Use of Salmonellae Antagonists in Fermenting Egg White

Abstract

The formation of D-homoannulated isomers during the 16a-hydroxylation of 9a-fluorohydrocortisone and other steroids by Streptomyces roseochromogenes was found to be nonbiological and to be induced by excessive iron in the medium. This iron-induced isomerization could be prevented by the addition of phosphate to the medium. Similar isomerizations during the 1,2dehydrogenation of 9a-fluoro-16a-hydroxyhydrocortisone by Bacterium cyclo-oxydans could also be prevented by the addition of phosphate. Productioni of 3-keto_-A 4-pregnadienes by Bacteriuhm cyclo-oxydans. U. S. Patent 2,822,318. M1OYER, A. J. 1953 Effect of alcohols on t;he mycological production of citric acid in surface and submerged culture. I. Nature of the alcohol effect. Appl. Microbiol.,

Use of Salmonellae Antagonists in Fermenting Egg White

“…However, replicate subcultures in brilliant green selenite broth medium were positive for Salmonella, indicating that total elimination did not occur. There are several explanations for the difference in results found in the nutrient broth (Flippin and Mickelson, 1960) and egg white cultures. The nutrient broth with glucose may provide a more ideal medium for the antagonism of Salmonellae by E. coli.…”

“…Effect of the Fermentation of Egg White by E. coli on the Survival of S. senftenberg When S. senftenberg 2623 was cultured simultaneously with E. coli 6-204-55 in nutrient broth containing glucose, there was a sharp decrease in the Salmonella population (Flippin and Mickelson, 1960). The effect was strong enough to result in the complete elimination of Salmonella from the medium in 72 hr.…”