Use of Native Lactococci as Vehicles for Delivery of DNA into Mammalian Epithelial Cells

Guimarães, Valeria; Innocentin, Silvia; Lefèvre, François; Azevedo, Vasco; Wal, Jean‐Michel; Langella, Philippe; Châtel, Jean-Marc

doi:10.1128/aem.01325-06

Cited by 57 publications

(59 citation statements)

References 44 publications

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“…9 In similar experiments, the use of L. lactis LL(pLIG:BLG) to deliver a functional eukaryotic blg gene led to both expression and secretion of BLG. 3 In our experiments, the BLG cDNA was detected in DNA extracted from the small intestinal wall that was washed of gut contents 72 h after the last gavage. This result can be considered as an indirect evidence of plasmid transfer from lactococci to epithelial mouse intestinal cells.…”

Section: Discussionmentioning

confidence: 99%

“…We have recently shown in vitro that transformation of Caco-2 cells is more efficient when the plasmid is inside the bacterium than just associated with bacteria. 3 This could partly explain the lack of GFP expression after intragastric gavage of naked DNA.…”

Section: Discussionmentioning

confidence: 99%

“…3 Briefly, pLIG:BLG1 is a cointegrate obtained by fusing pIL253, 34 an erythromycin-resistant plasmid replicating in L. lactis, with pcDNA3BLG5, 35 an ampicillin-resistant plasmid replicating in E. coli and carrying an eukaryotic BLG expression cassette. pLIG1 is a cointegrate obtained by fusing pIL253 and pcDNA3.…”

Section: Plasmids Bacterial Strains Media and Growth Conditionsmentioning

confidence: 99%

“…Recently, we explored the potential use of noninvasive L. lactis strains as a DNA delivery vehicle. 3 We constructed one Escherichia coli L. lactis shuttle plasmid, pLIG:BLG1, which contains a eukaryotic expression cassette with the cDNA of bovine b-lactoglobulin (BLG). We first showed that the resulting L. lactis strain MG1363(pLIG:BLG1) was not able to express BLG.…”

Section: Introductionmentioning

confidence: 99%

“…[8][9][10] Nevertheless, it has been demonstrated in vitro that noninvasive bacteria as L. lactis or E. coli are able to transfer genetic material. 3,9,11 In vivo, E. coli has been used as vector for DNA vaccines by intramuscular, intraperitoneal or subcutaneous administrations. 12,13 Here, we explored the ability of not genetically modified L. lactis to deliver plasmid in vivo to mice.…”

Section: Introductionmentioning

confidence: 99%

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In vivo transfer of plasmid from food-grade transiting lactococci to murine epithelial cells

Pothelune

Ah‐Leung

et al. 2008

Gene Ther

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We recently demonstrated that noninvasive food-grade Lactococcus lactis (L. lactis) can deliver eukaryotic expression plasmid in mammalian cells in vitro. Here, we evaluated, in vivo, whether a eukaryotic expression plasmid carried by lactococci can translocate to the epithelial cells of the intestinal membrane. The strain LL(pLIG:BLG1) carrying one plasmid containing a eukaryotic expression cassette encoding b-lactoglobulin (BLG), a major allergen of cow's milk, was orally administered by gavage to mice. BLG cDNA was detected in the epithelial membrane of the small intestine of 40% of the mice and BLG was produced in 53% of the mice. Oral administration of LL(pLIG:BLG1) induced a low and transitory Th1-type immune response counteracting a Th2 response in case of further sensitization. We demonstrated for the first time the transfer of a functional plasmid to the epithelial membrane of the small intestine in mice by noninvasive food-grade lactococci.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Plasmids Bacterial Strains Media and Growth Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In vivo transfer of plasmid from food-grade transiting lactococci to murine epithelial cells

Pothelune

Ah‐Leung

et al. 2008

Gene Ther

View full text Add to dashboard Cite

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Engineering Lactic Acid Bacteria and Bifidobacteria for Mucosal Delivery of Health Molecules

Allain¹,

Aubry²,

Natividad³

et al. 2015

Biotechnology of Lactic Acid Bacteria

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Insight of oral vaccines as an alternative approach to health and disease management: An innovative intuition and challenges

Debnath

Thakur

Khushboo

et al. 2021

Biotech & Bioengineering

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Vaccination is the most suitable and persuasive healthcare program for the prohibition of various deadly diseases. However, the higher production cost and purification strategies are out of reach for the developing nations. In this scenario, development of edible vaccine turns out to be the most promising alternative for remodeling the pharmaceutical industry with reduced production and purification costs. Generally, oral route of vaccination is mostly preferred due to its safety, compliance, low manufacturing cost and most importantly the ability to induce immunity in both systemic and mucosal sites. Genetically modified microorganisms and plants could efficiently be used as vehicles for edible vaccines. Edible vaccines are supposed to reduce the risk associated with traditional vaccines. Currently, oral vaccines are available in the market for several viral and bacterial diseases like cholera, hepatitis B, malaria, rabies etc. Herein, the review focuses on the breakthrough events in the area of edible vaccines associated with dietary microbes and plants for better control over diseases.

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Use of Native Lactococci as Vehicles for Delivery of DNA into Mammalian Epithelial Cells

Cited by 57 publications

References 44 publications

In vivo transfer of plasmid from food-grade transiting lactococci to murine epithelial cells

In vivo transfer of plasmid from food-grade transiting lactococci to murine epithelial cells

Engineering Lactic Acid Bacteria and Bifidobacteria for Mucosal Delivery of Health Molecules

Insight of oral vaccines as an alternative approach to health and disease management: An innovative intuition and challenges

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