 , 42 , 43 ], due to their lower toxicity when released into the environment.…”

“…After incubation, the mycelia were separated from the culture filtrates by subsequent filtrations through 0.45 μm filters (Sartorius, Goettingen, Germany), to eliminate all the mycelia. Mycelia-purified culture filtrates were concentrated in rota-vapor (Rotavapor ® R-300, Buchi, Essen City, Germany), and up to a concentration of 100 mL as described by Parroni and collaborators [ 42 ]. Concentrated culture filtrates were lyophilized and utilized for subsequent analyses as reported in [ 24 ].…”

Effects of Rhizosphere Microbial Composition on the Growth of Cajanus Cajan in Coal Gangue Reclaimed Soil

“…Cruz analyzed the effects of soil conditions (soil texture, pH, and soil moisture) on Fusarium graminearum when planting (Jimenez et al, 2020). Parroni et al (2019) reported the use of Trametes versicolor for Controlling the cereal pathogen Fusarium langsethiae. Wei (2019) screened for Acrocalymma from soil around the coal mine and found that Acrocalymma formed a symbiotic relationship with alfalfa and sand Holly and promoted the uptake of nitrogen and phosphorus by plants.…”