Use of probiotics to enhance growth, stimulate immunity and confer disease resistance to Aeromonas salmonicida in rainbow trout (Oncorhynchus mykiss )

Abstract

An 8-week feeding trial was conducted to evaluate the effects of dietary probiotics on growth, nonspecific immune responses and disease resistance in juvenile rainbow trout, Oncorhynchus mykiss. Fish averaging 5.8 AE 0.8 g (mean AE SD) were fed one of the five experimental diets; one control (Cont), and four other diets were prepared by supplementing single probiotics 1 (Bacillus subtilis; SP 1 , 0.5%), single probiotics 2 (Bacillus licheniformis; SP 2 , 0.5%), multi-probiotics (B. subtilis + B. licheniformis; MP, 0.5%) and oxytetracycline (OTC) at 5 g OTC kg À1 diet. After 8 weeks of the feeding trial, weight gain and specific growth rate of fish fed SP 1 , SP 2 and OTC diets were significantly higher than those of fish fed Cont diet (P < 0.05). Superoxide dismutase (SOD) and lysozyme activities of fish fed SP 1 , SP 2 and MP diets were significantly higher than those of fish fed Cont diet (P < 0.05). There were no significant differences in SOD and lysozyme activities among fish fed SP 1 , SP 2 , MP and OTC diets. In challenge test with Aeromonas salmonicida for 15 days, fish fed SP 1 , SP 2 and MP diets showed significantly higher cumulative survival rate than those of fish fed Cont diet (P < 0.05). However, there were no significant differences in cumulative survival rate among fish fed SP 1 , SP 2 , MP and OTC diets. Although there was a little advantage in fish fed MP diet in terms of non-specific immune responses, single or multi-probiotics are equally effective statistically. These results indicate that single or multi-probiotics had equal beneficial effects as an antibiotic replacer on growth performance, non-specific immune responses and disease resistance in juvenile rainbow trout.

Effects of dietaryLactobacillus delbrueckiion growth performance, body composition, digestive and absorptive capacity, and gene expression of common carp (Cyprinus carpioHuanghe var)

“…The present study reported that supplementation of L. delbrueckii in the diet resulted in improved growth performance of Cyprinus carpio Huanghe var. Similar benefits were previously reported in red sea bream ( Pagrus major ) (Dawood et al, ), sea cucumber ( Apostichopus japonicas ) (Zhao, Zhang, et al, ), olive flounder ( Paralichthys olivaceus ) (Beck et al, ), rainbow trout ( Oncorhynchus mykiss ) (Park et al, ), Juvenile Chitala chitala (Mitra, Mukhopadhyay, & Homechaudhuri, ) and Totoaba macdonaldi (González‐Félix et al, ). According to previous studies, it has been suggested that the enhanced growth performance of fish fed dietary L. delbrueckii might be attributed to the elevated health status, digestibility, absorptivity and stimulation of enzymatic secretion.…”

Shortening Fermentation Period and Quality Improvement of Fermented Fish, Chouguiyu, by Co-inoculation of Lactococcus lactis M10 and Weissella cibaria M3

“…For the W group, strains such as Aeromonas were detected in addition to W. cibaria M3. A. salmonicida (Park et al., 2017) and Streptococcus parauberis (Fernandez-No et al., 2012) were reported as the pathogenic bacteria in aquatic products and were commonly found in food spoilage. The reason for more spoilage bacteria found in group W may be due to the weak ability of W. cibaria M3 to produce bacteriocin and its limited ability to inhibit other microorganisms.…”

Evaluation of dietary natural mineral materials as an antibiotic replacer on growth performance, non-specific immune responses and disease resistance in rainbow trout,Oncorhynchus mykiss

“…In a previous study, Lee, Katya, Yun, Yoon, Park, Sung, Shin and Bai () reported that dietary yellow loess at 1–2% per kg diet (0.2–0.4% on dry matter basis) has positive impact as an antibiotic replacer in terms of growth improvement and disease resistance in juvenile rainbow trout. Dietary additive studies in juvenile fish were usually conducted for 1–8 weeks (Sharifuzzaman & Austin ; Park, Lee, Hong, Kim, Moniruzzaman & Bai ), whereas in larger fish the experiment lasted for more weeks to understand the effects of additives in fish (Shin, Cha, Rahimnejad, Jeong, Yoo, Lee, Ahn, Choi, Choi, Park, Kim & Lee ). However, the reasons are not clearly ascertained (Sharifuzzaman & Austin ).…”