2012
DOI: 10.1590/s0100-879x2012007500011
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Use of the polymerase chain reaction to detect Mycobacterium leprae in urine

Abstract: Leprosy is an infectious disease caused by Mycobacterium leprae. The polymerase chain reaction (PCR) has been applied to detect M. leprae in different clinical samples and urine seems to be attractive for this purpose. PCR was used to improve the sensitivity for diagnosing leprosy by amplifying a 151-bp PCR fragment of the M. leprae pra gene (PCR-Pra) in urine samples. Seventy-three leprosy patients (39 males and 34 females, 14 to 78 years old) were selected for leprosy diagnosis at a reference laboratory in M… Show more

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Cited by 31 publications
(19 citation statements)
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“…It is clear that effective control of leprosy requires, together with multidrug therapy, new diagnostic tools that can detect M. leprae infection at an early stage. The PCR technique has been evaluated for DNA detection of M. leprae in different systems [ 18 20 ].…”
Section: Discussionmentioning
confidence: 99%
“…It is clear that effective control of leprosy requires, together with multidrug therapy, new diagnostic tools that can detect M. leprae infection at an early stage. The PCR technique has been evaluated for DNA detection of M. leprae in different systems [ 18 20 ].…”
Section: Discussionmentioning
confidence: 99%
“…Another method targets a gene that encodes an antigenic 36 kDa protein known as proline-rich antigen (pra gen) by cPCR [28][29][30][31][32][33], and can detect up to a single bacteria in the sample [28]. The pra gen has also been amplified by multiplex PCR (mPCR) [34]. Plikaytis and coworkers, developed a nPCR that amplifies a heat shock protein 65 kDa called groEL, which can detect 3 fg of M. leprae-DNA (single bacteria) [35].…”
Section: Leprae Detection In Clinical Samplesmentioning
confidence: 99%
“…The use of urine as a diagnosis sample is currently being evaluated [34,96]. The possibility of amplifying the M. leprae DNA in a simple non-invasive sample can be useful and maybe improve the diagnosis mainly in the tuberculoid form or in inconclusive cases where the slit skin smear is usually negative (PNL, IL and PB patients) [34].…”
Section: Urinementioning
confidence: 99%
“…Polymerase chain reaction (PCR) has been used to detect M. leprae in lymph (Phetsuksiri, et al, 2006), blood (Almeida, 2004), nasal swab (Almeida, 2004), tissue fragment (Phetsuksiri, et al, 2006;Martinez, 2006), and urine (Caleffi et al, 2012(Caleffi et al, , 2010Parkash et al, 2004) clinical samples. Different PCR primers have been used to amplify the DNA of M. leprae from clinical samples (Kang, Kim, Lee, 2003).…”
Section: Introductionmentioning
confidence: 99%