Molecular characterization of Mycobacterium tuberculosis isolates presenting various drug susceptibility profiles from Greece using three DNA typing methods

Vrioni, Georgia

doi:10.1016/j.jinf.2003.09.007

Cited by 7 publications

(3 citation statements)

References 27 publications

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“…10 They recommend DRE-PCR as a second-line test that would be very useful especially in developing countries and/or areas with high prevalence of TB. Based on our results, which are in agreement with the recent data of Vrioni et al, 25 we would suggest to apply DRE-PCR as a screening method to test a large number of M. tuberculosis isolates, followed by IS6110 RFLP analysis of clusters to retain only epidemiologically linked cases.…”

Section: Discussionsupporting

confidence: 91%

Molecular characterization of Mycobacterium tuberculosis isolates from Łódź, Poland: Analysis by IS6110 restriction fragment length polymorphism and double-repetitive-element PCR

Dela

Sajduda

Pawłowska

et al. 2006

Journal of Infection

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Section: Discussionsupporting

confidence: 91%

Molecular characterization of Mycobacterium tuberculosis isolates from Łódź, Poland: Analysis by IS6110 restriction fragment length polymorphism and double-repetitive-element PCR

Dela

Sajduda

Pawłowska

et al. 2006

Journal of Infection

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“…The completion of the regime will take up to 9 months [ 65 ]. On the other hand, multidrug-resistant tuberculosis associated infections are treated by streptomycin, capreomycin and quinolones [ 99 ]. Nevertheless, a study by Garhyan et al showed that the Mycobacterium tuberculosis can reside in dormancy in bone marrow-mesenchymal stem cells (BM-MSCs) and persist in the intracellular milieu even after 3 months of extensive pyrazinamide and isoniazid treatment [ 131 ].…”

Section: Challenges Of Conventional Antimicrobial Treatments For Omentioning

confidence: 99%

Recent Updates on Treatment of Ocular Microbial Infections by Stem Cell Therapy: A Review

Teh

Mok

Rashid

et al. 2018

IJMS

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Ocular microbial infection has emerged as a major public health crisis during the past two decades. A variety of causative agents can cause ocular microbial infections; which are characterized by persistent and destructive inflammation of the ocular tissue; progressive visual disturbance; and may result in loss of visual function in patients if early and effective treatments are not received. The conventional therapeutic approaches to treat vision impairment and blindness resulting from microbial infections involve antimicrobial therapy to eliminate the offending pathogens or in severe cases; by surgical methods and retinal prosthesis replacing of the infected area. In cases where there is concurrent inflammation, once infection is controlled, anti-inflammatory agents are indicated to reduce ocular damage from inflammation which ensues. Despite advances in medical research; progress in the control of ocular microbial infections remains slow. The varying level of ocular tissue recovery in individuals and the incomplete visual functional restoration indicate the chief limitations of current strategies. The development of a more extensive therapy is needed to help in healing to regain vision in patients. Stem cells are multipotent stromal cells that can give rise to a vast variety of cell types following proper differentiation protocol. Stem cell therapy shows promise in reducing inflammation and repairing tissue damage on the eye caused by microbial infections by its ability to modulate immune response and promote tissue regeneration. This article reviews a selected list of common infectious agents affecting the eye; which include fungi; viruses; parasites and bacteria with the aim of discussing the current antimicrobial treatments and the associated therapeutic challenges. We also provide recent updates of the advances in stem cells studies on sepsis therapy as a suggestion of optimum treatment regime for ocular microbial infections.

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“…Genetic molecular typing by means of RAPD-PCR has been successfully used in clinical-epidemiological studies of different species in the genus Mycobacterium (MatsiotaBernard et al 2000;Zhang et al 2002;Tiwari et al 2003;Esteban et al 2006) including within the MTC isolates of de M. bovis (Milian-Suazo et al 2000) and M. tuberculosis (Linton et al 1994;Harn et al 1997;Tazi et al 2004;Vrioni et al 2004;Korzekwa et al 2006;Ramasoota et al 2006;Singh et al 2006). It has been stated that the main limitations of the use of RAPDs is reproducibility (Fritsch and Rieseberg 1996) because the results may be influenced by the concentrations of magnesium chloride and DNA and by the primer to DNA ratio, among other factors.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity among Mycobacterium tuberculosis isolates from Mexican patients

et al. 2008

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Forty-six isolates of the Mycobacterium tuberculosis complex were typified by PCR of the IS6110 region and by Mycobacterium bovis specific primers JB21/JB22. Isolate MVG01 was typified as M. bovis, being the first record of a case of human tuberculosis caused by this species in Mexico. RAPD-PCR was used to describe the genetic diversity of the remaining 45 M. tuberculosis complex isolates. The corrected genotypic diversity value calculated for the analyzed population was 0.96, the estimated mean gene diversity was 0.235, and the corrected Shannon-Weiner index was 2.15. All allele-loci combinations generated showed significant linkage disequilibria. The distribution of genetic variation was analyzed both by the unweighted pair group method with arithmetic averages clustering and by principal coordinates analysis. Unweighted pair group method with arithmetic averages clustering resulted in a tree with four main clusters and one unclustered strain (MVG20), the principal coordinates analysis strain distribution pattern being consistent with this grouping. The obtained results suggest that the studied isolates belong to a clonal population having significant genetic diversity. Our genetic diversity results are comparable with those reported for other populations of M. tuberculosis, although only three RAPD primers were used.

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Molecular characterization of Mycobacterium tuberculosis isolates presenting various drug susceptibility profiles from Greece using three DNA typing methods

Cited by 7 publications

References 27 publications

Molecular characterization of Mycobacterium tuberculosis isolates from Łódź, Poland: Analysis by IS6110 restriction fragment length polymorphism and double-repetitive-element PCR

Molecular characterization of Mycobacterium tuberculosis isolates from Łódź, Poland: Analysis by IS6110 restriction fragment length polymorphism and double-repetitive-element PCR

Recent Updates on Treatment of Ocular Microbial Infections by Stem Cell Therapy: A Review

Genetic diversity among Mycobacterium tuberculosis isolates from Mexican patients

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