Effect of colloid (Androcoll-Bear, Percoll, and PureSperm) selection on the freezability of brown bear (Ursus arctos) sperm

Álvarez‐Rodríguez, Manuel; Álvarez, M.; Anel-López, Luis; López-Urueña, E.; Manrique, P Luis Phanor; Borragán, S.; Morrell, Jane M.; Paz, P. de; Anel, L.

doi:10.1016/j.theriogenology.2015.11.021

Cited by 15 publications

(17 citation statements)

References 52 publications

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“…To the best of our knowledge, however, no study with fresh donkey semen has been performed nor has any evaluated the effects of SLC with Equicoll on mitochondrial activity, and intracellular levels of ROS and calcium. Other studies in rams [ 14 ], pigs [ 15 ], bulls [ 16 ], and brown bears [ 17 , 18 ] have demonstrated that centrifugation with SLC increases the proportions of spermatozoa with an intact plasma membrane and acrosome, high mitochondrial activity, an intact DNA, and progressive motility, and has also been found to increase sperm cryotolerance when performed before cryopreservation [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Single Layer Centrifugation Improves the Quality of Fresh Donkey Semen and Modifies the Sperm Ability to Interact with Polymorphonuclear Neutrophils

Papas

Catalán

Recuero

et al. 2020

Animals

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This study sought to determine whether single layer centrifugation (SLC) of fresh donkey semen with Equicoll has any impact on sperm quality parameters and on the modulation of endometrial reaction following semen deposition using an in vitro model. Seventeen ejaculates from five jackasses were obtained using an artificial vagina and diluted in a skim-milk extender. Samples were either selected through SLC (Equicoll) or non-treated (control). Two experiments were performed. The first one consisted of incubating selected or non-selected spermatozoa at 38 °C for 180 min. Integrity and lipid disorder of sperm plasma membrane, mitochondrial membrane potential, and intracellular levels of calcium and reactive oxygen species were evaluated at 0, 60, 120, and 180 min. In the second experiment, polymorphonuclear neutrophils (PMN) isolated from jennies blood were mixed with selected and unselected spermatozoa. Interaction between spermatozoa and PMN was evaluated after 0, 60, 120, and 180 min of co-incubation at 38 °C. SLC-selection increased the proportions of spermatozoa with an intact plasma membrane and low lipid disorder, of spermatozoa with high mitochondrial membrane potential and with high calcium levels, and of progressively motile spermatozoa. In addition, selection through SLC augmented the proportion of phagocytosed spermatozoa, which supported the modulating role of seminal plasma proteins on sperm-PMN interaction. In conclusion, SLC of fresh donkey semen increases the proportions of functionally intact and motile spermatozoa, and appears to remove the seminal plasma proteins that inhibit sperm-PMN binding.

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Section: Introductionmentioning

confidence: 99%

Single Layer Centrifugation Improves the Quality of Fresh Donkey Semen and Modifies the Sperm Ability to Interact with Polymorphonuclear Neutrophils

Papas

Catalán

Recuero

et al. 2020

Animals

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“…Different studies have been carried out in this direction. It was found that SLC positively influenced some of the semen parameters in species and breeds such as great panda (Cai et al, 2018), brown bear (Álvarez‐Rodríguez et al, 2016; Anel‐Lopez et al, 2017), camel (Malo, Crichton, Morrell, Pukazhenthi, & Skidmore, 2017), misty panther (Tipkantha et al, 2016), andalusian donkey (Ortiz et al, 2015), red deer (Anel‐López et al, 2015) or blanca‐celtibérica goat (Jiménez‐Rabadán et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Does single‐layer centrifugation with Bovicoll improve sperm quality of frozen‐thawed semen in Fleckvieh bulls?

Szlendak

Adler

Niwinska

et al. 2020

Reprod Domestic Animals

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The aim of the present study was to evaluate the effect of sperm selection by single‐layer centrifugation (SLC) performed before freezing on sperm quality after thawing of Fleckvieh bull semen. Ejaculates from 22 bulls were collected by artificial vagina and divided into two aliquots. One aliquot (control sample) was diluted with Steridyl® and frozen over nitrogen vapour in a Digitcool freezer (IMV Technologies). Sperm from the second aliquot (SLC sample) was selected using the SLC technique with Bovicoll colloid and then frozen over nitrogen vapour in a Digitcool freezer. After thawing, both samples (control and SLC) were evaluated by computer‐aided sperm analysis (CASA; SCA 6.4 System; Microptic S.L) for sperm motility parameters. Integrity of the plasma membrane (viability), high mitochondrial membrane potential (HMMP) and acrosome integrity were assessed using a Guava® easyCyte flow cytometer (IMV Technologies). Morphological examination of spermatozoa was performed by Differential Interference Contrast microscopy (Leica DMi8). Morphological examination of live, immobilized spermatozoa was analysed under high magnification (≥6,600×). After thawing, the mean sperm viability of the control sample was 51.57%, compared to 40.37% for the SLC sample (p < .01). HMMP was higher (p < .01) in the control sample (40.37% versus 28.96%), and the mean of live spermatozoa with damaged acrosome was significantly higher (p < .03) in the SLC sample (1.63% versus 1.95%). The mean percentage of motile spermatozoa was 80.17% in the control sample, compared to 75.14% in the SLC sample (p < .0195), and rapid subpopulation reduced from 20.08% to 8.99% (p < .0001) after SLC. Percentage of hyperactivated sperm decreased from 12.23% to 4.28% (p < .0001) after SLC. Given the overall results, the sperm quality of thawed Fleckvieh bull semen was not improved when sperm were selected by SLC before freezing.

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“…The recovery rate after the sperm filter was greater than after sperm washing (89% versus 81%). [92] Turkey [93] Dog [94] Goat [69] Red deer [33] Donkey [70] Brown bear [71] Cat [53] [72] Gray wolf [74] Camel [95]…”

Section: Sperm Washingmentioning

confidence: 99%

“…There have been reports of the use of SLC to improve sperm quality in several rare species: e.g. goats [69]; donkey [70]; brown bear [71], and domestic cat as a model for wild species [72]. Use of SLC with thawed sperm samples is more appropriate for IVF than for AI in the field but is theoretically possible for the latter if there is access to a centrifuge.…”

Section: Conservation Breedingmentioning

confidence: 99%

Colloid Centrifugation of Semen: Applications in Assisted Reproduction

Morrell¹,

Rodriguez‐Martinez²

2016

AJAC

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Colloid centrifugation can be used to separate heterogeneous populations of cells particularly semen samples, which typically contain mixtures of spermatozoa at different stage of maturity together with dead and dying spermatozoa and also non-sperm cells. Colloids can be used to enrich the population of spermatozoa that are viable and functional; these are the spermatozoa that are needed for fertilization in Assisted Reproduction. Sperm samples obtained after colloid centrifugation may be enriched for motile, morphologically normal spermatozoa with intact chromatin. Insemination of mares with stallion sperm samples prepared by colloid centrifugation resulted in a higher pregnancy rate per cycle than controls, confirming that the good sperm attributes observed in the laboratory were indicative of functional spermatozoa. The method does not simply enrich for viable spermatozoa by inhibiting dead or dying spermatozoa from passing through the colloid since emerging evidence shows that colloid centrifugation can select for spermatozoa with certain properties such as metabolic activity. Other reproductive cells, such as spermatids or progenitor cells, may also be purified by colloid centrifugation. This review describes the history of colloid centrifugation for selecting spermatozoa, compares colloid centrifugation with alternative techniques, and finally describes some of the applications of the technique within the animal breeding industry.

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Effect of colloid (Androcoll-Bear, Percoll, and PureSperm) selection on the freezability of brown bear (Ursus arctos) sperm

Cited by 15 publications

References 52 publications

Single Layer Centrifugation Improves the Quality of Fresh Donkey Semen and Modifies the Sperm Ability to Interact with Polymorphonuclear Neutrophils

Single Layer Centrifugation Improves the Quality of Fresh Donkey Semen and Modifies the Sperm Ability to Interact with Polymorphonuclear Neutrophils

Does single‐layer centrifugation with Bovicoll improve sperm quality of frozen‐thawed semen in Fleckvieh bulls?

Colloid Centrifugation of Semen: Applications in Assisted Reproduction

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