Abstract:A eDNA fragment encoding a Talaromyces emersonii acid stable alpha-amylase has beun cloned into a mammalian cell expression vector system. When human HeLa cells were tnmsformed with this DNA, functional enzyme could be detected in extracellular media and cell lysates derived from stable transfonnants. Expression of the T. emersonii derived eDNA w a s verified by northern dot blotting hyl~'idi~tion analysis of mRNA exUacted from mmsformants, using the labelled amylase encoding eDNA determinant as a probe. Resul… Show more
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