Use of response surface methodology to optimize production of pectinases by recombinant Penicillium griseoroseum T20

Gonçalves, Daniel Bonoto; Teixeira, J. A.; Bazzolli, Denise Mara Soares; Queiroz, Marisa Vieira de; Araújo, Elza Fernandes de

doi:10.1016/j.bcab.2011.09.002

Cited by 36 publications

(19 citation statements)

References 24 publications

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“…Cellulolytic MEC have attracted great attention in biofuel technology as they offer a solution to the effective degradation of complex plant materials into fermentable sugars [5]. Many fungi such as Scytalidium, Trichoderma, Aspergillus, and Penicillium have been proved effective for MEC production by agro residues fermentation [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Comparative study of multi-enzyme production from typical agro-industrial residues and ultrasound-assisted extraction of crude enzyme in fermentation with Aspergillus japonicus PJ01

Xia

Shan

et al. 2015

Bioprocess Biosyst Eng

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Submerged fermentation (SmF) and solid-state fermentation (SSF) of Aspergillus japonicus PJ01 for multi-enzyme complexes (MEC) production were comparatively studied. The results showed that orange peel and wheat bran were the best substrates for MEC production in SmF and SSF, respectively. After 72 h of cultivation under SmF, the maximal pectinase, CMCase, and xylanase activities reached 2610, 85, and 335 U/gds (units/gram dry substrate), respectively; while after 72 h of cultivation under SSF, these three enzymes' activities reached 966, 58, and 1004 U/gds, respectively. Effects of ultrasound on extraction of crude enzymes from SSF medium were determined, the maximal activities of pectinase, CMCase, and xylanase increased to 1.20, 1.48, and 1.30-fold, respectively. Apparent different mycelia growths of SSF and SmF were observed by scanning electron microscopy; and different isoforms of the crude enzyme extracts from SSF and SmF were presented by zymogram analysis.

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Section: Introductionmentioning

confidence: 99%

Comparative study of multi-enzyme production from typical agro-industrial residues and ultrasound-assisted extraction of crude enzyme in fermentation with Aspergillus japonicus PJ01

Xia

Shan

et al. 2015

Bioprocess Biosyst Eng

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“…So, the optimization of operating conditions such as pH, temperature and orbital stirring speed and nutritional factors for maximum colorants production is an essential step (Mukherjee and Singh, 2011). Comparing statistical methods and classic ones for the optimization of processes, the first represent a safe and reliable alternative because they are based on the study of only one independent variable at a time, while all of the other factors are maintained at a fixed level (Gonçalves et al , 2012). Furthermore, classical approaches are intrinsically more complicated to pinpoint interactions among processing parameters (Queiroga et al , 2012).…”

Section: Introductionmentioning

confidence: 99%

Improvement of submerged culture conditions to produce colorants by Penicillium purpurogenum

Santos-Ebinuma

Roberto

Teixeira

et al. 2014

Braz. J. Microbiol.

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Safety issues related to the employment of synthetic colorants in different industrial segments have increased the interest in the production of colorants from natural sources, such as microorganisms. Improved cultivation technologies have allowed the use of microorganisms as an alternative source of natural colorants. The objective of this work was to evaluate the influence of some factors on natural colorants production by a recently isolated from Amazon Forest, Penicillium purpurogenum DPUA 1275 employing statistical tools. To this purpose the following variables: orbital stirring speed, pH, temperature, sucrose and yeast extract concentrations and incubation time were studied through two fractional factorial, one full factorial and a central composite factorial designs. The regression analysis pointed out that sucrose and yeast extract concentrations were the variables that influenced more in colorants production. Under the best conditions (yeast extract concentration around 10 g/L and sucrose concentration of 50 g/L) an increase of 10, 33 and 23% respectively to yellow, orange and red colorants absorbance was achieved. These results show that P. purpurogenum is an alternative colorants producer and the production of these biocompounds can be improved employing statistical tool.

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“…In order to overcome this problem, optimization studies have been carried out using RSM. The theory behind RSM has been reviewed [23] [24].…”

Section: Optimization Of Degradation Of Phenolic Compounds By Rsmmentioning

confidence: 99%

Optimization of the Degradation of Hydroquinone, Resorcinol and Catechol Using Response Surface Methodology

Elboughdiri¹,

Mahjoubi²,

Shawabkeh³

et al. 2015

ACES

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A clay catalyst (montmorillonite and kaolinite) was prepared and used to degrade three phenolic compounds: hydroquinone, resorcinol and catechol obtained from the treatment the Olive Mill Wastewater (OMW) generated in the production of olive oil. The operating conditions of the degradation of these compounds are optimized by the response surface methodology (RSM) which is an experimental design used in process optimization studies. The results obtained by the catalytic tests and analyses performed by different techniques showed that the modified montmorillonites have very interesting catalytic, structural and textural properties; they are more effective for the catalytic phenolic compound degradation, they present the highest specific surface and they may support iron ions. We also determined the optimal degradation conditions by tracing the response surfaces of each compound; for example, for the catechol, the optimal conditions of degradation at pH 4 are obtained after 120 min at a concentration of H2O2 equal to 0.3 M. Of the three phenolic compounds, the kinetic degradation study revealed that the hydroquinone is the most degraded compound in the least amount of time. Finally, the rate of the catalyst iron ions release in the reaction is lower when the Fe-modified montmorillonites are used.

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Use of response surface methodology to optimize production of pectinases by recombinant Penicillium griseoroseum T20

Cited by 36 publications

References 24 publications

Comparative study of multi-enzyme production from typical agro-industrial residues and ultrasound-assisted extraction of crude enzyme in fermentation with Aspergillus japonicus PJ01

Comparative study of multi-enzyme production from typical agro-industrial residues and ultrasound-assisted extraction of crude enzyme in fermentation with Aspergillus japonicus PJ01

Improvement of submerged culture conditions to produce colorants by Penicillium purpurogenum

Optimization of the Degradation of Hydroquinone, Resorcinol and Catechol Using Response Surface Methodology

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