Use of RP-TLC and Densitometry to Analytical Characteristic of Vitamin K₁

Abstract

Vitamin K 1 (phylloquinone) was investigated with the use of reversed phase thin layer chromatography (RP-TLC) on RP8F 254s , and RP18F 254s (E. Merck) plates, using methanol as a mobile phase. It was stated that a larger amount of the bands on a chromatogram were obtained on RP8F 254s plates than on RP18F 254s plates. Six densitometric bands on RP8F 254s plates were observed, where vitamin K 1 had R F ¼ 0.45 and k max ¼ 254 nm; however, the impurities had the R F values equal to 0.27, 0.54, 0.59, 0.62, and 0.75, respectively. It was stated that RP-TLC with densitometry and spectrodensitometry is a good method for the analysis of the qualitative composition of vitamin K 1 .

Determination of Vitamins K1, K2 MK-4, MK-7, MK-9 and D3 in Pharmaceutical Products and Dietary Supplements by TLC-Densitometry

“…In the latest available literature, only a few publications were found describing the use of the TLC method to determine vitamin K or D 3 . Pyka et al applied reversed-phase thin-layer chromatography for the analysis of vitamin K 1 purity on RP-8F 254s plates using methanol as the mobile phase [34].…”

“…Chromatographic methods are suitable and widely used for the determination of fat-soluble vitamins [29]. Thin-layer chromatography (TLC) has been applied for the determination of vitamin K 1 [34], K 2 MK-4, K 2 MK-7, and K 1 [35] and vitamin D 3 [36]. In the majority of the studies on vitamins K and D, high-performance liquid chromatography (HPLC) using different types of detection methods has been applied, including electrochemical [37], spectrophotometric, fluorimetric [38,39] or mass spectrometry/tandem mass spectrometry (LC-MS) [40][41][42].…”

Planar Chromatography

“…Bonded silica layers were used in the following analyses: color pigments in wine on C-18F with ACN−water−HCOOH (40:58:2) mobile phase ; agrimoniin and related polyphenols in pharmaceutical products on C-18W and NH 2 with diisopropyl ether−acetone−HCOOH−water (4:3:2:1), THF−ACN−water (3:1:6), and acetone−HCOOH (6:4) mobile phases ; alkaloids on CN connected to C-18 and silica plates ; and vitamin K1 composition on C-8 and C-18 plates with methanol mobile phase .…”

Rapid TLC with Densitometry for Evaluation of Naproxen Stability

“…Intensive increase developing a new drug formulation confirms that there is a big need to find a reliable, selective, sensitive, as well as cost-effective analytical method suitable for accelerated stability measurements of available pharmaceutical products. Following on our previous papers [15][16][17][18][19] and those prepared by other authors [6,[20][21][22][23]. It is well known that from all analytical methods used, thin-layer chromatography combined with densitometry (TLC-densitometry) is still simple and cost-effective stability indicating method worldwide used in pharmaceutical analysis of various active ingredients, like for example naproxen.…”