Use of multigeneration-family molecular dog leukocyte antigen typing to select a hematopoietic cell transplant donor for a dog with T-cell lymphoma

Lupu, Marilena; Sullivan, Edmund W.; Westfall, Theresa; Little, Marie Térèse; Weigler, Benjamin J.; Moore, Peter F.; Stroup, P.; Zellmer, Eustacia; Kuhr, Christian S.; Storb, Rainer

doi:10.2460/javma.228.5.728

Cited by 26 publications

(31 citation statements)

References 26 publications

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“…The use of apheresis devices for plasma exchanges proved feasible for the treatment of dogs with multiple myeloma 67 and malignant lymphoma 68 . Our group has already reported the use of aphereses for autologous 48 and allogeneic 56 PBMC collections for the treatment of dogs with malignant lymphoma in chemotherapy‐induced remission. Twenty‐five percent of the recipients of the autologous PBMC grafts 48 and the recipient of the allogeneic G‐PBMC graft 56 became disease‐free, long‐term survivors.…”

Section: Discussionmentioning

confidence: 99%

“…Our group has already reported the use of aphereses for autologous 48 and allogeneic 56 PBMC collections for the treatment of dogs with malignant lymphoma in chemotherapy‐induced remission. Twenty‐five percent of the recipients of the autologous PBMC grafts 48 and the recipient of the allogeneic G‐PBMC graft 56 became disease‐free, long‐term survivors. These data provide evidence that PBMC apheresis technology also can be translated to the field of veterinary oncology for the treatment of dogs with hematologic malignancies.…”

Section: Discussionmentioning

confidence: 99%

“…All dogs had received antihelmintic treatments and complete immunizations against parvovirus, distemper, hepatitis, parainfluenza, leptospirosis, rabies, and papilloma virus, and were monitored for disease at least 60 days before apheresis. A private dog that served as allogeneic HCT donor 56 was screened for Dirofilaria immitis, Ehrlichia canis, E. equi, Babesia canis, Borrelia burgdorferi , and Rickettsia rickettsi in order to avoid transmission of any potential blood‐borne pathogens to the recipient. Food was withheld from all dogs 12 hours before apheresis and water was provided ad libitum.…”

Section: Methodsmentioning

confidence: 99%

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Principles of Peripheral Blood Mononuclear Cell Apheresis in a Preclinical Canine Model of Hematopoietic Cell Transplantation

Lupu

Gooley

Zellmer

et al. 2008

Veterinary Internal Medicne

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Background: Preclinical studies of peripheral blood mononuclear cell (PBMC) transplantation conducted in a well-established canine hematopoietic cell transplantation (HCT) model have been successfully translated to human patients over the past 5 decades.Objective: We retrospectively investigated the safety and feasibility of PBMC apheresis in the canine model of HCT by analyzing apheresis parameters, cell yields, and the impacts of donor-related and apheresis-related variables on collection yields and donor stability.Animals: One hundred and twenty dogs that underwent PBMC aphereses were evaluated. Methods: Aphereses were performed with a COBE Spectra blood separator and a central dual-lumen catheter, with or without recombinant canine granulocyte colony-stimulating factor (rcG-CSF) stem cell mobilization.Results: Aphereses from dogs not given rcG-CSF yielded an average volume of 280 AE 42 mL containing an average of 15,086 AE 9,834 leukocytes/mL. Aphereses from dogs given rcG-CSF yielded an average volume of 261 AE 55 mL containing an average of 39,711 AE 24,488 leukocytes/mL. Higher pre-apheresis white blood cell (WBC) counts correlated with higher apheresis WBC yields (R 5 0.50, Po.0001). The correlations of collection time, inlet volume, and collection flow rate on WBC yields were statistically significant but only weak to moderate in magnitude (R 5 0.34, P 5 .0001; R 5 0.38, P 5 .0006; R 5 0.26, P 5 .002, respectively) as were the correlations of collection time and inlet volume on collection volumes (R 5 0.30, P 5 .002; R 5 0.42, Po.0001, respectively). All dogs recovered promptly after PBMC aphereses and catheter removal, without complications.Conclusions and Clinical Importance: These data may be useful for translating PBMC apheresis technology to the field of veterinary oncology for the treatment of dogs with hematologic malignancies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Principles of Peripheral Blood Mononuclear Cell Apheresis in a Preclinical Canine Model of Hematopoietic Cell Transplantation

Lupu

Gooley

Zellmer

et al. 2008

Veterinary Internal Medicne

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“…5 Given this research data and recent reports documenting the successful harvesting of peripheral blood CD34þ progenitor cells or whole bone marrow and reinfusion after myeloablative therapy in a clinical setting, 3,4,6 North Carolina State University began treating dogs diagnosed with B-and T-cell lymphoma with autologous peripheral blood progenitor cell transplant following lethal total body irradiation (TBI). In this report, we document the bone marrow toxicities associated with the pretransplant TBI and the subsequent hematopoietic reconstitution in the ensuing weeks.…”

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confidence: 99%

Hematologic Changes After Total Body Irradiation and Autologous Transplantation of Hematopoietic Peripheral Blood Progenitor Cells in Dogs With Lymphoma

et al. 2011

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Dogs with and without lymphoma have undergone hematopoietic cell transplantation in a research setting for decades. North Carolina State University is currently treating dogs with B-and T-cell lymphoma in a clinical setting with autologous peripheral blood progenitor cell transplants, using peripheral blood CD34þ progenitor cells harvested using an apheresis machine. Complete blood counts were performed daily for 15 to 19 days posttransplantation to monitor peripheral blood cell nadirs and subsequent CD34þ cell engraftment. This study documents the hematologic toxicities of total body irradiation in 10 dogs and the subsequent recovery of the affected cell lines after peripheral blood progenitor cell transplant, indicating successful CD34þ engraftment. All peripheral blood cell lines, excluding red blood cells, experienced grade 4 toxicities. All dogs had ! 500 neutrophils/ml by day 12, while thrombocytopenia persisted for many weeks. All dogs were clinically normal at discharge.

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“…Recently, both auto-and allo-HSCT has begun to be clinically employed for the treatment of non-Hodgkin lymphoma in companion dogs. 20,21 These dogs offer an opportunity to evaluate the impact of FMC in the management of GVHD and in modifying conditioning regimens to decrease toxicity of the therapy while optimizing rates of engraftment and minimizing GVHD.…”

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confidence: 99%

The health effects of fetal microchimerism can be modeled in companion dogs

et al. 2013

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Fetal microchimerism (FMC) has been described to have a range of effects on health and disease. Y-chromosomal DNA has been detected in Golden Retrievers suggesting persistent FMC. In that report, nine dogs had evidence of microchimerism without prior pregnancy. To further understand this finding, a dam with prior male live births giving birth to her fourth litter of puppies, all females, was evaluated for FMC along with two of her daughters. All three female dogs had evidence of Y-chromosomal DNA in their blood. This suggests that male cells carried by the dam from previous pregnancy trafficked to her daughters to establish microchimerism in younger siblings. Companion dogs share many of the same cancers as humans, have out-bred genetics, and share the human environment, making them optimal models of human disease. Understanding the impact of FMC on health and disease of dogs could elucidate mechanisms useful for clinical interventions in humans.

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Use of multigeneration-family molecular dog leukocyte antigen typing to select a hematopoietic cell transplant donor for a dog with T-cell lymphoma

Cited by 26 publications

References 26 publications

Principles of Peripheral Blood Mononuclear Cell Apheresis in a Preclinical Canine Model of Hematopoietic Cell Transplantation

Principles of Peripheral Blood Mononuclear Cell Apheresis in a Preclinical Canine Model of Hematopoietic Cell Transplantation

Hematologic Changes After Total Body Irradiation and Autologous Transplantation of Hematopoietic Peripheral Blood Progenitor Cells in Dogs With Lymphoma

The health effects of fetal microchimerism can be modeled in companion dogs

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