Determination of seed viability of eight wild Saudi Arabian species by germination and X-ray tests

Al‐Turki, Turki A.; Baskin, Carol C.

doi:10.1016/j.sjbs.2016.06.009

Cited by 35 publications

(19 citation statements)

References 25 publications

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“…Many research works have been conducted to construct potential rapid and non-destructive methods to measure seed vigor. Four non-destructive approaches with different techniques or principles, i.e., nuclear magnetic resonance spectroscopy [7], X-ray [8,9], laser speckle technique [10] and the measuring technology of seed conductivity [11] were investigated, however, they have not been widely used because of the low efficiency and complicated operation. Fortunately, recent studies show that molecular spectroscopic techniques, such as point-based and image-based hyperspectral techniques, have great potentials in the detection of seed ingredients with the advantages of high detection speed, non-destructive nature and low cost [12].…”

Section: Introductionmentioning

confidence: 99%

Rapid and Nondestructive Measurement of Rice Seed Vitality of Different Years Using Near-Infrared Hyperspectral Imaging

Feng

Sun

et al. 2019

Molecules

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Seed vitality is one of the primary determinants of high yield that directly affects the performance of seedling emergence and plant growth. However, seed vitality may be lost during storage because of unfavorable conditions, such as high moisture content and temperatures. It is therefore vital for seed companies as well as farmers to test and determine seed vitality to avoid losses of any kind before sowing. In this study, near-infrared hyperspectral imaging (NIR-HSI) combined with multiple data preprocessing methods and classification models was applied to identify the vitality of rice seeds. A total of 2400 seeds of three different years: 2015, 2016 and 2017, were evaluated. The experimental results show that the NIR-HSI technique has great potential for identifying vitality and vigor of rice seeds. When detecting the seed vitality of the three different years, the extreme learning machine model with Savitzky–Golay preprocessing could achieve a high classification accuracy of 93.67% by spectral data from only eight wavebands (992, 1012, 1119, 1167, 1305, 1402, 1629 and 1649 nm), which could be developed for a fast and cost-effective seed-sorting system for industrial online application. When identifying non-viable seeds from viable seeds of different years, the least squares support vector machine model coupled with raw data and selected wavelengths of 968, 988, 1204, 1301, 1409, 1463, 1629, 1646 and 1659 nm achieved better classification performance (94.38% accuracy), and could be adopted as an optimal combination to identify non-viable seeds from viable seeds.

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Section: Introductionmentioning

confidence: 99%

Rapid and Nondestructive Measurement of Rice Seed Vitality of Different Years Using Near-Infrared Hyperspectral Imaging

Feng

Sun

et al. 2019

Molecules

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“…Another possible combination is to use DehM instead of traditional germination method as an expediency, to testify the precision of NRSs and assist their calibration. Traditional TTC staining also complement DehM because TTC staining detects viable, non-dehiscent (dormant) seeds [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Dehiscence method: a seed-saving, quick and simple viability assessment in rice

Lin

Wang

et al. 2018

Plant Methods

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BackgroundSeed viability monitoring is very important in ex situ germplasm preservation to detect germplasm deterioration. This requires seed-, time- and labor- saving methods with high precision to assess seed germination as viability. Although the current non-invasive, rapid, sensing methods (NRSs) are time- and labor-saving, they lack the precision and simplicity which are the virtues of traditional germination. Moreover, they consume a considerable amount of seeds to adjust sensed signals to germination percentage, which disregards the seed-saving objective. This becomes particularly severe for rare or endangered species whose seeds are already scarce. Here we propose a new method that is precise, low-invasive, simple, and quick, which involves analyzing the pattern of dehiscence (seed coat rupture), followed by embryonic protrusion.ResultsDehiscence proved simple to identify. After the trial of 20 treatments from 3 rice varieties, we recognized that dehiscence percentage at the 48th hour of germination (D(48)) correlates significantly with germination rate for tested seed lots. In addition, we found that the final germination percentage corresponded to D(48) plus 5. More than 70% of the seeds survived post-dehiscence desiccation for storage. Hydrogen peroxide (1 mM) as the solution for imbibition could further improve the survival. The method also worked quicker than tetrazolium which is honored as a fast, traditional method, in detecting less vigorous but viable seeds.ConclusionWe demonstrated the comprehensive virtues of dehiscence method in assessing rice seed: it is more precise and easier to use than NRSs and is faster and more seed-saving than traditional methods. We anticipate modifications including artificial intelligence to extend our method to increasingly diverse circumstances and species.Electronic supplementary materialThe online version of this article (10.1186/s13007-018-0334-3) contains supplementary material, which is available to authorized users.

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“…X-ray images clearly showed the internal structure of the seed, including the embryo. A viable embryo has high homogeneity, but a dead one has dark heterogenous areas, indicating damage to the embryo ( Al-Turki and Baskin, 2017 , Al-Hammad and Al-Ammari, 2017 , Al-Turki et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

Ex-situ conservation of wheat genetic resources from Saudi Arabia

Al‐Turki

Al‐Namazi

Al-Ammari

et al. 2020

Saudi Journal of Biological Sciences

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Wheat ( Triticum L.) is one of the major food crops of the world, and an important component of food security. The aim of this study was to collect and preserve seeds of wheat growing in eight regions of the Kingdom of Saudi Arabia (Al-Qassim, Asir, Al-Taif, Najran, AL-Baha, Jazan, Al-Madinah and Wadi Al-Dawasir) where wheat has been cultivated since ancient times. Sixty-one accessions/samples of wheat ( Triticum aestivum ) were collected and placed in dry storage ( ex-situ conservation) at −18 °C (i.e. permanent storage). The accessions of local wheat have the ability to grow under harsh environmental conditions such as (high temperature, drought and salinity). Most of these samples were collected directly from farms, but a few were collected from markets. The most important criteria for ex-situ conservation is that seeds need to have a low moisture content (MC) and a high percentage viability. Seed MC was measured for all 61 accessions by the oven-drying method and seed viability was tested in three ways: percentage of germination, tetrazolium chloride testing, and X-ray radiography. The seed MC of the 61 accessions was uniformly very low (0.10–0.12%), and 97 to 100% of the seeds were viable. Thus, all 61 wheat accessions collected in this study have the initial requirements to remain viable for long periods of time in ex-situ conservation in the gene seed bank.

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Determination of seed viability of eight wild Saudi Arabian species by germination and X-ray tests

Cited by 35 publications

References 25 publications

Rapid and Nondestructive Measurement of Rice Seed Vitality of Different Years Using Near-Infrared Hyperspectral Imaging

Rapid and Nondestructive Measurement of Rice Seed Vitality of Different Years Using Near-Infrared Hyperspectral Imaging

Dehiscence method: a seed-saving, quick and simple viability assessment in rice

Ex-situ conservation of wheat genetic resources from Saudi Arabia

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