Quantification of tear proteins by SDS-PAGE with an internal standard protein: A new method with special reference to small volume tears

Abstract

Using SBTI as an internal standard, we can simultaneously quantify total tear protein and the major tear protein components by SDS-PAGE densitometry using small volume tears. This method appears promising for use as a diagnostic tool for identifying the occurrence of ocular diseases with tear protein changes.

<p>Effectiveness of prophylactic intravitreal bevacizumab injection to proliferative diabetic retinopathy patients with elevated preoperative intraocular VEGF in preventing complications after vitrectomy</p>

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<p>Effectiveness of prophylactic intravitreal bevacizumab injection to proliferative diabetic retinopathy patients with elevated preoperative intraocular VEGF in preventing complications after vitrectomy</p>

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Effect of tear collection on lacrimal total protein content in dogs and cats: a comparison between Schirmer strips and ophthalmic sponges

“…Consistent with older reports in human subjects, the higher TPC in absorbent materials such as Schirmer strips vs. capillary tubes is presumably related to an increased in serum-derived proteins due to conjunctival irritation [ 7 , 17 ]. However, these findings are not consistent across the scientific literature as recent studies have described an equal [ 9 ] or inferior [ 18 ] amount of proteins in Schirmer-collected tears when compared to capillary tubes. Thus, it is likely inaccurate to compare the findings of various studies based on the tear collection method alone.…”

Impact of diurnal variation, sex, tear collection method, and disease state on tear protein levels in dogs

“…Here, we showed that Schirmer strips compared well with capillary glass tubes except for minor discrepancies such as serum albumin levels at a single time point (9 am) and TPC in eyes with moderate conjunctivitis. Similar comparisons yielded conflicting results in the human literature with either higher, 35 equal, 36 or inferior 37 amounts of proteins in Schirmer-collected tears when compared to capillary tubes. Further, disease state did not exacerbate differences between tear collection methods in canine eyes, in contrast to the hypothesis that Schirmer strips might capture larger protein amounts by causing micro-trauma to the hyperemic and more vulnerable conjunctiva.…”

<p>Effectiveness of prophylactic intravitreal bevacizumab injection to proliferative diabetic retinopathy patients with elevated preoperative intraocular VEGF in preventing complications after vitrectomy</p>

“……”

“……”

Effect of tear collection on lacrimal total protein content in dogs and cats: a comparison between Schirmer strips and ophthalmic sponges

“…Consistent with older reports in human subjects, the higher TPC in absorbent materials such as Schirmer strips vs. capillary tubes is presumably related to an increased in serum-derived proteins due to conjunctival irritation [ 7 , 17 ]. However, these findings are not consistent across the scientific literature as recent studies have described an equal [ 9 ] or inferior [ 18 ] amount of proteins in Schirmer-collected tears when compared to capillary tubes. Thus, it is likely inaccurate to compare the findings of various studies based on the tear collection method alone.…”

Impact of diurnal variation, sex, tear collection method, and disease state on tear protein levels in dogs

“…Here, we showed that Schirmer strips compared well with capillary glass tubes except for minor discrepancies such as serum albumin levels at a single time point (9 am) and TPC in eyes with moderate conjunctivitis. Similar comparisons yielded conflicting results in the human literature with either higher, 35 equal, 36 or inferior 37 amounts of proteins in Schirmer-collected tears when compared to capillary tubes. Further, disease state did not exacerbate differences between tear collection methods in canine eyes, in contrast to the hypothesis that Schirmer strips might capture larger protein amounts by causing micro-trauma to the hyperemic and more vulnerable conjunctiva.…”

<p>Effectiveness of prophylactic intravitreal bevacizumab injection to proliferative diabetic retinopathy patients with elevated preoperative intraocular VEGF in preventing complications after vitrectomy</p>

“……”

“……”

Effect of tear collection on lacrimal total protein content in dogs and cats: a comparison between Schirmer strips and ophthalmic sponges

“…Consistent with older reports in human subjects, the higher TPC in absorbent materials such as Schirmer strips vs. capillary tubes is presumably related to an increased in serum-derived proteins due to conjunctival irritation [ 7 , 17 ]. However, these findings are not consistent across the scientific literature as recent studies have described an equal [ 9 ] or inferior [ 18 ] amount of proteins in Schirmer-collected tears when compared to capillary tubes. Thus, it is likely inaccurate to compare the findings of various studies based on the tear collection method alone.…”

Impact of diurnal variation, sex, tear collection method, and disease state on tear protein levels in dogs

“…Here, we showed that Schirmer strips compared well with capillary glass tubes except for minor discrepancies such as serum albumin levels at a single time point (9 am) and TPC in eyes with moderate conjunctivitis. Similar comparisons yielded conflicting results in the human literature with either higher, 35 equal, 36 or inferior 37 amounts of proteins in Schirmer-collected tears when compared to capillary tubes. Further, disease state did not exacerbate differences between tear collection methods in canine eyes, in contrast to the hypothesis that Schirmer strips might capture larger protein amounts by causing micro-trauma to the hyperemic and more vulnerable conjunctiva.…”