Targeted Genome Editing for Cotton Improvement

Khan, Zulqurnain; Khan, Sultan Habibullah; Mubarik, Muhammad Salman; Ahmad, Aftab

doi:10.5772/intechopen.73600

Cited by 10 publications

(6 citation statements)

References 150 publications

(154 reference statements)

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“…The genes of tobacco, rice, Brachypodium, barley, Arabidopsis and many other plant species have been targeted with TALEs and TAL-ENs. TALEs have been employed for suppression of cotton leaf curl virus (Khan et al 2018).…”

Section: Tales and Talens For Targeted Genome Modificationsmentioning

confidence: 99%

“…Many free web-based online softwares are available for designing of TALEs and TALENs according to the DNA sequence of choice (Khan et al 2017a, b). Several companies are providing designing and cloning services for TALEN construction on commercial basis (Khan et al 2017a, b;Khan et al 2018). Moreover, apart from nuclease domain, other effector domains are also available for TALEs which can be utilized to improve epigenetic marks and control gene expression.…”

Section: Tales and Talens For Targeted Genome Modificationsmentioning

confidence: 99%

“…Utilizing GenEd technologies, a number of organisms have already been genetically created for selective genetic modification e.g. Arabidopsis thaliana (Cermak et al 2011), tomato (Brooks et al 2014;Li et al 2018;Bari et al 2019), grapes (Ren et al 2016), potato (Butler et al 2016;Clasen et al 2016), banana (Kaur et al 2017;Kaur et al 2018;Kaur et al 2020), sorghum (Jiang et al 2013b), soybean (Curtin et al 2011;Jacobs et al 2015), maize (Liang et al 2014;Char et al 2017), cassava (Odipio et al 2017;Gomez et al 2019), Citrus sinesis (Wang et al 2019), Kiwi fruit (Wang et al 2018a, b), wheat (Wang et al 2014;Kim et al 2018), rice (Li et al 2012;Hu et al 2016;Shufen et al 2019), tobacco (Mahfouz et al 2011;Gao et al 2015), cotton (D'Halluin et al 2013;Iqbal et al 2016;Chen et al 2017;Gao et al 2017a;Wang et al 2018a, b), bacteria (Jiang et al 2013b, a), fungi (Liu et al 2017), yeast (Saccharomyces cerevisiae) (Li et al 2011), viruses (Ali et al 2015;Ji et al 2015;Khan et al 2018Khan et al , 2019, drosophila...…”

Section: Introductionmentioning

confidence: 99%

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Genome editing in cotton: challenges and opportunities

Khan

Ahmed

et al. 2023

J Cotton Res

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Cotton has enormous economic potential providing high-quality protein, oil, and fibre. A large increase in cotton output is necessary due to the world's changing climate and constantly expanding human population. In the past, conventional breeding techniques were used to introduce genes into superior cotton cultivars to increase production and to improve quality. The disadvantages of traditional breeding techniques are their time-consuming, reliance on genetic differences that are already present, and considerable backcrossing. To accomplish goals in a short amount of time, contemporary plant breeding techniques, in particular modern genome editing technologies (GETs), can be used. Numerous crop improvement initiatives have made use of GETs, such as zinc-finger nucleases, transcription-activator-like effector nucleases, clustered regularly interspaced palindromic repeats (CRISPR), and CRISPR-associated proteins systems (CRISPR/Cas)-based technologies. The CRISPR/Cas system has a lot of potential because it combines three qualities that other GETs lack: simplicity, competence, and adaptability. The CRISPR/Cas mechanism can be used to improve cotton tolerance to biotic and abiotic stresses, alter gene expression, and stack genes for critical features with little possibility of segregation. The transgene clean strategy improves CRISPR acceptability addressing regulatory issues associated with the genetically modified organisms (GMOs). The research opportunities for using the CRISPR/Cas system to address biotic and abiotic stresses, fibre quality, plant architecture and blooming, epigenetic changes, and gene stacking for commercially significant traits are highlighted in this article. Furthermore, challenges to use of CRISPR technology in cotton and its potential for the future are covered in detail.

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Section: Tales and Talens For Targeted Genome Modificationsmentioning

confidence: 99%

Section: Tales and Talens For Targeted Genome Modificationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome editing in cotton: challenges and opportunities

Khan

Ahmed

et al. 2023

J Cotton Res

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“…However, it's not always feasible due to the economic implications and the nature of the crop itself. Biological control measures, such as the use of natural enemies of the pests, have shown promise but are highly dependent on the local environmental conditions and pest population dynamics (Khan et al, 2018).…”

Section: Effectiveness Of Conventional Pest Control Measuresmentioning

confidence: 99%

Emerging Trends in CRISPR and Genetic Engineering for Enhancing Insect Tolerance in Cotton

2023

Trends Anim Plant Sci

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This review paper provides a comprehensive exploration of the application of genetic engineering and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR-Cas9) technology in enhancing insect resistance in cotton plants. With the global cotton industry facing significant threats from various pests, there is a pressing need for innovative and sustainable solutions. Genetic engineering has already made remarkable strides in this direction, with several transgenic cotton varieties demonstrating promising resistance against key pests. However, the advent of CRISPR-Cas9 has the potential to revolutionize this field, offering greater precision, efficiency, and versatility. In this review, we delve into the principles of genetic engineering and CRISPR-Cas9, outlining their mechanisms, methods, and potential in agriculture and crop improvement. We detail their roles in developing insect-resistant cotton, scrutinizing the successes, limitations, and impacts of existing genetically engineered cotton. We discuss the potential of CRISPR in creating more resilient cotton varieties, elucidating the techniques, strategies, and case studies of its application in cotton and other crops. We also delve into the regulatory and ethical considerations associated with these technologies, evaluating the current regulatory frameworks, ethical dilemmas, and public perceptions of genetically modified and CRISPR-edited crops. We then explore the potential impact of these technologies on cotton agriculture, sustainable farming, and integrated pest management, and outline future research directions and challenges. The review concludes with recommendations for future work, emphasizing the need for refining these technologies, fostering multidisciplinary research and stakeholder engagement, and addressing the associated challenges responsibly. We envisage that this exploration will contribute to the ongoing discourse and inspire future efforts in this significant field, paving the way towards a sustainable and resilient cotton industry.

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“…The CRISPR/Cas RNA-guided system is the most widely used form of GE ( Khan et al, 2018 ) and has been highly effective for site-specific genome engineering ( Peng et al, 2020 ; Zaidi et al, 2020 ). More recently, CRISPR has been used to activate (CRISPRa) or suppress (CRISPRi) genes.…”

Section: Future Directionsmentioning

confidence: 99%

The Past, Present, and Future of Host Plant Resistance in Cotton: An Australian Perspective

Egan

Stiller

2022

Front. Plant Sci.

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Cotton is a key global fiber crop. However, yield potential is limited by the presence of endemic and introduced pests and diseases. The introduction of host plant resistance (HPR), defined as the purposeful use of resistant crop cultivars to reduce the impact of pests and diseases, has been a key breeding target for the Commonwealth Scientific and Industrial Research Organisation (CSIRO) cotton breeding program. The program has seen success in releasing cultivars resistant to Bacterial blight, Verticillium wilt, Fusarium wilt, and Cotton bunchy top. However, emerging biotic threats such as Black root rot and secondary pests, are becoming more frequent in Australian cotton production systems. The uptake of tools and breeding methods, such as genomic selection, high throughput phenomics, gene editing, and landscape genomics, paired with the continued utilization of sources of resistance from Gossypium germplasm, will be critical for the future of cotton breeding. This review celebrates the success of HPR breeding activities in the CSIRO cotton breeding program and maps a pathway for the future in developing resistant cultivars.

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Targeted Genome Editing for Cotton Improvement

Cited by 10 publications

References 150 publications

Genome editing in cotton: challenges and opportunities

Genome editing in cotton: challenges and opportunities

Emerging Trends in CRISPR and Genetic Engineering for Enhancing Insect Tolerance in Cotton

The Past, Present, and Future of Host Plant Resistance in Cotton: An Australian Perspective

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