Use of Nanoparticles Based on Metals in the Composition of Modified Media for Microclonal Reproduction of Woody Plants: Preliminary Results

Abstract

Study of the problems and development of methods for obtaining tree-like plant material using a culture of isolated tissues that promotes rapid mass reproduction and healing of pure valuable tree genotypes for the restoration of forest plantations, as well as in selection is highly relative issue. We have prepared MS-modified nutrient media modified with metal nanoparticles to increase the efficiency of microclonal propagation of woody cultures. It is assumed that this modification will enhance the growth and morphogenesis of plants, as well as reduce the microbial load, both in the nutrient medium and in the resulting explants. In addition, reducing the cost of explants produced by replacing nanoparticles with traditionally used growth stimulants with colloidal solutions and reducing the number of disinfection procedures can significantly increase the attractiveness of the microclonal propagation method for forestry and agriculture. Preliminary studies have showed an increase in the survival of white poplar and aspen hybrid microclones, which indicates the promise of the chosen approach..