Use of salicylate to estimate the “threshold” inducer level for <i>de novo</i> synthesis of the phenol‐degrading enzymes in <i>Pseudomonas putida</i> strain H

Janke, Dieter

doi:10.1002/jobm.3620270206

Cited by 9 publications

(2 citation statements)

References 20 publications

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“…For 1988, r2 = 0.69, y = 0.496x -0.9. pound. Janke (8) has argued that growth requirements may be the primary consideration for compounds degraded by constitutive enzymes but the concentration required for induction of enzyme synthesis is more important for other compounds. He found a phenol-oxidation induction threshold at 2 ,uM of the inducing compound.…”

Section: Discussionmentioning

confidence: 99%

Survival and Activity of a 3-Chlorobenzoate-Catabolic Genotype in a Natural System

Fulthorpe

Wyndham

1989

Appl Environ Microbiol

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A chlorobenzoate-degrading Alcaligenes strain, BR60, was introduced to flowthrough lake microcosms and exposed to 3-chlorobenzoate (3Cba) concentrations from 0 to 25 ,uM. A DNA probe specific for BR60 chlorobenzoate catabolic genes was used with the most probable number (MPN) technique to enumerate bacteria harboring this genetic information. This MPN-DNA hybridization method combined with [U-'4C]3Cba uptake rate measurements allowed the correlation of the size and activity of a specific catabolic population in a natural mixed community for the first time. An experiment involving the release of a streptomycin-resistant strain of BR60 indicated that estimates of bacteria carrying the introduced catabolic genotype often outnumbered plate count estimates of viable BR60 by as much as 3 orders of magnitude, particularly when 3Cba inputs were high. The MPN-DNA hybridization method provided catabolic population estimates highly correlated to 3Cba exposure levels and the [U-_4C]3Cba uptake rates in the microcosms. Plate counts of BR60 were poorly correlated with both 3Cba exposure levels and uptake rates. In the absence of chlorobenzoate selection, the catabolic genotype declined to very low levels by the MPN-DNA hybridization technique after 8 weeks in the microcosms.

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Section: Discussionmentioning

confidence: 99%

Survival and Activity of a 3-Chlorobenzoate-Catabolic Genotype in a Natural System

Fulthorpe

Wyndham

1989

Appl Environ Microbiol

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“…by the primary substrate or certain non-metabolizable analogous ( FEIST and HECEMAN 1969), SALA-TREPAT er al. 1972, WIGMORE et al 1977, HUGHES and BAYLY 1983, JANKE 1987, sequential enzyme induction (with the C120 being induced by the ortho-cleavage product cis, cis-muconic acid) takes place in the Fase of the ortho-pathway (STANIER and ORNSTON 1973). Typically, bacteria degrading phenol via the nwta-pathway can employ the same enzyme sequence for total degradation of the cresol isomers (RIBBONS 1970, SALA-TREPAT et (11.…”

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Enzyme regulation in in vivo‐constructed Pseudomonas putida strains with two alternative routes for oxidative degradation of phenol

Janke¹,

Kujau²,

Zippel³

et al. 1990

J. Basic Microbiol.

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Based on plate matings using P. putida strain BLlO as the recipient and either P.purida strain PG405 or PG125 as the donor, the transconjugant strains TKI and TK3 were obtained. These were shown to harbour a single plasmid of a molecular size comparable to that of PGH 1 (in the donor strain PG405) and pPGH I I (in the donor strain PG125), respectively. Clear-cut evidence was presented that a t least the transconjugant strain TK3 has the genetic potential of degrading phenol via both the or-tho-pathway (encoded by chromosomal genes) and the meta-pathway (encoded by plasmid-localized genes) due to two complete alternative enzyme sequences. However, in both transconjugant strains T K l and TK3 only the meta-pathway enzymes were found to operate during cell growth on phenol as the sole carbon source. Several lines of investigation indicated the observed predominance of the meta-pathway in T K l and TK3 cells growing on phenol lo be a specific phenomenon resulting from coordinate induction of the respective plasmid-encoded enzymes by the primary substrate.Catechol is known to be the central metabolite in oxid'ative biodegradation of numerous monocyclic aromatic compounds. In the case of phenol degradation, it is directly produced from the primary substrate due to ortko-hydroxylation by NAD(P)H-depending enzymes of the phenol 2-monooxygenase type (NEUJAHR and GAAL 1973, KRUG and STRAUBE 1986, STRAUBE 1987, ADAMS and RIBBONS 1988). Further metabolism of catechol may proceed via two alternate routes : (i) the ortho-pathway initiated by intradiolic ring-fission due to catechol 1,2-dioxygenase (C 120), (ii) the meta-pathway initiated by extradiolic ring-fission due to catechol2,3-dioxygenase (C230). These routes have been found to differ with respect to regulation of de novo synthesis of the individual degradative enzymes involved: While the rneta-pathway enzymes are subject to coordinative induction "from the top", i.e. by the primary substrate or certain non-metabolizable analogous ( FEIST and HECEMAN 1969 1987), sequential enzyme induction (with the C120 being induced by the ortho-cleavage product cis, cis-muconic acid) takes place in the Fase of the ortho-pathway (STANIER and ORNSTON 1973). Typically, bacteria degrading phenol via the nwta-pathway can employ the same enzyme sequence for total degradation of the cresol isomers (RIBBONS 1970, SALA-TREPAT et (11. 1972, BUSWELL 1975, JANKE et al. 1981. In contrast, the ortho-pathway enzymes are usually inefficient at processing total degradation of methylarenes through methylcatechols (KNACKMUSS et al. 1976).The genetic basis of oxidative phenol degradation has been examined so far only for a few Pseudornonas strains. WONG and DUNN (1976) reported Pseudomonas purida strain PP 1-2 (derived from strain ATCC 17453) to degrade phenol via the orfho-pathway by products of chromosomal genes. Recent analysis of P . putidu strain H indicated a non-conjugative 200-220 kb plasmid (pPGH1) to carry all the genes required for degradation

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In vivo generation of R68.45-pPGH1 hybrid plasmids conferring a Phl+ (meta pathway) phenotype

Herrmann

Janke²,

Krejsa

et al. 1988

Mol Gen Genet

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Plasmid pPGH1 originating from Pseudomonas putida strain H carries all the genes required for the degradation of phenol (or cresols) via the meta cleavage pathway. Besides mobilization of pPGH1 by a plasmid of the incompatibility group P-1, hybrid plasmids conferring the Phl+ phenotype could be selected, when R68.45 was the conjugative plasmid. The hybrids contain the complete R68.45 and part of pPGH1. Integration of Phl-DNA of pPGH1 into R68.45 occurred exclusively via the IS21 region of R68.45.

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Use of salicylate to estimate the “threshold” inducer level for de novo synthesis of the phenol‐degrading enzymes in Pseudomonas putida strain H

Cited by 9 publications

References 20 publications

Survival and Activity of a 3-Chlorobenzoate-Catabolic Genotype in a Natural System

Survival and Activity of a 3-Chlorobenzoate-Catabolic Genotype in a Natural System

Enzyme regulation in in vivo‐constructed Pseudomonas putida strains with two alternative routes for oxidative degradation of phenol

In vivo generation of R68.45-pPGH1 hybrid plasmids conferring a Phl+ (meta pathway) phenotype

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