Use of Non-Cross-Linked Polyacrylamide for Four-Color DNA Sequencing by Capillary Electrophoresis Separation of Fragments up to 640 Bases in Length in Two Hours

Zhang, JianZhong.; Fang, Yu; Hou, Joan Y.; Ren, Hong Ji.; Jiang, Rong; Roos, Pieter; Dovic̀hi, Norman J.

doi:10.1021/ac00120a026

Cited by 124 publications

(90 citation statements)

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“…The large surface to volume ratio of the capillary permits higher electric fields that result in efficient and rapid separation of products. [29] Some groups have used non-crosslinked polymer-filled capillaries for restriction fragment analysis [30] and DNA sequencing [31][32][33]. This technology allows replacement of separation matrix after each run permitting more runs per capillary, automation, and easier usage.…”

Section: Post-pcr Detectionmentioning

confidence: 99%

Flow through PCR module of BioBriefcase

et al. 2005

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The BioBriefcase is an integrated briefcase-sized aerosol collection and analysis system for autonomous monitoring of the environment, which is currently being jointly developed by Lawrence Livermore and Sandia National Laboratories. This poster presents results from the polymerase chain reaction (PCR) module of the system. The DNA must be purified after exiting the aerosol collector to prevent inhibition of the enzymatic reaction. Traditional solid-phase extraction results in a large loss of sample. In this flow-through system, we perform sample purification, concentration and amplification in one reactor, which minimizes the loss of material. The sample from the aerosol collector is mixed with a denaturation solution prior to flowing through a capillary packed with silica beads. The DNA adheres to the silica beads allowing the environmental contaminants to be flushed to waste while effectively concentrating the DNA on the silica matrix. The adhered DNA is amplified while on the surface of the silica beads, resulting in a lower limit of detection than an equivalent eluted sample. Thus, this system is beneficial since more DNA is available for amplification, less reagents are utilized, and contamination risks are reduced.

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Section: Post-pcr Detectionmentioning

confidence: 99%

Flow through PCR module of BioBriefcase

et al. 2005

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“…28,29 In 1995, Dovichi et al reported the first separation of DNA sequencing fragments at 60˚C with non-cross-linked polymer capillary electrophoresis. 30 This temperature was sufficiently high to eliminate any sequencing artifacts, thereby generating a high-quality sequence. Analytical chemists seized the opportunity, addressing the problem of achieving a high throughput with good sensitivity, says H. Kambara at Hitachi's Central Research Laboratory, Japan.…”

Section: ·3 Capillary Electrophoresis For Dna Sequencingmentioning

confidence: 99%

The Human Genome Project: the Role of Analytical Chemists

Thangadurai¹

2004

ANAL. SCI.

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“…Zhang et al 1995Zhang et al , 1999Bashkin et al 1996a,b;Carrilho et al 1996;Kheterpal et al 1996;Dovichi 1997Dovichi , 1999Kheterpal and Mathies 1999;Pang et al 1999;Crabtree et al 2000;Gao and Yeung 2000;McWhorter and Soper 2000) to provide high throughput analysis of DNA fragments.…”

Section: Capillary-based Sequencersmentioning

confidence: 99%

“…At the detection end of the capillaries, the samples flow through a sheath flow cuvette detector (originally developed by Dr. Norman Dovichi, University of Alberta, Edmonton, Canada; http://www. chem.ualberta.ca/faculty/dovichi.htm; Swerdlow et al 1991;Zhang et al 1995;Service 1998c) to eliminate capillary wall scattering and to provide continuous excitation and detection. Uncoated capillaries are used and are dynamically coated with a polymer such as POP-6 to control electro-osmotic flow.…”

Section: Capillary-based Sequencersmentioning

confidence: 99%

Automation for Genomics, Part Two: Sequencers, Microarrays, and Future Trends

Meldrum¹

2000

Genome Res.

116

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Automation for genomics has enabled a 43-fold increase in the total finished human genomic sequence in the world in the past four years. This is the second half of a two-part, noncomprehensive review that presents an overview of different types of automation equipment used in genome sequencing. The first part of the review, published in the previous issue, focused on automated procedures used to prepare DNA for sequencing or analysis. This second part of the review presents a look at available DNA sequencers and array technology and concludes with a look at future technologies. Alternate sequencing technologies including mass spectrometry, biochips, and single molecule analysis are included in this review.Sequencing data, essential for many of the medical breakthroughs of today and tomorrow, is currently accumulating at an exponential rate, largely because of advances in sequencing methods and laboratory automation. Instruments are available that can automate nearly every step in the large-scale sequencing process.The first article in this two-part review (Meldrum 2000) presented a description of automation designed for isolating DNA, cloning or amplifying DNA, preparing enzymatic sequencing reactions, and purifying DNA. Part one of the review also showed that the majority of genome centers use both in-house automation and commercial automation (Collins et al. 1998;Wendl et al. 1998;Mullikin and McMurragy 1999;Spurr et al. 1999; see http://www.genome.org for a supplementary table providing a snapshot view of automation used at a number of different genome centers).In this, the second part of the review, the focus is on automation after DNA preparation and sequencing reactions-on obtaining the sequence and its analysis. A discussion of future technologies for DNA sequencing projects is also included. (See the web sites referenced for photos or further details on the instruments presented.)

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Use of Non-Cross-Linked Polyacrylamide for Four-Color DNA Sequencing by Capillary Electrophoresis Separation of Fragments up to 640 Bases in Length in Two Hours

Cited by 124 publications

References 0 publications

Flow through PCR module of BioBriefcase

Flow through PCR module of BioBriefcase

The Human Genome Project: the Role of Analytical Chemists

Automation for Genomics, Part Two: Sequencers, Microarrays, and Future Trends

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