Use of the polymerase chain reaction in differentiating rinderpest field virus and vaccine virus in the same animals

Barrett, Thomas; Amarel-Doel, C; Rp, Kitching; Gusev, A. A.

doi:10.20506/rst.12.3.734

Cited by 33 publications

(14 citation statements)

References 12 publications

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“…CDV and PDV formed another cluster and DMV was separated in a different cluster with high bootstrap confidence. The tree also confirms similar grouping of morbilliviruses as observed earlier [5,8,11].…”

Section: Phylogenetic Analysis Of the F And H Genesupporting

confidence: 86%

“…A total of 16 cysteine residues identified in this protein of PPRV-S were similar to PPRV-N of which 12 are conserved across morbilliviruses, indicating the conservatory nature of this amino acid in maintaining the tertiary structure . of this protein [5]. The paramyxovirus fusion protein normally synthesized as precursor F0, which is cleaved in to two subunits, F1 and F2 linked by a disulfide bond [4].…”

Section: Resultsmentioning

confidence: 99%

“…The other members of this genus include rinderpest virus (RPV), measles virus (MV), canine distemper virus (CDV), phocine distemper virus (PDV), dolphin morbillivirus (DMV) and porpoise morbillivirus (PMV) [5,6]. The genome of morbilliviruses is a single stranded-RNA,~16 kb long with negative polarity [7].…”

Section: Introductionmentioning

confidence: 99%

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Sequence Analysis of the Haemagglutinin and Fusion Protein Genes of Peste-des-petits Ruminants Vaccine Virus of Indian Origin

et al. 2006

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The amino acid composition of the two surface proteins of peste-des-petits ruminants vaccine virus belonging to lineage four from India were deduced from the nucleotide sequence. The fusion (F) protein gene of PPRV Sungri/96 is 2405 nucleotides long and in relation to the length, it is 80 nucleotides longer than that of PPRV Nigeria/75/1 which are found to be present at the 5'UTR of this virus. The complete F gene alignment with other morbillivirus reveals a homology of 89% with PPRV/Nigeria/75/1 and 48-51% with other morbilliviruses. The F protein of PPRV Sungri/96 exhibited characteristics similarity to those of other morbillivirus F proteins. The overall amino acid similarity with its counterpart PPRV Nigeria/75/1 was 96%; with other morbilliviruses it is 65-74%. The PPRV Sungri/96 haemagglutinin (H) protein gene is 1954 nucleotides long and showed a sequence homology of 90.7% with PPRV/Nigeria/75/1 and with other morbilliviruses it ranged from 33% to 45%. At amino acids level, PPRV Sungri/96 showed a homology of 92.3% with PPRV/Nigeria/75/1 and 34-49% with other morbilliviruses. The phylogenetic tree constructed for F and H gene reveals four separate groups which is very similar to that found in other genes. To the best of our knowledge this is the first report describing the F and H genes of an Indian isolate.

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Section: Phylogenetic Analysis Of the F And H Genesupporting

confidence: 86%

Section: Resultsmentioning

confidence: 99%

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Sequence Analysis of the Haemagglutinin and Fusion Protein Genes of Peste-des-petits Ruminants Vaccine Virus of Indian Origin

et al. 2006

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“…This is the first study in which vaccine virus and wild virus have been distinguished in vaccinated patients (24). A similar distinction between vaccine and field viruses using the PCR has been demonstrated for rinderpest virus (4). However, this approach can only be applied to viruses isolated from clinical cases and is not practical for routine use, especially in the veterinary field.…”

Section: Differentiation Between Natural Infection and Vaccinationmentioning

confidence: 85%

Progress in the development of a heat-stable recombinant rinderpest using an attenuated vaccinia virus vector

Yamanouchi¹,

Barrett²

1994

Rev. Sci. Tech. OIE

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Rinderpest is a fatal infectious disease of cattle and buffalo, and is prevalent in many parts of the developing world. Eradication campaigns are at present under way in Africa, the Middle East and South Asia. As these regions have very hot climates, it is difficult and expensive to establish reliable cold chains to deliver heat-sensitive vaccines and, for this reason, many previous vaccination/eradication campaigns have been ineffective. To overcome the problem of vaccine heat-lability, a recombinant rinderpest vaccine (RRV) has been developed by inserting the haemagglutinin gene of rinderpest virus into the attenuated smallpox vaccine (vaccinia virus) and using this as a heat-stable vaccine vector system to deliver the foreign antigens. This rinderpest vaccine, as with the smallpox vaccine, could be used for the global eradication campaign, without the need to establish a cold chain. Both the efficacy and the safety of the RRV have been confirmed in experiments in cattle. In addition to heat stability, the RRV has several other advantages over the current rinderpest tissue-culture vaccine. DNA viruses have greater genetic stability than RNA viruses (this may be easily checked by restriction enzyme analysis), and the use of RRV enables vaccinated animals to be distinguished from naturally-infected animals, as the vaccine generates a more restricted antigenic response to rinderpest virus. The current situation in relation to the efficacy and safety of the RRV is discussed.

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“…In future this PCR-based sequencing analysis will facilitate the study of epidemiology and will enable accurate backtracing of the source of any new virus epizootic. The technique also proved to be capable of differentiating rinderpest field virus and vaccine virus in the same animal (BARRETT et al, 1993b). Using primers based on well-conserved regions in the respective F and P genes, it is possible to distinguish PRV from PPRV field isolates (FORSETH and BARRET~, 1996).…”

Section: Molecular Phylogenymentioning

confidence: 99%

Rinderpest and Other Animal Morbillivirus Infections: Comparative Aspects and Recent Developments

Haas

Barrett

1996

Journal of Veterinary Medicine, Series B

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Summary The genus morbillivirus presently comprises measles virus of man, rinderpest virus (RPV), peste des petits ruminants virus (PPRV), and canine distemper virus (CDV). ‘Emerging’ morbilliviruses, such as phocid distemper virus (PDV) of seals, dolphin (DMV) and porpoise morbillivirus (PMV) have probably been present for a long period of time and outbreaks are possibly related to introduction into a highly susceptible population and/or be the result of interspecies transmission. In this review some comparative aspects of morbillivirus infections, particularly with respect to rinderpest and canine distemper viruses, are presented. Topics include pathogenesis, epidemiology, molecular phylogeny, diagnosis and prophylaxis. Recent developments in molecular biology have created tools which have enabled us to achieve a better understanding of morbillivirus infections at the nucleic acid level (‘molecular epidemiology’) while recombinant DNA technology has allowed new bivalent recombinant vaccines with improved heat stability to be produced.

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Use of the polymerase chain reaction in differentiating rinderpest field virus and vaccine virus in the same animals

Cited by 33 publications

References 12 publications

Sequence Analysis of the Haemagglutinin and Fusion Protein Genes of Peste-des-petits Ruminants Vaccine Virus of Indian Origin

Sequence Analysis of the Haemagglutinin and Fusion Protein Genes of Peste-des-petits Ruminants Vaccine Virus of Indian Origin

Progress in the development of a heat-stable recombinant rinderpest using an attenuated vaccinia virus vector

Rinderpest and Other Animal Morbillivirus Infections: Comparative Aspects and Recent Developments

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