Use of the polymerase chain reaction to study arthritis due to <i>neisseria gonorrhoeae</i>

Muralidhar, Bethi; Rumore, Peter; Steinman, Charles R.

doi:10.1002/art.1780370515

Cited by 44 publications

(11 citation statements)

References 14 publications

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“…23 Likewise, specific nucleic acids were shown to persist in gonococcal arthritis a few days after the start of therapy. 24 These converging data corroborate current observations and highlight the interest of molecular testing in patients receiving antibiotherapy, in whom the rate of false-negative culture can reach 50%. 5 The current protocol involved collecting and sending separate samples for molecular and bacteriological analyses.…”

Section: Discussionsupporting

confidence: 77%

Amplification-Based DNA Analysis in the Diagnosis of Prosthetic Joint Infection

Vandercam

Jeumont

Cornu

et al. 2008

The Journal of Molecular Diagnostics

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Microbiological cultures are moderately sensitive for diagnosing prosthetic joint infection (PJI). This study was conducted to determine whether amplificationbased DNA methods applied on intraoperative samples could enhance PJI diagnosis compared with culture alone in routine surgical practice. Revision arthroplasty was performed for suspected PJI (n ‫؍‬ 41) and osteoarthrosis control (n ‫؍‬ 28) patients , and a diagnosis of PJI was confirmed in 34 patients. Amplification by polymerase chain reaction was performed on both 16S ribosomal DNA universal target genes and femA Staphylococcus-specific target genes. Species identification was achieved through amplicon sequencing. Amplification of the femA gene led to subsequent testing for methicillin resistance by amplification of the mecA gene. Microbiological and molecular assays identified a causative organism in 22 of 34 patients (64.7%) and in 31 of 34 patients (91.2%), respectively. In 18 of the 22 culture-positive patients, molecular and microbiological results were concordant for bacterial genus , species , and/or methicillin resistance. Bacterial agents were identified only by molecular methods in nine PJI patients , including seven who were receiving antibiotics at the time of surgery and one with recent but not concomitant antibiotherapy. DNA-based methods were found to effectively complement microbiological methods , without interfering with existing procedures for sample collection , for the identification of causative pathogens from intraoperative PJI samples , especially in patients with recent or concomitant antibiotherapy. (J Mol

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Section: Discussionsupporting

confidence: 77%

Amplification-Based DNA Analysis in the Diagnosis of Prosthetic Joint Infection

Vandercam

Jeumont

Cornu

et al. 2008

The Journal of Molecular Diagnostics

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“…That article and others 18,26,40,42 have demonstrated that PCR can detect small numbers of pathogenic organisms in synovial fluid specimens. Infectious organisms have also been extracted from saliva, 41 cerebrospinal fluid, 6 blood, 8,43,45,47Y49 and bone aspirates 27 with methods that neutralize inhibitors to the PCR.…”

Section: Discussionmentioning

confidence: 93%

The Use of Polymerase Chain Reaction for the Detection and Speciation of Bacterial Bone and Joint Infection in Children

Song

Boatright

Drassler

et al. 2009

Journal of Pediatric Orthopaedics

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We evaluated 36 consecutive patients presenting with signs and symptoms of bacterial bone and joint infection and 10 control patients using bacterial cultures of blood and the presumed site of infection compared with polymerase chain reaction (PCR) techniques using a universal primer and restriction endonuclease digestion. Of the 28 patients with definitive clinical and/or laboratory evidence of bacterial infection, 16 patients had positive bacterial cultures and 12 were PCR-positive. Twenty of 28 patients were either PCR- or culture-positive. Nine of the 16 subjects who had culture-positive samples also had PCR-positive samples (8 positive for the same organism and 1 with 2 organisms identified by culture, but only a single organism by PCR. Six culture positive patients were PCR-negative. Of the 12 patients who were culture-negative, 4 had bacterial genomic material present indicating infection. We conclude that current PCR methods are not superior to standard bacterial culture methods when applied to children with presumed bone or joint infections, but that PCR may complement existing microbiologic cultures for detection of bone and joint infections in children.

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“…Another report describes the persistence of nucleic acids of Neisseria gonorrhoeae during the first few days of antibiotic therapy (10). Of interest, the microorganism can be cultured in SF samples in only 25-50% of the patients with clinical gonococcal arthritis (10). This was also the case in 1 of the SF samples prior to the initiation of antibiotic therapy in patient 1.…”

Section: Discussionmentioning

confidence: 98%

“…This patient had persisting evidence of Staphylococcus aureus DNA in the SF from this knee joint up to 10 weeks after therapy was started, whereas SF cultures did not yield any growth (9). Another report describes the persistence of nucleic acids of Neisseria gonorrhoeae during the first few days of antibiotic therapy (10). Of interest, the microorganism can be cultured in SF samples in only 25-50% of the patients with clinical gonococcal arthritis (10).…”

Section: Discussionmentioning

confidence: 99%