Use of the diagnostic bacteriology laboratory: a practical review for the clinician

Steinbach, William J.; Shetty, Avinash K.

doi:10.1136/pmj.77.905.148

Cited by 22 publications

(9 citation statements)

References 16 publications

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“…Morphologically and biochemically different isolates were selected for further study. Morphological features studied included colony texture, margins, colour, elevation, growth, form, shape of bacterial cells (rod, cocci or coccobacillus) and Gram’s staining reaction (Steinbach and Shetty 2001 ). Biochemical characterisation was done using Bacterial identification kit (HiMedia KB003) as per user manual instructions (Results available as supplementary data).…”

Section: Methodsmentioning

confidence: 99%

Diversity of culturable bacterial endophytes of saffron in Kashmir, India

2015

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Saffron (Crocus sativus) is a medicinally important plant. The Kashmir valley (J&K, India) emblematizes one of the major and quality saffron producing areas in the world. Nonetheless, the area has been experiencing a declining trend in the production of saffron during the last decade. Poor disease management is one of the major reasons for declining saffron production in the area. Endophytes are known to offer control against many diseases of host plant. During the present study, culturable bacterial endophytes were isolated from saffron plant, identified and assessed for plant growth promoting activities. Molecular and phylogenetic analysis grouped the fifty-four bacterial isolates into eleven different taxa, viz. Bacillus licheniformis, B. subtilis, B. cereus, B. humi, B. pumilus, Paenibacillus elgii, B. safensis, Brevibacillus sp., Pseudomonas putida, Staphylococcus hominis and Enterobacter cloacae. The results were also supported with the identification based on BIOLOG system. B. licheniformis was the dominant endophyte in both leaves and corms of saffron. 81 % isolates showed lipase activity, 57 % cellulase, 48 % protease, 38 % amylase, 33 % chitinase and 29 % showed pectinase activity. 24 % of the isolates were phosphate solublizers, 86 % showed siderophore production and 80 % phytohormone production potential. The present repository of well characterized bacterial endophytes of saffron, have plant growth promoting potential which can be explored further for their respective roles in the biology of the saffron plant.Electronic supplementary materialThe online version of this article (doi:10.1186/s40064-015-1435-3) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Diversity of culturable bacterial endophytes of saffron in Kashmir, India

2015

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“…Bacterial endophytes were grouped on the basis of their phenotypic characteristics, for example, colony colour and morphology, Gram reaction staining (Steinbach and Shetty ) and their antagonistic activity against selected fungi. All bacterial strains were further grouped into their taxa based on ITS cloning and sequencing.…”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of indigenous endophytic bacteria associated with leaves of switchgrass (Panicum virgatum L.) cultivars

et al. 2013

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Aims: To isolate and characterize indigenous bacterial endophytes from cultivars of switchgrass and study their antimicrobial and growth promoting potential. Methods and Results: The diversity, molecular and biochemical characterizations of indigenous and culturable bacterial endophytes residing in leaves of switchgrass have not been studied previously. This study describes the characterization of 31 bacterial endophytes from three switchgrass cutlivars: Cave-in Rock, Blue Jacket and Tecumseh. Molecular and phylogenetic analysis based on the 16S rRNA sequence grouped the endophytes into eight different taxa that shared high homology of 98-99% with other known sequences. Bacterial endophytes were identified as Microbacterium testaceum, Curtobacterium flaccumfaciens, Bacillus subtilis and Bacillus pumilus, Pseudomonas fluorescens, Sphingomonas parapaucimobilis, Serratia sp. and Pantoea ananatis. Some endophytes were detected in switchgrass seeds and in plants that originated from seeds collected a year earlier, confirming vertical transmission to the next generation of the host. Selected endophytes produced cellulases and were capable of solubilizing inorganic phosphorus. Analysis of cell-free culture filtrate of selected strains using direct infusion orbitrap mass spectrometry confirmed the presence of several well-characterized lipopeptide toxins and phytohormones. Re-inoculation of the roots of switchgrass seedlings with endophytes singly or combined confirmed their migration to the upper aerial parts of the plant. Conclusions: Our findings suggest that switchgrass leaves harbour a diversity of bacterial endophytes, some of which could potentially be applied as growth promoting bacteria. Significance and Impact of the Study: This is the first report on the characterization of indigenous bacterial endophytes and their potential use as biofertilizers.

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“…Bacteria: One hundred and thirty-four (134) bacterial strains were grown on LB at room temperature for 16-18 h with agitation (175 RPM) to achieve concentrations between 10 8 -10 10 CFU.mL -1 . Cells were collected for Gram reaction staining (Steinbach and Shetty 2001), and DNA extraction using the Presto TM Mini gDNA Bacteria Kit (FroggaBio, ON, Canada) according to the manufacturer's instructions. DNA quality was confirmed on 1 % agarose gel prior to subsequent reactions.…”

Section: Molecular Identification and Characterization Of Endophytesmentioning

confidence: 99%

Endophytes of industrial hemp (Cannabis sativaL.) cultivars: identification of culturable bacteria and fungi in leaves, petioles, and seeds

et al. 2018

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Plant endophytes are a group of microorganisms that reside asymptomatically within the healthy living tissue. The diversity and molecular and biochemical characterization of industrial hemp-associated endophytes have not been previously studied. This study explored the abundance and diversity of culturable endophytes residing in petioles, leaves, and seeds of three industrial hemp cultivars, and examined their biochemical attributes and antifungal potential. A total of 134 bacterial and 53 fungal strains were isolated from cultivars Anka, CRS-1, and Yvonne. The number of bacterial isolates was similarly distributed among the cultivars, with the majority recovered from petiole tissue. Most fungal strains originated from leaf tissue of cultivar Anka. Molecular and phylogenetic analyses grouped the endophytes into 18 bacterial and 13 fungal taxa, respectively. The most abundant bacterial genera were Pseudomonas, Pantoea, and Bacillus, and the fungal genera were Aureobasidium, Alternaria, and Cochliobolus. The presence of siderophores, cellulase production, and phosphorus solubilization were the main biochemical traits. In proof-of-concept experiments, re-inoculation of tomato roots with some endophytes confirmed their migration to aerial tissues of the plant. Taken together, this study demonstrates that industrial hemp harbours a diversity of microbial endophytes, some of which could be used in growth promotion and (or) in biological control designed experiments.

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Use of the diagnostic bacteriology laboratory: a practical review for the clinician

Cited by 22 publications

References 16 publications

Diversity of culturable bacterial endophytes of saffron in Kashmir, India

Diversity of culturable bacterial endophytes of saffron in Kashmir, India

Isolation and characterization of indigenous endophytic bacteria associated with leaves of switchgrass (Panicum virgatum L.) cultivars

Endophytes of industrial hemp (Cannabis sativaL.) cultivars: identification of culturable bacteria and fungi in leaves, petioles, and seeds

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