Use of piezoelectric shock waves to effect release of amylase from capsules containing the amylase producing rat pancreatic tumour cell line AR42J

Jones, Bleddyn; McCarthy, Una; Bunni, L.; McHale, L.; Butler, Michael R.; Seary, J.; McHale, Anthony P.

doi:10.1007/bf01875636

Cited by 4 publications

(2 citation statements)

References 6 publications

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“…In addition, since, in the above described strategy, the enzyme -galactosidase was immobilized to the magnetically responsive particles, the enzyme preparation could be retained when the function of the yeast component became exhausted. This could be accomplished by re-solubilization of the alginate [10] and subsequent retention of the magnetically responsive particles upon solubilization of that matrix. The yeast could be disposed of and both the immobilized enzyme and possibly the recovered alginate could be re-used in production of more biocatalyst.…”

Section: Resultsmentioning

confidence: 99%

The effect of Mn2+ on ethanol production from lactose using Kluyveromyces marxianus IMB3 immobilized in magnetically responsive matrices

Brady¹,

Nigam²,

Marchant³

et al. 1997

Bioprocess Engineering

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The thermotolerant, ethanol producing yeast strain, K. marxianus IMB3 was immobilized in calcium alginate containing magnetically responsive Fe 3 O 4 particles. In these studies the -galactosidase derived from K. marxianus IMB3 was immobilized onto the Fe 3 O 4 particles prior to inclusion into the alginate matrix. Ethanol production by the immobilized microorganism in the presence of Fe 3 O 4 reached a maximum of 16 g/L on 40 g/L lactose whereas prior immobilization of the enzyme to the particles and inclusion into the alginate matrix increased ethanol production to a maximum concentration of 18 g/L. When Mn 2+ was incorporated into fermentations containing the immobilized enzyme in the alginate matrix, ethanol production increased further to a maximum concentration of 20 g/L. In addition, the behaviour of the magnetically responsive biocatalyst containing the co-immobilized enzyme was examined in a batch-fed system in the presence and absence of Mn 2+ .

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Section: Resultsmentioning

confidence: 99%

The effect of Mn2+ on ethanol production from lactose using Kluyveromyces marxianus IMB3 immobilized in magnetically responsive matrices

Brady¹,

Nigam²,

Marchant³

et al. 1997

Bioprocess Engineering

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“…The aim of this article is to emphasize the significance of the use of polymers in cell preparations and to demonstrate the differences between encapsulation or entrapment and a novel single-cell coating technology. In recent years a significant progress has been observed in the trend to use prokaryotic and eukaryotic cells for the production of extracellular molecules such as antibiotics and hormones [18,19], and in the design of artificial cells and organs [20,21]. These systems include the entrapment of cells in polymeric gel matrix, the attachment of cells to carriers and encapsulation of cells in small capsules or hollow fibers.…”

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confidence: 99%

The use of polymers for coating of cells

Kampf

2002

Polymers for Advanced Techs

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Bare cells or tissues are sometimes exposed to external hazardous conditions, physical (thermal changes), chemical or biological (such as enzymes, bacteria and viruses). Liquids, semi‐liquids (e.g. gels) or solid films are often used to surround individual cells or tissues in order to protect them and to achieve greater stability in laboratory and industrial uses. On the one hand there is a need for isolation and creating a barrier between the cell and its surroundings, and on the other hand this should not affect the metabolism and viability of the cell used. Polymers are major substances for cell encapsulation and entrapment. These preparations are often used for the manufacture of drugs and other cell metabolites. There are only a few publications describing single‐cell coating. We can consider a definition of coating as a process of surrounding an object with a thin film, having a thickness of only a small fraction of the object's dimensions. Taking this definition into account, in fact, most coating experiments actually deal with entrapment or immobilization rather than coating. The latest research shows for the first time the ability to coat a single cell by producing a thin gel layer around it. Xenopus laevis eggs and embryos were used as a model system for coating with thin films composed of low‐methoxy pectin (LMP), alginate, and ι‐ and κ‐carrageenans. These gums have different compositions and structures and accordingly created different coatings around the cells. The first goal was to achieve a thin film (∼50 µm) around an individual non‐fertilized egg (one cell). Next, the influence of coating an early‐stage toad embryo was tested. All coated embryos appeared to develop normally, similar to non‐coated embryos. The survival percentages after hatching of coated embryos were significantly higher than of the non‐coated control. In addition, the coating served as a barrier to microbial contamination and thus improved survival prospects. The coatings delayed hatching by 18 to 24 hr. At hatch, the embryos were at a more developed stage than their non‐coated counterparts. Thus, coating cells by polymeric thin film appears to be a suitable tool for laboratories interested in performing longer‐term experiments with cells or embryos. Copyright © 2003 John Wiley & Sons, Ltd.

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Molecular cloning and functional expression of a Talaromyces emersonii derived alpha-amylase encoding genetic determinant in a human cell line

Bunni

Hackett

McHale³

et al. 1993

Biotechnol Lett

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A eDNA fragment encoding a Talaromyces emersonii acid stable alpha-amylase has beun cloned into a mammalian cell expression vector system. When human HeLa cells were tnmsformed with this DNA, functional enzyme could be detected in extracellular media and cell lysates derived from stable transfonnants. Expression of the T. emersonii derived eDNA w a s verified by northern dot blotting hyl~'idi~tion analysis of mRNA exUacted from mmsformants, using the labelled amylase encoding eDNA determinant as a probe. Results obtained suggest that expression of the fungus derived alpha-amylase in the transformed HeLa cells is glucocorticoid dependent.

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Use of piezoelectric shock waves to effect release of amylase from capsules containing the amylase producing rat pancreatic tumour cell line AR42J

Cited by 4 publications

References 6 publications

The effect of Mn2+ on ethanol production from lactose using Kluyveromyces marxianus IMB3 immobilized in magnetically responsive matrices

The effect of Mn2+ on ethanol production from lactose using Kluyveromyces marxianus IMB3 immobilized in magnetically responsive matrices

The use of polymers for coating of cells

Molecular cloning and functional expression of a Talaromyces emersonii derived alpha-amylase encoding genetic determinant in a human cell line

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