“…Even though the majority of these are tested and used in research with satisfactory results, they have not been included in kits for technical and economic reasons (Franco da Silveira et al 2001). Two recombinant antigens, cytoplasmic repetitive antigen (CRA) and flagellar repetitive antigen (FRA) (Lafaille et al 1989), have been deemed useful for diagnostic purposes, according to a multicentre study carried out by the WHO (Almeida et al 1990, Moncayo & Luquetti 1990, Paranhos et al 1990, Krieger et al 1992, Carvalho et al 1993. A kit for diagnosis of chronic Chagas disease called EIE-Recombinante-ChagasBiomanguinhos ® (Rec-ELISA), which uses these antigens in combination (CRA + FRA), has been developed and produced by Biomanguinhos, at the Oswaldo Cruz Foundation (Fiocruz), in Rio de Janeiro, Brazil.…”