Use of one- and two-mediator systems for developing a BOD biosensor based on the yeast Debaryomyces hansenii

Зайцева, Анна Сергеевна; Arlyapov, V. A.; Yudina, N.Yu.; Alferov, S. V.; Reshetilov, A. N.

doi:10.1016/j.enzmictec.2016.12.005

Cited by 30 publications

(12 citation statements)

References 31 publications

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“…147 The chemiluminescence property is used as a signal for the BOD detection by using mediators (ferrocene-neutral red) and D. Hansenii yeast cells for designing an amperometric graphite paste electrode. 148 The amount of BOD detected was 19.5 mg O 2 L −1 in wastewater.…”

Section: ■ Micro Mfc-based Bod Sensorsmentioning

confidence: 92%

“…Thus, a two-mediator system (H 2 O 2 –vitamin K3) based optical biosensor was designed using yeast ( S. cerevisiae ) . The chemiluminescence property is used as a signal for the BOD detection by using mediators (ferrocene-neutral red) and D. Hansenii yeast cells for designing an amperometric graphite paste electrode . The amount of BOD detected was 19.5 mg O 2 L –1 in wastewater.…”

Section: Comparative Study On Mfc and Other Biosensors For The Detect...mentioning

confidence: 99%

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Microbial Fuel Cell-Based Biological Oxygen Demand Sensors for Monitoring Wastewater: State-of-the-Art and Practical Applications

2020

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Environmental pollution has been a continuous threat to sustainable development and global well-being. It has become a significant concern worldwide to combat the ecological crisis using low-cost innovative technologies. Biological oxygen demand (BOD) is a key indicator to comprehend the quality of water to guarantee environmental safety and human health; however, none of the present technologies are capable of online monitoring of the water at the source. Microbial fuel cells (MFC) are a promising technology for simultaneous power generation and wastewater treatment. MFCs have also been shown in fascinating applications to measure and detect the toxic pollutants present in wastewater. These are the bioreactors where exoelectrogenic microorganisms catalyze the conversion of the inherent chemical energy stored in organic compounds to electrical energy. Sensors employ energy conversion to measure BOD, which is considered an international index for the detection of organic material load present in wastewater. The MFC-based BOD sensors have gone through a wide range of advancement from mediator to mediator-less, double chamber to single-chamber, and large size to miniature. There have been detailed studies to improve the accuracy and reproducibility of the sensors for commercial applications. Additionally, multistage MFC-based BOD biosensors and miniature MFC−BOD sensors have also been ubiquitous in recent years. A considerable amount of work has been carried out to improve the performance of these devices by fabricating the proton exchange membranes and altering catalysts at the cathode. However, there remains a dearth for the fabrication of the devices in aspects like suitable microbes, proton exchange membranes, and cheaper catalysts for cathodes for effective real-time monitoring of wastewater. In this review, an extensive study has been carried out on various MFC-based BOD sensors. The efficiency and drawbacks associated with the different MFC-based BOD sensors have been critically evaluated, and future perspectives for their development have been investigated. The breadth of work compiled in this review will accelerate further research in MFC-based BOD biosensors. It will be of great importance to broad ranges of scientific research and industry.

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Section: ■ Micro Mfc-based Bod Sensorsmentioning

confidence: 92%

Section: Comparative Study On Mfc and Other Biosensors For The Detect...mentioning

confidence: 99%

Microbial Fuel Cell-Based Biological Oxygen Demand Sensors for Monitoring Wastewater: State-of-the-Art and Practical Applications

2020

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“…The calibration dependence of the response R of the untreated and DND modified materials on the glucose concentration is shown in Figure 9a. Since the whole cells receptor elements are actually the catalytic type bioreceptors [39,40], the dependencies are fit by the following Michaelis-Menten equation:…”

Section: In Vivo Experimentsmentioning

confidence: 99%

Modified Nanodiamonds as a Means of Polymer Surface Functionalization. From Fouling Suppression to Biosensor Design

et al. 2021

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The development of different methods for tuning surface properties is currently of great interest. The presented work is devoted to the use of modified nanodiamonds to control the wetting and biological fouling of polymers using optical sensors as an example. We have shown that, depending on the type of modification and the amount of nanodiamonds, the surface of the same fluorinated polymer can have both bactericidal properties and, on the contrary, good adhesion to the biomaterial. The precise control of wetting and biofouling properties of the surface was achieved by the optimization of the modified nanodiamonds thermal anchoring conditions. In vitro and in vivo tests have shown that the fixation of amine functional groups leads to inhibition of biological activity, while the presence of a large number of polar groups of mixed composition (amide and acid chloride) promotes adhesion of the biomaterial and allows one to create a biosensor on-site. A comprehensive study made it possible to establish that in the first 5 days the observed biosensor response is provided by cells adhered to the surface due to the cell wall interaction. On the 7th day, the cells are fixed by means of the polysaccharide matrix, which provides much better retention on the surface and a noticeably greater response to substrate injections. Nevertheless, it is important to note that even 1.5 h of incubation is sufficient for the formation of the reliable bioreceptor on the surface with the modified nanodiamonds. The approach demonstrated in this work makes it possible to easily and quickly isolate the microbiome on the surface of the sensor and perform the necessary studies of its substrate specificity or resistance to toxic effects.

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“…To date, tremendous efforts have been devoted to developing new BOD detection methods to reduce assay time and simplify procedures to meet the requirements of end users [4,7,[9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26], such as (i) biosensors based on biolumines-cent bacteria, (ii) biosensors with redox mediators, (iii) microbial fuel cell (MFC) biosensor, (iv) biosensors with entrapped microorganisms, and (v) biosensors based on the bioreactor/chemist at technology [2,5]. Among them, because of the complexity and low precision of the bioluminescence reaction, the two former technologies show the least potential in practical applications [5,20,22,23,27,28]. Currently, the most widely adopted approach is the type of biosensor method based on the respiration rate [5,26].…”

Section: Introductionmentioning

confidence: 99%

A Current Sensing Biosensor for BOD Rapid Measurement

Liu

Nianxin

et al. 2020

Archaea

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In order to improve the practicality of the rapid biochemical oxygen demand (BOD) method, a highly sensitive rapid detection method for BOD that is based on establishing the correlation between current and dissolved oxygen (DO) was developed. In this experiment, Bacillus subtilis was used as the test microorganism, and the embedding method was used to achieve quantitative fixation of microorganisms, which could increase the content of microorganisms and prolong the service life of the biological element. The conductivity (COND) probe is used as a sensing element, so that the testing value can be read every second. In the program, the moving average method is used to process the collected data so that the value can be read every minute. National standard samples were detected to test the accuracy and stability of the method. The results showed that relative error and analytical standard deviations were less than 5%. Different polluted water was tested to evaluate its application range. The results showed that relative error was less than 5%. The results of the method are consistent with the results of the wastewater sample obtained by the BOD5 standard method. The proposed rapid BOD current sensing biosensor method should be promising in practical application of wastewater monitoring.

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Use of one- and two-mediator systems for developing a BOD biosensor based on the yeast Debaryomyces hansenii

Cited by 30 publications

References 31 publications

Microbial Fuel Cell-Based Biological Oxygen Demand Sensors for Monitoring Wastewater: State-of-the-Art and Practical Applications

Microbial Fuel Cell-Based Biological Oxygen Demand Sensors for Monitoring Wastewater: State-of-the-Art and Practical Applications

Modified Nanodiamonds as a Means of Polymer Surface Functionalization. From Fouling Suppression to Biosensor Design

A Current Sensing Biosensor for BOD Rapid Measurement

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