Use of partially purified peroxidase of agricultural by‐product rice bran in deoxynivalenol reduction

Gautério, Gabrielle Victória; Malta, Danielle Specht; Reginatto, Laís; Feltrin, Ana Carla Penteado; Garda‐Buffon, Jaqueline; Kalil, Susana Juliano

doi:10.1002/jctb.5191

Cited by 15 publications

(5 citation statements)

References 70 publications

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“…The reaction time for both systems, crude enzyme extract and purified, was 10 min. Gautério et al [35] also used the enzyme peroxidase purified from rice bran and found a DON degradation of 82% in the model system. The authors [35] emphasise that oxidative activity of the enzyme may not be the only mechanism to reduce the DON concentration and that proteins may exhibit adsorption capacity due to the active groups on the surface of the protein, which may occur in substitution or in conjunction with an oxidative mechanism.…”

Section: Reduction Of Ota Levelsmentioning

confidence: 99%

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Ochratoxin A reduction by peroxidase in a model system and grape juice

et al. 2019

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This study aimed at evaluating the potential of the peroxidase (PO) enzyme to reduce ochratoxin A (OTA) levels and its application to grape juice. Both commercial PO and PO extracted from rice bran were evaluated, respectively, regarding their activity towards OTA in a model system. The affinity between PO and OTA was verified by the Michaelis-Menton constant and the maximum velocity parameters, resulting in 0.27 μM and 0.015 μM min −1 for the commercial enzyme, and 6.5 μM and 0.031 μM min −1 for PO extracted from rice bran, respectively. The lowest residual OTA levels occurred when 0.063 U mL −1 of the enzyme was applied. Under these conditions, the OTA reduction was 41% in 5 h for the commercial enzyme, and 59% in 24 h, for PO extracted from rice bran. When the extracted PO, with the activity of 0.063 U mL −1 , was applied to whole grape juice, the OTA levels decreased to 17%, at 24 h. The capacity shown by PO for reducing OTA levels was confirmed in whole white grape juice, as a model system. This study may assist the wine industry to offer healthier products and add value to rice bran.

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Section: Reduction Of Ota Levelsmentioning

confidence: 99%

“…Gautério et al [35] reported a reduction of 81.7% DON in a model system, using PO also purified from rice bran. The authors emphasise that they cannot affirm whether there is an oxidative action of the enzyme in the trichothecene structure.…”

Section: Ota Concentration Reduction Mechanismmentioning

confidence: 99%

Ochratoxin A reduction by peroxidase in a model system and grape juice

et al. 2019

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“…Another study was performed by Gautério et al, 45 where the peroxidase enzyme obtained and purified from rice bran was used in a model system resulting in a reduction of 81.7% of DON. The authors emphasize that they cannot affirm whether there is actually an oxidative action of the enzyme on the trichothecene structure.…”

Section: Trichothecene a And B Biodegradationmentioning

confidence: 99%

Evaluation of the Suitability of Analytical Methods in Trichothecene A and B Degradation

Feltrin

Sibaja

Tusnski

et al. 2018

J. Braz. Chem. Soc.

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An investigation was conducted to standardize conditions for laccase and lipase activity required to maximize trichothecene degradation. The analytical methods were suitable and validated in model solutions for detection of five analytes (T-2 toxin, deoxynivalenol, nivalenol, 3-acetyldeoxynivalenol and 15-acetyldeoxynivalenol), the methods were then applied in the degradation tests of these trichothecenes. The method of salting-out assisted liquid-liquid extraction (SALLE), using three different NaCl concentration levels, obtained recoveries in the range of 47.4-103.4% at pH 5.0 and 36.6-106.8% at pH 7.0, with an intra-day relative standard deviation under 15% for the majority of the compounds. Quantification limits remained in the region of 0.07 μg mL -1 for 15-acetyldeoxynivalenol and 0.3 μg mL -1 for nivalenol. Finally, the suitable analytical methods were applied in a study of trichothecene degradation by enzymatic action, resulting in a reduction of 12.3% for T-2 toxin, 68.4% for deoxynivalenol, 50.2% for 3-acetyldeoxynivalenol and 45.4% for 15-acetyldeoxynivalenol.

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“…Among enzymes, commercial peroxidase (PO) has shown that it is effective at mitigating mycotoxins in different feed matrices [27,28]. Other studies have shown that PO obtained from plant extracts (e.g., soy and rice bran) is effective at mitigating mycotoxins [23,29,30], and depending on the materials to which it is applied, it may reach better results than commercial enzymes [24]. However, there is a lack of studies describing how application of PO to animal diets to inactivate fungal contaminants can interact with other nutrients and active compounds, affecting their potential bioavailability and influencing both the metabolism of the species and the quality of the final product [31].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Interactions of Added Soybean Peroxidase with Other Nutrients Present in Fish Feeds Using an In Vitro Digestive Simulation

Nogueira,

Aznar-García,

Martínez-Antequera

et al. 2023

Animals

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Peroxidase (PO) has been applied in different areas of industrial biotechnology, including the control of contaminants like aflatoxin B1 in fish feeds. However, its potential negative interactions with the macro and micro components of feeds have not been evaluated. The aim of this study was to evaluate the impact of PO’s addition to a feed on compounds like fatty acids and polyphenols using an in vitro simulation of the digestive tract of the tilapia. The influence on fatty acids was determined by changes in the peroxide index, with the feed including PO presenting values four times higher than those of the control feed. On the other hand, the in vitro digestive simulation also evidenced an effect of PO on the bioaccessibility of polyphenols significantly influenced by the total digestion time and temperature. The bioaccessibility of polyphenol ranged from 2.09 to 16.23 μmol of the total Trolox equivalent antioxidant capacity for the combinations evaluated in the study. The greatest bioaccessibility was observed at the central point under the following conditions of digestive hydrolysis: pH of 7, 30 °C, 4.5 h of digestive hydrolysis and an absence of PO.

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Use of partially purified peroxidase of agricultural by‐product rice bran in deoxynivalenol reduction

Cited by 15 publications

References 70 publications

Ochratoxin A reduction by peroxidase in a model system and grape juice

Ochratoxin A reduction by peroxidase in a model system and grape juice

Evaluation of the Suitability of Analytical Methods in Trichothecene A and B Degradation

Evaluation of Interactions of Added Soybean Peroxidase with Other Nutrients Present in Fish Feeds Using an In Vitro Digestive Simulation

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