Survival and Activity of a 3-Chlorobenzoate-Catabolic Genotype in a Natural System

Fulthorpe, Roberta R.; Wyndham, R. Campbell

doi:10.1128/aem.55.6.1584-1590.1989

Cited by 49 publications

(29 citation statements)

References 24 publications

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“…BR60, often failed to survive beyond three to four weeks even in the presence of chlorobenzoate, while indigenous recipients were always selected. The threshold contaminant concentration for selection of these natural recipients was 10-PM 3chlorobenzoate (Fulthorpe & Wyndham 1989). The recipient groups were not identified, and at the time we were unable to explain the determinants of fitness that allowed these recipient groups to replace the original donor.…”

Section: Resultsmentioning

confidence: 81%

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The phylogenetic distribution of a transposable dioxygenase from the Niagara River watershed

et al. 1995

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Horizontal gene transfer in the Bacteria has been demonstrated to occur under natural conditions. The ecological impact of gene transfer events depends on the new genetic material being expressed in recipient organisms, and on natural selection processes operating on these recipients. The phylogenetic distribution of cbaAB genes for chlorobenzoate 3,4-(4,5)-dioxygenase, which are carried within Tn5271 on the IncPP plasmid pBRC60, was investigated using isolates from freshwater microcosms and from the Niagara River watershed. The latter included isolates from surface water, groundwater and bioremediation reactor samples. The cbaAB genes have become integrated, through interspecific transfer, primarily into species of the p Proteobacteria (W48 isolates). Only four isolates, identified as Pseudomonas j7uorescens (3/48) and Xanthomonas maltophilia (l/48), belonged to the r Proteobacteria, despite the observation that pBRC60 was capable of mobilizing these genes into a wide range of j3 and r Proteobacteria in the laboratory. The natural host range correlated with the distribution of the meta-ring-fission pathway for metabolism of protocatechuates formed when the cbaAB genes were expressed (45/48 isolates). We proposed the hypothesis that natural selection has favoured recipients that successfully integrate the activity of the transferred dioxygenase with the conserved meta ring-fission pathway. The hypothesis was tested by transfemng a pIasmid construct containing the cbaAB genes into type strains representative of the j3 and r Proteobacteria. The concept of applying mobile catabolic genes to probe the phylogenetic distribution of compatible degradative pathways is discussed.

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Section: Resultsmentioning

confidence: 81%

“…The second was experimentally contaminated lake water and sediments which had been inoculated with Alcaligmes sp. BR60 (Fulthorpe & Wyndham 1989, 1991, 1992. Isolates were characterized initially by Gram stain and by determination of carbon source utilization with Biolog GN Miaoplates (Hayward CA).…”

Section: Bncterin and Plasmidsmentioning

confidence: 99%

The phylogenetic distribution of a transposable dioxygenase from the Niagara River watershed

et al. 1995

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“…A MPN enumeration of 2,4-~degrading microorganisms correlated well with 24-0 degradation, and both methods correlated moderately well kith colony hybridization using a gene probe specifying 2,4-~ degradation. Another significant study involved flow-through freshwater and sediment microcosms designed to closely mimic a natural shallow bay environment and measured, at different khlorobenzoate (3-CB) exposure levels, 3-CB uptake and the density of an introduced 3-CBdegrading genotype with a 3-CB gene probe (Fulthorpe & Wyndham 1989). Enrichments of water or sediment samples were set u p in a most-probable-number (MPN) format in microdilution plates containing SCB, incubated, the enriched population lysed, and the DNA hybridized to the probe.…”

Section: Dnata Rgeted Probesmentioning

confidence: 99%

Nucleic‐acid‐based methods for monitoring the performance of in situ bioremediation

Brockman

1995

Molecular Ecology

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Over the last decade, major advancements have occurred in the application of nucleicacid-based methods to detect and determine the levels of catabolic genes in environmental samples. Studies have focused on validating methods in microcosms, studying changes in the structure and expression of microbial communities in response to contaminants, and improving the sensitivity of the methods. Only in the last few years have these methods transitioned from development and validation to efforts to apply these methods for monitoring in situ bioremediation. Methods that analyse nucleic acids extracted from environmental samples are of value to bioremediation because they allow analysis independent of the artefacts that can arise from laboratory biodegradative potential assays and laboratory culture-based enumerations and from the inability to culture a large proportion of the micro-organisms in the environment. In theory, these methods enable a more comprehensive perspective, and a more defensible interpretation, of the microbial community response to intrinsic and engineered bioremediation processes. Results from the first studies applying nucleic-acid-based methods to intrinsic or engineered bioremediation indicate that these methods have both potential and limitations. The rapidly increasing number of cloned and sequenced catabolic genes, methodological advancements such as the ability to track specific micro-organisms without prior sequence data, and the potential use of bioaugmentation in the field suggest that the utility of these methods for in situ bioremediation will increase in the coming years.

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“…Since the introduced bacteria often survive at a low population density, close to, or even below the detection level, our insight in the ecology of rhizobia is still incomplete. The same holds for plant pathogens [18][19][20], human and animal pathogens [21][22][23][24][25][26], and microbes involved in the degradation of xenobiotics [27][28][29]. 187…”

Section: Why Monitoring Microbes In the Environment?mentioning

confidence: 99%

Molecular ecology of microbes: A review of promises, pitfalls and true progress

Akkermans

Mirza

Harmsen

et al. 1994

FEMS Microbiology Reviews

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Ecosystems, including engineered ones, are complex systems in which microorganisms occur in heterogenous communities. Their behaviour in the environment is often unknown due to the lack of proper detection and identification techniques. Molecular ecology is a new field in which microbes can be recognized and their function can be understood at the DNA/RNA level without unreliable steps of cultivation of microbes. During the last few years genetically modified microbes have been constructed by recombinant DNA techniques for putative use in the environment. The slow progress in this field is due to the lack of integration of microbial ecology and molecular biology. In the present review, examples will be given of the use of DNA probes and marker genes in our study on the ecology of genetically modified microbes and wild‐type recalcitrant microorganisms that are difficult to cultivate or even 'non‐culturable'. Emphasis is given to the development and use of oligonucleotide probes directed towards 16S rRNA, to detect microbes in various engineered ecosystems: (i) Frankia in root nodules, and (li) propionate‐oxidizing sulfate‐reducing bacteria in anaerobic granular sludge. Expression of genes is demonstrated by studies on the localization of nifH transcripts in root nodules of Coriaria and Alnus. In addition we will describe examples of the use of marker genes (gusA gene and aphV gene) to study competition and genetic stability of released engineered Rhizobium and Streptomyces strains.

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Survival and Activity of a 3-Chlorobenzoate-Catabolic Genotype in a Natural System

Cited by 49 publications

References 24 publications

The phylogenetic distribution of a transposable dioxygenase from the Niagara River watershed

The phylogenetic distribution of a transposable dioxygenase from the Niagara River watershed

Nucleic‐acid‐based methods for monitoring the performance of in situ bioremediation

Molecular ecology of microbes: A review of promises, pitfalls and true progress

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