Use of single-layer centrifugation with Androcoll-C to enhance sperm quality in frozen-thawed dog semen

Use of Androcoll-S after thawing improves the quality of electroejaculated and epididymal sperm samples from red deer

“…The improvement in motility after selection was very high for both types of samples. These results are in accordance with other studies that showed an improvement in motility and sperm quality after selection with Androcoll in dog (Dorado et al, 2013), stallion (Macías García et al, 2009), or buck (Jiménez-Rabadán et al, 2012. Moreover, Martinez-Alborcia et al (2013) showed an improvement in sperm motility in boar after thawing, when the sperm were processed with Androcoll before freezing.…”

Effect of colloid (Androcoll-Bear, Percoll, and PureSperm) selection on the freezability of brown bear (Ursus arctos) sperm

“…Viability results also indicated that removal of dead and dying spermatozoa from the original sperm population by SLC results in better longevity of the remaining (living) spermatozoa. In dog semen [42], frozen-thawed Androcoll-C treated samples were enriched in subpopulations with highly active and PM compared with the initial samples. Percoll and PureSperm gradients have been used in the selection of sperm in several species to improve the quality of seminal samples (ram [43], marmoset [32], stallion [25,34], bull [31,44], dog [45], and human [29,46]).…”

Should single layer centrifugation of dog semen be done before or after the semen is cooled?

“…The procedure has been previously described (Dorado and others 2013c). Briefly, 0.8 ml of AND, equilibrated to room temperature (22°C), were poured into a 15 ml Falcon tube (BD Falcon Tubes, BD Biosciences, Erembodegem, Belgium).…”

“…In this sense, single layer centrifugation (SLC) has been shown to select good quality spermatozoa (Morrell and others 2009). Several colloids have been successfully used to perform SLC in different species (Morrell and others 2008, Gutiérrez-Cepeda and others 2011, Martinez-Alborcia and others 2012, Dorado and others 2013b), in particular, Androcoll-C (AND), a colloid formulation specifically for dog spermatozoa (Morrell and others 2008, Morrell and others 2013a, Dorado and others 2013c). …”