Use of the insertion element IS6110 for DNA fingerprinting of Mycobacterium tuberculosis isolates presenting various profiles of drug susceptibility

Thierry, Dominique

doi:10.1016/0928-8244(93)90005-o

Cited by 6 publications

(6 citation statements)

References 24 publications

(37 reference statements)

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“…The low rate of primary drug resistance observed in the present study showed that, despite the substantial difficulties in Tanzania, as in all other developing countries, the national TB control program in Tanzania has proved to be quite efficient. In accordance with previous findings (6,26), there was no correlation between DNA fingerprint patterns and drug resistance. However, phenotypic characteristics which classify M. tuberculosis strains as being of the Asian subgroup correlated with a low IS6110 copy number per isolate.…”

Section: Discussionsupporting

confidence: 91%

DNA fingerprinting and phenotyping of Mycobacterium tuberculosis isolates from human immunodeficiency virus (HIV)-seropositive and HIV-seronegative patients in Tanzania

et al. 1995

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With the purpose of determining whether the risk of infection with a particular clone of Mycobacterium tuberculosis is influenced by the human immunodeficiency virus (HIV) status of the host, we analyzed and compared 68 mycobacterial isolates obtained from HIV-seropositive patients with tuberculosis (TB) in Dar esSalaam, Tanzania, with 66 mycobacterial isolates obtained from HIV-seronegative patients with TB in the same geographical region by using both DNA fingerprinting and classical phenotyping methods. One hundred one different IS6110 fingerprinting patterns were observed in the 134 isolates. The level of diversity of the DNA fingerprints observed in the HIV-seropositive group was comparable to the level of the diversity observed in the HIV-seronegative group. Resistance to a single anti-TB drug was found in 8.8% of the tested isolates, and 3.2% of the isolates were resistant to more than one anti-TB drug. The drug susceptibility profiles were not significantly difference between the two groups of isolates compared in the present study. Phenotypic characteristics which classify M. tuberculosis strains as belonging to the Asian subgroup correlated with a low IS6110 copy number per isolate. However, the occurrence of Asian subgroup strains was not associated with the HIV status of the patients. The results of the study suggested an equal risk of infection with a defined M. tuberculosis clone for HIV-seropositive and HIV-seronegative individuals.

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Section: Discussionsupporting

confidence: 91%

DNA fingerprinting and phenotyping of Mycobacterium tuberculosis isolates from human immunodeficiency virus (HIV)-seropositive and HIV-seronegative patients in Tanzania

et al. 1995

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“…These findings are in agreement with previous observations. 15 The related strains had identical profiles, with the exception of the strains obtained from the two members of family B and those of family D. The slight difference in the IS6110-containing banding patterns of B1 and B2 strains, and D1 and D2 strains strongly suggests that an IS transposition has occurred. 16 Moreover, it is worth noting that B1 and B2 strains showed different profiles of susceptibility to antituberculous drugs (B1 strain was rifampin-susceptible, while B2 was rifampin-resistant).…”

Section: Discussionmentioning

confidence: 88%

Molecular characterization of Mycobacterium tuberculosis isolates presenting various drug susceptibility profiles from Greece using three DNA typing methods

Vrioni¹

2004

Journal of Infection

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“…Restriction fragment length polymorphism (RFLP) analysis based on the insertion sequence IS6110 results in a unique genotype since both the number of copies of this genetic element and its positions in the genome are variable (25,26). Precise IS6110 insertion site mapping provides additional information on the fitness of the strain (1) given that IS6110 insertion can modify the expression of the gene involved.…”

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confidence: 99%

Molecular Characteristics of Strains of the Cameroon Family, the Major Group of Mycobacterium tuberculosis in a Country with a High Prevalence of Tuberculosis

Niobe-Eyangoh

Kuaban

Sorlin³

et al. 2004

J Clin Microbiol

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A preliminary investigation of the genetic biodiversity of Mycobacterium tuberculosis complex strains in Cameroon, a country with a high prevalence of tuberculosis, described a group of closely related M. tuberculosis strains (the Cameroon family) currently responsible for more than 40% of smear-positive pulmonary tuberculosis cases. Here, we used various molecular methods to study the genetic characteristics of this family of strains. Cameroon family M. tuberculosis strains (i) are part of the major genetic group 2 and lack the TbD1 region like other families of epidemic strains, (ii) lack spacers 23, 24, and 25 in their direct repeat ( Molecular epidemiology methods revolutionized the fields of research, prevention, and control of tuberculosis (TB), allowing the differentiation between strains, assessment of the overall diversity of Mycobacterium tuberculosis complex strains including differences by region and population, and measurement of the prevalence of endemic strains (28). However, few molecular epidemiological studies have been conducted in countries with a high incidence of TB. The available data suggest that families of closely related strains are common in these areas (12). The "Beijing family" is one of the most well-known families, highly prevalent in East Asia and widespread around the world (11).Molecular analysis based on several variable genomic regions is required for a good definition of strains belonging to different families. Restriction fragment length polymorphism (RFLP) analysis based on the insertion sequence IS6110 results in a unique genotype since both the number of copies of this genetic element and its positions in the genome are variable (25,26). Precise IS6110 insertion site mapping provides additional information on the fitness of the strain (1) given that IS6110 insertion can modify the expression of the gene involved. Another genetic element useful for characterizing tubercle bacilli is the direct repeat (DR) locus (13), a polymorphic insertion preferential locus (ipl) for IS6110. DR polymorphism can be analyzed by spoligotyping, a method involving PCR-reverse hybridization (14). The DR locus is likely to evolve more slowly than IS6110, making spoligotyping less adequate than IS6110-RFLP for discriminating strains but more convenient for investigating the biogeographic distribution of families of M. tuberculosis complex strains (32). Variable-number tandem repeats, named mycobacterial interspersed repetitive units (MIRU-VNTR), are another type of variable element in the M. tuberculosis complex genome showing extensive polymorphism (17) with a discrimination power close to that of IS6110-RFLP (6). Because of their stability, they can be used for a clear definition of families of tubercle bacilli as well.Strains of the M. tuberculosis complex exhibit very little genome sequence diversity except in repeat sequences. Consequently, insignificant and rare genome alterations inherited and maintained through long-term evolution can be of phylogenetic value. On the basis of polymorphi...

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Use of the insertion element IS6110 for DNA fingerprinting of Mycobacterium tuberculosis isolates presenting various profiles of drug susceptibility

Cited by 6 publications

References 24 publications

DNA fingerprinting and phenotyping of Mycobacterium tuberculosis isolates from human immunodeficiency virus (HIV)-seropositive and HIV-seronegative patients in Tanzania

DNA fingerprinting and phenotyping of Mycobacterium tuberculosis isolates from human immunodeficiency virus (HIV)-seropositive and HIV-seronegative patients in Tanzania

Molecular characterization of Mycobacterium tuberculosis isolates presenting various drug susceptibility profiles from Greece using three DNA typing methods

Molecular Characteristics of Strains of the Cameroon Family, the Major Group of Mycobacterium tuberculosis in a Country with a High Prevalence of Tuberculosis

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