Use of Stable Sensitized Cells in Indirect Microhemagglutination Test for Malaria *

Evaluation of Stabilised Cells in the Indirect Haemagglutination Test for Echinococcosis

“…The method was modified from that of Farshy and Kagan (1972). A reaction mixture of the following substances, added in the order given, was prepared and subsequently kept in the cold: 1.7% NaC1, 12 ml; 40% v/v aqueous pyruvic aldehyde, 4 ml; 1.0% Na2C03, 35 ml; Sorensen's phosphate buffer (PH 7.2) 7 ml.…”

“…The cells were then washed thrice in PBS and stored in this as a 2.5% v/v suspension at 4°C. Double-aldehyde stabilisation ( D A S ) was performed as described by Farshy and Kagan (1972). The cells were first stabilised with pyruvic aldehyde (as above).…”

“…Earlier, Ling (1961) showed a superiority for pyruvic aldehyde and glutaraldehyde over other reagents in stabilisation of the cells. Later, Farshy and Kagan (1972) observed that cells stabilised by a combination of these two aldehydes separated by a tanning procedure were better than cells stabilised by treatment with a single aldehyde for use in IHA for malaria. The present study was undertaken to evaluate the use of Double Aldehyde Stabilised (DAS) cells and of cells stabilised by treatment with formaldehyde, pyruvic aldehyde or glutaraldehyde in an IHA test for echinococcosis.…”

“…and Kagan, 1972). Coupling agents such as aldehydes not only stabilise the cells but react with free amino groups, sulphydryl groups and, to some extent, imidazole groups, rendering the surface of the RBC more anionic and so facilitating the attachment of protein antigens to them (Farshy and Kagan, 1972). The experience of these authors with malaria, and of Jagannath and Sengupta (1981) with leprosy, showed that the use of DAS cells greatly increased the sensitivity of the IHA tests.…”

Use of Indirect Microhemagglutination Test with Stable Sensitized Red Cells in the Detection of Horse Protein Allergy

“…In the diagnosis of some infectious diseases as extraintestinal ame biasis, schistosomiasis, malaria, Pseudomonas infection or hepatitis [4][5][6][7]17], stable sensitized red blood cells have been used successfully in a standardized indirect hemagglutination test. Therefore, it was tried to develop a similar technique for the detection of antibodies against horse globulin and to compare the sensitivity and specificity of this test with the conventional indirect hemagglutination tests using fresh red blood cells as well as with other immunological methods.…”