Use of Soft X-Ray Radiography for Studying Seed Quality in Horticulture

Chavagnat, A.

doi:10.17660/actahortic.1987.215.20

Cited by 11 publications

(6 citation statements)

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“…Radiography was carried out at I.N.R.A. Angers according to the method described by Chavagnat and Le Lezec (1984) and Chavagnat (1987). Measurements of pericarp and endocarp thicknesses and true seed section were made as described by Gudin et al (unpublished).…”

mentioning

confidence: 99%

Influence of Endocarp Thickness on Rose Achene Germination: Genetic and Environmental Factors

Gudin¹,

Aréne²,

Chavagnat³

et al. 1990

HortSci

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Rose achenes of different genetic origins, all belonging to the species Rosa hybrids L., subjected to radiography films, germination tests, in situ observations of embryo development, and different temperature conditions during maturation showed that achene germination is affected by endocarp thickness. Furthermore, a relation between embryo development rate and endocarp thickness is demonstrated.

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mentioning

confidence: 99%

Influence of Endocarp Thickness on Rose Achene Germination: Genetic and Environmental Factors

Gudin¹,

Aréne²,

Chavagnat³

et al. 1990

HortSci

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“…X-ray analysis is a non-destructive, rapid method that is commonly used to analyze conifer seed health (DeBarr, 1970 ; Bramlett et al, 1977 ; Chavagnat, 1985 ; Lippitt et al, 1994 ; Machado and Cícero, 2003 ; Himanen et al, 2015 ). It has been used to study several pine species, including Pinus banksiana Lamb., Pinus elliottii Engelm., Pinus palustris Mill., Pinus ponderosa Laws., Pinus echinata Mill., Pinus taeda L., Pinus virginiana Mill., Pinus strobus L., Lithraea molleoides (Vell.)…”

Section: Introductionmentioning

confidence: 99%

Assessment and Models of Insect Damage to Cones and Seeds of Pinus strobiformis in the Sierra Madre Occidental, Mexico

et al. 2021

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Insect damage to cones and seeds has a strong impact on the regeneration of conifer forest ecosystems, with broader implications for ecological and economic services. Lack of control of insect populations can lead to important economic and environmental losses. Pinus strobiformis is the most widespread of the white pines in Mexico and is widely distributed throughout the mountains of northern Mexico. Relatively few studies have examined insect damage to the cones and seeds of these pines, especially in Mexico. In this study, we therefore analyzed insect damage to cones and seeds of P. strobiformis in Mexico by using X-ray and stereomicroscopic analysis. The specific objectives of the study were (a) to characterize insect damage by measuring external and internal cone traits, (b) to assess the health of seeds and cones of P. strobiformis in the Sierra Madre Occidental, Mexico, and (c) to estimate the relative importance of the effects of different environmental variables on cone and seed damage caused by insects. We found that 80% of P. strobiformis seeds and 100% of the tree populations studied had damage caused by insects. Most seeds were affected by Leptoglossus occidentalis, Tetyra bipunctata, Megastigmus albifrons, and the Lepidoptera complex (which includes Apolychrosis synchysis, Cydia latisigna, Eucosma bobana, and Dioryctria abietivorella). The cones of all tree populations were affected by some type of insect damage, with Lepidoptera causing most of the damage (72%), followed by Conophthorus ponderosae (15%), the hemipteran L. occidentalis (7%), and the wasp M. albifrons (6%). The proportion of incomplete seeds in P. strobiformis at the tree level, cone damage by M. albifrons and seed damage in L. occidentalis were associated with various climate and soil variables and with crown dieback. Thus, cone and seed insect damage can be severe and potentially impact seed production in P. strobiformis and the reforestation potential of the species. The study findings will enable managers to better identify insects that cause damage to cone and seeds. In addition, identification of factors associated with damage may be useful for predicting the levels of insect predation on seeds and cones.

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“… Martin et al (1998) and Gagliardi and Marcos-Filho, (2011) stated that the X-ray radiography technique is simple and that a high number of seeds can be examined in a relatively short period of time. In addition, X-ray analysis is non-destructive to the seeds ( Chavagnat, 1987 , Gagliardi and Marcos-Filho, 2011 ). Since the internal morphological structure of the seed, specifically the embryo, can be evaluated by the X-ray technique ( Van der Burg et al, 1994a , Gagliardi and Marcos-Filho, 2011 ), the relationship between germination and seed structure has been investigated for several species ( Simak, 1991 , Jorgea and Ray, 2005 , Gagliardi and Marcos-Filho, 2011 ), e.g .…”

Section: Introductionmentioning

confidence: 99%

Determination of seed viability of eight wild Saudi Arabian species by germination and X-ray tests

Al‐Turki

Baskin

2017

Saudi Journal of Biological Sciences

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Our purpose was to evaluate the usefulness of the germination vs. the X-ray test in determining the initial viability of seeds of eight wild species (, ,, ,, , and ) from Saudi Arabia. Several days were required to determine viability of all eight species via germination tests, while immediate results on filled/viable seeds were obtained with the X-ray test. Seeds of all the species, except, showed high viability in both the germination (98-70% at 25/15 °C, 93-66% at 35/25 °C) and X-ray (100-75%) test. Furthermore, there was general agreement between the germination (10% at 25/15 °C and 8% at 35/25 °C) and X-ray (5%) tests that seed viability of was very low, and X-ray analysis revealed that this was due to poor embryo development. Seeds of have physical dormancy, which was broken by scarification in concentrated sulfuric acid (10 min), and they exhibited high viability in both the germination (98% at 25/15 °C and 93% at 35/25 °C) and X-ray (98%) test. Most of the nongerminated seeds of the eight species except those of were alive as judged by the tetrazolium test (TZ). Thus, for the eight species examined, the X-ray test was a good and rapid predictor of seed viability.

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Use of Soft X-Ray Radiography for Studying Seed Quality in Horticulture

Cited by 11 publications

References 0 publications

Influence of Endocarp Thickness on Rose Achene Germination: Genetic and Environmental Factors

Influence of Endocarp Thickness on Rose Achene Germination: Genetic and Environmental Factors

Assessment and Models of Insect Damage to Cones and Seeds of Pinus strobiformis in the Sierra Madre Occidental, Mexico

Determination of seed viability of eight wild Saudi Arabian species by germination and X-ray tests

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