Use of TALEs and TALEN Technology for Genetic Improvement of Plants

Khan, Zulqurnain; Khan, Sultan Habibullah; Mubarik, Muhammad Salman; Sadia, Bushra; Ahmad, Aftab

doi:10.1007/s11105-016-0997-8

Cited by 36 publications

(24 citation statements)

References 155 publications

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“…Nevertheless, TALENs have been used for the targeted mutagenesis of another legume, namely soybean (Haun et al, 2014;Demorest et al, 2016;Du et al, 2016;Curtin et al, 2018). The use of TALENs for the mutagenesis of higher plants was recently reviewed by Malzahn et al (2017) and Khan et al (2017).…”

Section: Talensmentioning

confidence: 99%

Biotechnological Perspectives of Omics and Genetic Engineering Methods in Alfalfa

et al. 2020

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For several decades, researchers are working to develop improved major crops with better adaptability and tolerance to environmental stresses. Forage legumes have been widely spread in the world due to their great ecological and economic values. Abiotic and biotic stresses are main factors limiting legume production, however, alfalfa (Medicago sativa L.) shows relatively high level of tolerance to drought and salt stress. Efforts focused on alfalfa improvements have led to the release of cultivars with new traits of agronomic importance such as high yield, better stress tolerance or forage quality. Alfalfa has very high nutritional value due to its efficient symbiotic association with nitrogenfixing bacteria, while deep root system can help to prevent soil water loss in dry lands. The use of modern biotechnology tools is challenging in alfalfa since full genome, unlike to its close relative barrel medic (Medicago truncatula Gaertn.), was not released yet. Identification, isolation, and improvement of genes involved in abiotic or biotic stress response significantly contributed to the progress of our understanding how crop plants cope with these environmental challenges. In this review, we provide an overview of the progress that has been made in high-throughput sequencing, characterization of genes for abiotic or biotic stress tolerance, gene editing, as well as proteomic and metabolomics techniques bearing biotechnological potential for alfalfa improvement.

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Section: Talensmentioning

confidence: 99%

Biotechnological Perspectives of Omics and Genetic Engineering Methods in Alfalfa

et al. 2020

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“…In the last decade, artificial zinc-finger proteins (AZPs) have been used against begomoviruses (beet severe curly top virus (BSCTV) and tomato yellow leaf curl Targeted Genome Editing for Cotton Improvement http://dx.doi.org/10.5772/intechopen.73600 virus (TYLCV), respectively) [43,44]. This strategy can be used for suppression of begomoviruses infecting cotton plants [45]. Moreover, ZFNs and AZPs can be useful for gene insertion, deletion, replacement, and functional genomics studies in cotton.…”

Section: Zinc-finger Nucleasesmentioning

confidence: 99%

“…Golden gate assembly of Cermak et al has advantage of being fast, simple, and cost-effective [1]. Many free online softwares are available to design TALEs and TALENs [45]. The assembly of TALENs has also been offered on commercial basis by different companies, and many kits are available to construct TALENs against target sequence [45].…”

Section: Tales and Talensmentioning

confidence: 99%

“…Many free online softwares are available to design TALEs and TALENs [45]. The assembly of TALENs has also been offered on commercial basis by different companies, and many kits are available to construct TALENs against target sequence [45]. These TALE domains can be linked with a designed effector domain (nuclease like Fok1, repressor like KRAB, or activator like VP64) to create a chimeric protein capable of targeted genome manipulation.…”

Section: Tales and Talensmentioning

confidence: 99%

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Targeted Genome Editing for Cotton Improvement

Khan¹,

Khan²,

Mubarik³

et al. 2018

Past, Present and Future Trends in Cotton Breeding

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Conventional tools induce mutations randomly throughout the cotton genome-making breeding difficult and challenging. During the last decade, progress has been made to edit the gene of interest in a very precise manner. Targeted genome engineering with engineered nucleases (ENs) specifically zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat (CRISPR) RNA-guided nucleases (e.g., Cas9) has been described as a "game-changing technology" for diverse fields as human genetics and plant biotechnology. In eukaryotic systems, ENs create double-strand breaks (DSBs) at the targeted DNA sequence which are repaired by nonhomologous end joining (NHEJ) or homologydirected recombination (HDR) mechanisms. ENs have been used successfully for targeted mutagenesis, gene knockout, and multisite genome editing (GenEd) in model plants and crop plants such as cotton, rice, and wheat. Recently, cotton genome has also been edited for targeted mutagenesis through CRISPR/Cas for improved lateral root formation. In addition, an efficient and fast method has been developed to evaluate guide RNAs transiently in cotton. The targeted disruption of undesirable genes or metabolic pathway can be achieved to increase quality of cotton. Undesirable metabolites like gossypol in cottonseed can be targeted efficiently using ENs for seed-specific low-gossypol cotton. Moreover, ENs are also helpful in gene stacking for herbicide resistance, insect resistance, and abiotic stress tolerance.

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“…Like clustered regularly interspersed short palindromic/ Cas systems, TALEs are programmable DNA binding proteins with potential for applications in synthetic biology. By replacing the naturally occurring activation domain (AD) with other executer domains, TALEs were converted into artificial sequence specific nucleases, transcription repressors, methylases, and recombinases among others (de Lange et al, 2014;Khan et al, 2017). TALEs were also converted into inducible transcription regulators.…”

mentioning

confidence: 99%

Split-TALE: A TALE-Based Two-Component System for Synthetic Biology Applications in Planta

et al. 2019

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Transcription activator-like effectors (TALEs) are bacterial Type-III effector proteins from phytopathogenic Xanthomonas species that act as transcription factors in plants. The modular DNA-binding domain of TALEs can be reprogrammed to target nearly any DNA sequence. Here, we designed and optimized a two-component AND-gate system for synthetic circuits in plants based on TALEs. In this system, named split-TALE (sTALE), the TALE DNA binding domain and the transcription activation domain are separated and each fused to protein interacting domains. Physical interaction of interacting domains leads to TALE-reconstitution and can be monitored by reporter gene induction. This setup was used for optimization of the sTALE scaffolds, which result in an AND-gate system with an improved signal-to-noise ratio. We also provide a toolkit of ready-to-use vectors and single modules compatible with Golden Gate cloning and MoClo syntax. In addition to its implementation in synthetic regulatory circuits, the sTALE system allows the analysis of protein-protein interactions in planta.

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Use of TALEs and TALEN Technology for Genetic Improvement of Plants

Cited by 36 publications

References 155 publications

Biotechnological Perspectives of Omics and Genetic Engineering Methods in Alfalfa

Biotechnological Perspectives of Omics and Genetic Engineering Methods in Alfalfa

Targeted Genome Editing for Cotton Improvement

Split-TALE: A TALE-Based Two-Component System for Synthetic Biology Applications in Planta

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