Use of Polyurethane Minisponges to Collect Human Tear Fluid

López-Cisternas, Juan; Castillo-Díaz, Jessica; Traipe-Castro, Leonidas; López‐Solís, Remigio

doi:10.1097/01.ico.0000183531.25201.0d

Cited by 40 publications

(42 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A theoretical advantage of the capillaries compared to the other methods is that it can collect the pure tear. Nevertheless, López-Cisternas et al [26] have demonstrated that the results of the electrophoretic protein profiles of tear samples were indistinguishable between polyurethane foam cylinders (similar material to polyvinyl acetal) and to the one obtained with capillaries.…”

Section: Discussionmentioning

confidence: 99%

Inflammatory Markers in the Tears of Patients with Ocular Surface Disease

et al. 2008

View full text Add to dashboard Cite

Purpose: To determine the concentration of interleukins (IL-1β and -6) and matrix metalloproteinase 9 (pro-MMP-9) in the tears of patients with different ocular surface diseases and to examine the possible relationship between the disorders and molecular inflammation. Methods: 77 patients diagnosed as having different ocular surface disorders and 18 normal control subjects were studied. Patients were routinely examined and separated into 5 groups: (1) control, (2) blepharitis, (3) ocular allergic disease, (4) dry eye and (5) conjunctivochalasis. Ten microliters of tears were collected by a Weck cell sponge. The concentrations of IL-1β, IL-6 and pro-MMP-9 were measured by enzyme-linked immunosorbent assay, and the MMP-9 activity was evaluated with gelatin zymography. Results: Levels of IL-1β and IL-6 in tear fluid were significantly higher in conjunctivochalasis (p = 0.0062 and p = 0.0134) than in the control group. Pro-MMP-9 levels were significantly elevated in blepharitis (p = 0.013), in allergic eye disease, in dry eye and in conjunctivochalasis (all p < 0.001), in comparison to controls. Conclusions: Pro-MMP-9 levels in tears are elevated in all of the studied pathologies especially in ocular allergy and conjunctivochalasis. However, IL-1β and IL-6 were only found to be overexpressed in conjunctivochalasis. These findings illustrate the selective implication of different molecules in each disorder.

show abstract

Section: Discussionmentioning

confidence: 99%

Inflammatory Markers in the Tears of Patients with Ocular Surface Disease

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Microsponges were then centrifuged to dryness at 13,000 rpm for 3 minutes to recover tears [4]. The amount of fluid recovered was determined as previously described [12]. Briefly, it was obtained by weighing the sponge and the tube after absorption and centrifugation steps, respectively.…”

Section: Methodsmentioning

confidence: 99%

MUC5AC overexpression in tear film of neonates

Mantelli

Tiberi

Micera

et al. 2007

Graefes Arch Clin Exp Ophthalmol

View full text Add to dashboard Cite

As far as we are aware this is the first description of the mucus tear-film layer in neonates. The greater tear-film stability in neonates has been so far attributed to a thicker lipid layer. In our study, we show that a concomitant increase in MUC5AC protein expression in tears is present and may contribute to this greater stability; therefore, both mucus and lipid layer should be considered while evaluating tear film stability in neonates.

show abstract

“…We investigated the tear protein pattern as obtained by varying concentrations of polyacrylamide gels, and compared with the results of other published studies [19,20,24,32,33]. We identified a commercially available protein standard marker, SBTI, with a molecular weight of 20.1 kD, that did not overlap with any of the major tear protein bands in the 15% gel (Fig.…”

Section: The Choice Of the Internal Standard Proteinmentioning

confidence: 98%